Ecofriendly laccases treatment to challenge micropollutants issue in municipal wastewaters

Spina, Federica; Gea, Marta; Bicchi, Carlo; Cordero, Chiara Emilia Irma; Schilirò, Tiziana; Varese, Giovanna Cristina

doi:10.1016/j.envpol.2019.113579

Cited by 39 publications

(22 citation statements)

References 141 publications

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“…Major primary endpoints assessment from samples at WWTPs and rivers were estrogenicity and androgenicity and analyses of such samples containing different chemical mixtures 29,[39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57] (Fig. 2).…”

Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

“…only determining endpoint measures such as estrogenicity or androgenicity for suspected endocrine disrupting chemicals (EDCs). 30,40 Predominantly, studies looking into possible MOAs of compounds within the CEC list such as diclofenac, carbamazepine, venlafaxine and trimethoprim primarily investigated estrogenicity and androgenicity of the sample mixtures wherein they are present. While some studies have not clearly stated reason for the choice of bioassay, this may also be inuenced by the regulations in the WFD, listing possible bioassays that should be tested rather than careful analysis of the chemistry of the analytes.…”

Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

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Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

et al. 2021

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Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

et al. 2021

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“…On the contrary, brown-rot fungi mostly colonize conifers and primarily degrade hemicellulose and cellulose, leaving a brown coloured residual wood enriched in the darker lignin [ 47 ]. However, both white-rot fungi and some brown-rot fungal species, such as Gloeophyllum trabeum (Bourdot & Galzin) Domański, Orloś & Skirg [ 48 ], are particularly predisposed to lignin degradation/mineralization due to their production of several oxidoreductase enzymes for conversion of lignin to H 2 O and CO 2 [ 48 – 50 ]. Examples of common wood decaying white-rot fungi are Phanerochaete chrysosporium Burds., Armillaria sp.…”

Section: Lignin Modifying Fungi and Ligninolytic Consortiummentioning

confidence: 99%

“…Figure adapted from Alcalde [87] degrade hemicellulose and cellulose, leaving a brown coloured residual wood enriched in the darker lignin [47]. However, both white-rot fungi and some brown-rot fungal species, such as Gloeophyllum trabeum (Bourdot & Galzin) Domański, Orloś & Skirg [48], are particularly predisposed to lignin degradation/mineralization due to their production of several oxidoreductase enzymes for conversion of lignin to H 2 O and CO 2 [48][49][50] Production of LEs has not yet been reported in Chytridiomycota [51], whereas reports are increasing from the Ascomycota (mould group) and Mucoromycota [52]. The soft rot fungi (ascomycetes) such as Xylaria, Fusarium, Penicillium, Trichoderma, and Aspergillus species can degrade the softer section of wood that is constituted by a lower proportion of lignin [53].…”

Section: Lignin Modifying Fungimentioning

confidence: 99%

Genome-based engineering of ligninolytic enzymes in fungi

et al. 2021

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Background Many fungi grow as saprobic organisms and obtain nutrients from a wide range of dead organic materials. Among saprobes, fungal species that grow on wood or in polluted environments have evolved prolific mechanisms for the production of degrading compounds, such as ligninolytic enzymes. These enzymes include arrays of intense redox-potential oxidoreductase, such as laccase, catalase, and peroxidases. The ability to produce ligninolytic enzymes makes a variety of fungal species suitable for application in many industries, including the production of biofuels and antibiotics, bioremediation, and biomedical application as biosensors. However, fungal ligninolytic enzymes are produced naturally in small quantities that may not meet the industrial or market demands. Over the last decade, combined synthetic biology and computational designs have yielded significant results in enhancing the synthesis of natural compounds in fungi. Main body of the abstract In this review, we gave insights into different protein engineering methods, including rational, semi-rational, and directed evolution approaches that have been employed to enhance the production of some important ligninolytic enzymes in fungi. We described the role of metabolic pathway engineering to optimize the synthesis of chemical compounds of interest in various fields. We highlighted synthetic biology novel techniques for biosynthetic gene cluster (BGC) activation in fungo and heterologous reconstruction of BGC in microbial cells. We also discussed in detail some recombinant ligninolytic enzymes that have been successfully enhanced and expressed in different heterologous hosts. Finally, we described recent advance in CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR associated) protein systems as the most promising biotechnology for large-scale production of ligninolytic enzymes. Short conclusion Aggregation, expression, and regulation of ligninolytic enzymes in fungi require very complex procedures with many interfering factors. Synthetic and computational biology strategies, as explained in this review, are powerful tools that can be combined to solve these puzzles. These integrated strategies can lead to the production of enzymes with special abilities, such as wide substrate specifications, thermo-stability, tolerance to long time storage, and stability in different substrate conditions, such as pH and nutrients.

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“…pH is a crucial factor governing the conversion kinetics of phenolic pollutants with HAs in fungal laccasetriggered humi cation systems (Gan et al, 2019;Spina et al, 2020;Wang et al, 2018). In this study, the effect of varying pH levels on the conversion kinetics of E2 was quantitatively analyzed in Tvlaccatalyzed humi cation processes by HPLC.…”

Section: Tvlac-evoked Humi Cation Kinetics Of E2 With Has At Varying mentioning

confidence: 99%

Effect of Two Humic Acids on Laccase-Driven 17β-Estradiol Conversion Kinetics and Polymerization Products at Varying pH Levels

Kim

Zhu

et al. 2021

Preprint

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Estrogens with tremendous ecological risks are proverbially found in water. Laccase can drive humification of estrogens to reduce their ecotoxicity and removability, but little investigation existed in exploring the effect of humic acids (HAs) on E2 conversion kinetics and polymerization products at different pH conditions. Here, Trametes versicolor laccase (Tvlac) was capable of efficiently converting a representative estrogen, 17β-estradiol (E2) with two different HAs, and the process followed a pseudo-first-order kinetics. The velocity constants were respectively 0.048, 0.022, and 0.020 min− 1 for HA-free, peat-derived HA, and commercial HA at pH 5.0. The changing pH not only impacted E2 coupling kinetics in Tvlac-evoked humification, but altered the aromaticity and humification degrees of HAs. A total of five intermediate species including estrone (E1), E2 dimer, trimer, and tetramer, as well as E1-E2 cross-linked products were tentatively detected, in which the dominating species were E2 self-oligomers resulting from radical-centered carbon-carbon/oxygen stepwise polymerization routes. Yields of dimeric, trimeric, and tetrameric species with increased molecular sizes were the highest at pH 5.0 in the given pH values, and they were readily handled by precipitation and filtration. Especially E2 was able to be covalently incorporated into humic constituents to generate new humified co-polymers, thereby accelerating E2 humification and detoxification. These findings demonstrate that pH exhibits a far-reaching influence on the conversion kinetics, humification degrees, and products distribution of E2 and HAs in Tvlac-reinforced polyreaction. Thus, there is need to reappraise the fate and transport of estrogens with HAs present in natural water at varying pH levels.

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Ecofriendly laccases treatment to challenge micropollutants issue in municipal wastewaters

Cited by 39 publications

References 141 publications

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

Genome-based engineering of ligninolytic enzymes in fungi

Effect of Two Humic Acids on Laccase-Driven 17β-Estradiol Conversion Kinetics and Polymerization Products at Varying pH Levels

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