Eco-geographical diversity of cowpea bradyrhizobia in Senegal is marked by dominance of two genetic types

Wade, Tatiana Krasova; Quéré, Antoine Le; Laguerre, Gisèle; N’Zoué, Angèle; Ndione, Jacques André; doRego, Francis; Sadio, Oumar; Ndoye, Ibrahima; Neyra, Marc

doi:10.1016/j.syapm.2013.10.002

Cited by 29 publications

(41 citation statements)

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“…This symbiotic performance was also depending on variety and location. Although based on the cropping systems, the dominance of IGS type I at Cinzana Agronomic Research Station was consistent with results obtained by Krasova- Wade et al (2003), Kanté (2004) and Kouyaté (2006) and Krasova Wade et al (2014). It was a very competitive strain in the Sudano-Sahelian zone of Mali, Niger and Senegal under 600-700 mm of rain falls.…”

Section: Diversity Of Native Bradyrhizobia Populationssupporting

confidence: 88%

International Research Journal of Agriculture and Soil Science

2014

Int. Res. J. Agric. Sci. Soil Sci.

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Cowpea is an important food grain legume in the Sudano Sahelian zone of Mali. So far, an attention has not been addressed to the diversity of bradyrhizobia nodulating cowpea in this area. In order to fill this gap, effects of 3 cowpea varieties (IT89KD-374, CZ1-94-23-1 and CZ11-94-5C) on the diversity of native bradyrhizobia has been studied in 3 cropping systems from 2007 to 2008 at Cinzana Agronomic Research Station. The objective of the study was to identify effective bradyrhizobia strains nodulating cowpea and their impact on the intercropped or subsequent millet yield in the main cropping systems. Experimental design was a Randomized Complete Block Design using a factorial combination of 3 cowpea varieties and 3 cropping systems in 4 replications. PCR-RFLP and sequencing of the 16S -23S of rDNA IGS were used to identify bradyrhizobia strains associated with cowpea varieties. Six IGS types have been identified. IGS type I (strain Bradyrhizobium sp.ORS 3642) was the most competitive regardless of cropping system and cowpea variety. The greater diversity averaged bradyrhizobia was found in variety IT89KD-374 (H' means = 0.39). Best millet grain yield in intercropped system (1520 kg ha -1 ) was obtained with cowpea variety IT89KD-374. The best above ground dry biomass production of subsequent millet (6 210 kg ha -1 ) has been obtained under CZ11-94-5C -millet rotation. Types IGS IV and VI (ORS 3637 and STM 3378 strains respectively) appeared to be responsible for the performance of the Cowpea-millet rotation and cowpea variety CZ11-94-5C.

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Section: Diversity Of Native Bradyrhizobia Populationssupporting

confidence: 88%

International Research Journal of Agriculture and Soil Science

2014

Int. Res. J. Agric. Sci. Soil Sci.

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“…symbionts in three eco-regions of China [ 74 ]. The geographical pattern of symbionts observed in our study could be linked to the annual rainfall characteristics of each site, as observed for Vigna unguiculata symbionts in Senegal [ 75 ]. Indeed, the M. plurifarium strains were only found in the center of the Sudano-Sahelian zone where annual rainfall is between 500 and 900 mm, while no strain of this clade was found in the Kamb and Dahra sites, located in the arid regions of the sahelian zone where annual rainfall varied between 250 and 500 mm ( Fig.…”

Section: Discussionsupporting

confidence: 53%

Genetic and Genomic Diversity Studies of Acacia Symbionts in Senegal Reveal New Species of Mesorhizobium with a Putative Geographical Pattern

Diouf

Klonowska

et al. 2015

PLoS ONE

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Acacia senegal (L) Willd. and Acacia seyal Del. are highly nitrogen-fixing and moderately salt tolerant species. In this study we focused on the genetic and genomic diversity of Acacia mesorhizobia symbionts from diverse origins in Senegal and investigated possible correlations between the genetic diversity of the strains, their soil of origin, and their tolerance to salinity. We first performed a multi-locus sequence analysis on five markers gene fragments on a collection of 47 mesorhizobia strains of A. senegal and A. seyal from 8 localities. Most of the strains (60%) clustered with the M. plurifarium type strain ORS 1032T, while the others form four new clades (MSP1 to MSP4). We sequenced and assembled seven draft genomes: four in the M. plurifarium clade (ORS3356, ORS3365, STM8773 and ORS1032T), one in MSP1 (STM8789), MSP2 (ORS3359) and MSP3 (ORS3324). The average nucleotide identities between these genomes together with the MLSA analysis reveal three new species of Mesorhizobium. A great variability of salt tolerance was found among the strains with a lack of correlation between the genetic diversity of mesorhizobia, their salt tolerance and the soils samples characteristics. A putative geographical pattern of A. senegal symbionts between the dryland north part and the center of Senegal was found, reflecting adaptations to specific local conditions such as the water regime. However, the presence of salt does not seem to be an important structuring factor of Mesorhizobium species.

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“…These OTUs were assigned to different genera within the Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes phyla. Studies based on cultivation carried out in Africa and Asia, which are considered to be the origin of cowpea diversity, reported only the presence of rhizobial strains assigned to the different genera within the Proteobacteria phylum in the nodules (Pule-Meulenberg et al, 2010; Krasova-Wade et al, 2014). Those strains were mainly classified as Bradyrhizobium sp., although Sarr et al (2009) also detected a strain of Ralstonia , a genus from the Betaproteobacteria class.…”

Section: Discussionmentioning

confidence: 99%

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

et al. 2017

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Many studies have been pointing to a high diversity of bacteria associated to legume root nodules. Even though most of these bacteria do not form nodules with legumes themselves, it was shown that they might enter infection threads when co-inoculated with rhizobial strains. The aim of this work was to describe the diversity of bacterial communities associated with cowpea (Vigna unguiculata L. Walp) root nodules using 16S rRNA gene amplicon sequencing, regarding the factors plant genotype and soil type. As expected, Bradyrhizobium was the most abundant genus of the detected genera. Furthermore, we found a high bacterial diversity associated to cowpea nodules; OTUs related to the genera Enterobacter, Chryseobacterium, Sphingobacterium, and unclassified Enterobacteriacea were the most abundant. The presence of these groups was significantly influenced by the soil type and, to a lesser extent, plant genotype. Interestingly, OTUs assigned to Chryseobacterium were highly abundant, particularly in samples obtained from an Ultisol soil. We confirmed their presence in root nodules and assessed their diversity using a target isolation approach. Though their functional role still needs to be addressed, we postulate that Chryseobacterium strains might help cowpea plant to cope with salt stress in semi-arid regions.

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Eco-geographical diversity of cowpea bradyrhizobia in Senegal is marked by dominance of two genetic types

Cited by 29 publications

References 50 publications

International Research Journal of Agriculture and Soil Science

International Research Journal of Agriculture and Soil Science

Genetic and Genomic Diversity Studies of Acacia Symbionts in Senegal Reveal New Species of Mesorhizobium with a Putative Geographical Pattern

Cowpea Nodules Harbor Non-rhizobial Bacterial Communities that Are Shaped by Soil Type Rather than Plant Genotype

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