Ecofriendly green conversion of potato peel wastes to high productivity bacterial cellulose

Abdelraof, Mohamed; Hasanin, Mohamed S.; El‐Saied, Houssni

doi:10.1016/j.carbpol.2019.01.095

Cited by 146 publications

(75 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many sources such as plants, animal cells, and microorganisms have been investigated for their ability to produce valuable metabolites [10][11][12]. Recently, microorganisms have proved to be excellent source for production of several commercial enzymes with a wide variety of applications [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Hamed

Khedr

Abdelraof

2020

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

Background Plasmid propagation in recombination strains such as Escherichia coli DH5α is regarded as a beneficial instrument for stable amplification of the DNA materials. Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, transposable element (Tn5)) and M13 sequence via α-complementation mechanism in order to activate β-d-galactosidase gene in DH5α strain (non-lactose-fermenting host). Results Trans-conjugation with pGEM-T allows correction of LacZ gene deletion through Tn5, and successful trans-conjugants in DH5α host cells can be able to produce active enzyme, thus described as lactose fermenting strain. The intracellular β-galactosidase was subjected to precipitation by ammonium sulfate and subsequently gel filtration, and the purified enzyme showed a molecular weight of approximately 72-kDa sodium dodecyl sulfate-polyacrylamid gel electrophoresis. The purified enzyme activity showed an optimal pH and temperature of 7.5 and 40 °C, respectively; it had a high stability within pH 6–8.5 and moderate thermal stability up to 50 °C. Conclusion Trans-conjugant of E. coli DH5α- lacZ∆M15 was successfully implemented. UV mutagenesis of the potent trans-conjugant isolate provides an improvement of the enzyme productivity. The enzymatic competitive inhibition by d-galactose and hydrolysis of lactose at ambient temperatures could make this enzyme a promising candidate for use in the dairy industry.

show abstract

Section: Introductionmentioning

confidence: 99%

Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Hamed

Khedr

Abdelraof

2020

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Best experimental variables levels for maximizing BC production were predicted through analysis of these plots in combination with numerical optimization for each variable and desirability analysis. Interestingly, BC production by SCB immobilized cells after statistical optimization is approximately 5 times higher than of those observed in the HS medium (1.21 g/L).As reported before, generation of gluconic acid from the catabolism of glucose during the BC biosynthesis is regarded as the most common drawback due to the rapid decrease in the pH value of the medium which results in feedback inhibition of the BC synthesis (Bilgi et al, 2016, Abdelraof et al, 2019a, Abdelraof et al, 2019b.…”

Section: Optimization Of the Scb-immobilized Cell System Using Plackementioning

confidence: 84%

Immobilization of Glucanobacter Xylinum Onto Natural Polymers to Enhance the Bacterial Cellulose Productivity

Abdelraof

El‐Saied²,

Hasanin

2020

Preprint

Self Cite

View full text Add to dashboard Cite

Bacterial cellulose (BC) has profound applications in different sectors of biotechnology due to its unique properties preferring it about plant cellulose. Although this polymer is extremely important in various applications, many problems still hinder the sustainable production in terms of increasing productivity and low-cost production. In order to overcome these problems, this study will focuses on the continuous production of cellulose using immobilized Glucanobacter xylinum cells onto Sugar Cane Bagasse (SCB) and Ca-alginate beads. Comparatively, adsorption of Glucanobacter xylinum cells to the cavum of stalk cells of SCB could be efficiently stable while, entrapment of cells onto Ca-alginate has drawback observed by the rapid disruption and instability of the beads in the Potato Peel Waste (PPW) culture medium. Our findings demonstrate that a combination between alternative low-cost medium with continuous production mode by immobilization onto inexpensive natural polymer can promote a sustainable bioprocess and reduction the production cost.

show abstract

“…Black liquor is consisted of dissolved lignin, silica, and sodium sulphite as well as some soluble elements (Na, K, P, Cl, and S) with different ratios according to pulping method and raw material used (Dayton and Frederick 1996;Maček 1999). It is a highly hazardous and corrosive by-product with a high silica content (Tutuş and Eroğlu 2003;Hasanin et al 2018b;Abdelraof et al 2019a;Abdelraof et al 2019b;Hasanin et al 2020). The extraction of silica from black liquor is an important pretreatment to decrease the black liquor corrosion effect.…”

Section: Introductionmentioning

confidence: 99%

Sustainable hybrid silica extracted from rice husk with polyvinyl alcohol and nicotinic acid as multi adsorbent for textile wastewater treatment

Hasanin

2020

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

A new eco-friendly compound was prepared for the treatment of textile wastewater containing mixed dyes with various ranges of toxicity. Porous silica was extracted from a black liquor by-product using a simple method and characterized by porous morphology (the pore size ranged between 12 and 41 nm). The silica is the main corrosive agent present in the black liquor; thus, the extraction of silica from the black liquor was considered detoxification process. The extracted porous silica was used as a precursor material to prepare the hybrid material based on polyvinyl alcohol (PVA) as a binder polymer and functionalized by nicotinic acid. The multifunction prepared hybrid was characterized by FT-IR, TGA, DTGA, SEM, and EDX. The porous size of the prepared hybrid varied from 96 nm to 620 nm and presents a high thermal stability in comparison with its parent materials. The adsorption of cationic and anionic dyes was carried out. The adsorption kinetics parameters were fitted with pseudo-firstorder and pseudo-second-order kinetic models for methyl orange (MO) and methylene blue (MB), respectively. The adsorption parameters indicated that the Langmuir model is better to describe the adsorption of dyes on the hybrid material. The maximum adsorption capacity was 484 and 771 mg/g for MO and MB, respectively.

show abstract

Ecofriendly green conversion of potato peel wastes to high productivity bacterial cellulose

Cited by 146 publications

References 57 publications

Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Immobilization of Glucanobacter Xylinum Onto Natural Polymers to Enhance the Bacterial Cellulose Productivity

Sustainable hybrid silica extracted from rice husk with polyvinyl alcohol and nicotinic acid as multi adsorbent for textile wastewater treatment

Contact Info

Product

Resources

About