Ecogenotoxicity of environmentally relevant atrazine concentrations: A threat to aquatic bioindicators

Souza, Victor Ventura de; Souza, Tatiana da Silva; Campos, José Marcello Salabert de; Oliveira, Luiza Araújo de; Ribeiro, Yves Moreira; Hoyos, Daniela Chemin de Melo; Xavier, Rogéria Maura Panzini; Charlie-Silva, Ives; Lacerda, Samyra Maria dos Santos Nassif

doi:10.1016/j.pestbp.2022.105297

Cited by 10 publications

(2 citation statements)

References 98 publications

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“…The appropriately disaggregated cells were rapidly, efficiently and quantitatively analysed by multicolour FC (Auguste et al 2020 ; Barmo et al 2013 ; Canesi et al 2008 ; Ciacci et al 2011 ; Souza et al 2023 ). This methodology that can be successfully applied to analyse hepatopancreatic cells from terrestrial isopods in order to conduct a so-called environmental diagnosis from a cytologic/histologic point of view, aimed to protect environmental welfare and human health (Abelouah et al 2023 ; da Silva Souza et al 2020 ; Plutino et al 2022 ; Vieira et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings

Panza,

Montanari,

Lopez

et al. 2024

Environ Sci Pollut Res

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Several studies have reported the high bioindication capacity of Isopoda (Crustacea, Oniscidea), which is related to their important ability to accumulate contaminants, usefulness in soil ecotoxicology and bioindication activities. Any change in the isopod population, diversity and life cycle can indicate relevant pollution levels. The analysis of target tissues, such as the hepatopancreas, is another emerging approach (from a cytologic/histological level) to detect contaminant accumulation from different sources. In this study, tissue disaggregation procedures were optimised in the hepatopancreas, and flow cytometry (FC) was applied to detect cell viability and several cell functions. After disaggregation, two hepatopancreatic cell types, small (S) and big (B), were still recognisable: they differed in morphology and behaviour. The analyses were conducted for the first time on isopods from sites under different conditions of ecological disturbance through cytometric re-interpretation of ecological-environmental parameters. Significant differences in cell functional parameters were found, highlighting that isopod hepatopancreatic cells can be efficiently analysed by FC and represent standardisable, early biological indicators, tracing environmental-induced stress through cytologic/histologic analyses.

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Section: Discussionmentioning

confidence: 99%

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings

Panza,

Montanari,

Lopez

et al. 2024

Environ Sci Pollut Res

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show abstract

“…The appropriately disaggregated cells were rapidly, e ciently and quantitatively analysed by multicolour FC (Auguste et al, 2020;Barmo et al, 2013;Canesi et al, 2008;Ciacci et al, 2011;Souza et al, 2023).…”

Section: Statistical Analysesmentioning

confidence: 99%

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial Isopods as efficient environmental bioindicators in ex vivo settings

Panza

Montanari

Lopez

et al. 2023

Preprint

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Several studies report the high capacity of bioindication of Isopoda (Crustacea, Oniscidea), related to their important ability to accumulate contaminants, useful in soil ecotoxicology and in bioindication activities. Any change in the Isopods population, diversity, and life cycle can indicate relevant pollution levels. The analysis of target tissues, as hepatopancreas, is another emerging approach (from a cytologic/histologic level) to detect contaminant accumulation from different sources. In this study, tissue disaggregation procedures were optimised on hepatopancreas, and Flow Cytometry (FC) was applied to detect cell viability and several cell functions. After disaggregation, two hepatopancreatic cell types, Small (S) and Big (B), were still recognizable: they differ in morphology and behaviour. The analyses were conducted for the first time on Isopods from sites at different conditions of ecological disturbance through a cytometric re-interpretation of ecological-environmental parameters. Significant differences in cell functional parameters were found, highlighting that Isopod hepatopancreatic cells can be efficiently analysed by FC and represent standardisable, early biologic indicators, tracing environmental-induced stress through cytologic/histologic analyses.

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The use of integrative tools and multiple models for aquatic environmental quality assessment: a case study of the Mirim Lagoon, Southern Brazil

da Silva,

Martins,

Guedes

et al. 2024

Environ Monit Assess

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Ecogenotoxicity of environmentally relevant atrazine concentrations: A threat to aquatic bioindicators

Cited by 10 publications

References 98 publications

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings

Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial Isopods as efficient environmental bioindicators in ex vivo settings

The use of integrative tools and multiple models for aquatic environmental quality assessment: a case study of the Mirim Lagoon, Southern Brazil

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