ECM coating modification generated by optimized decellularization process improves functional behavior of BMSCs

Li, Mei; Zhang, Tingxia; Jiang, Jingxin; Mao, Yuxing; Zhang, Anqi; Zhao, Jiyuan

doi:10.1016/j.msec.2019.110039

Cited by 35 publications

(36 citation statements)

References 38 publications

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“…89 ECMs derived from adipose tissue or cartilage were also shown to induce MSC differentiation into adipocytes or chondrocytes, respectively. [90][91][92][93] However, the molecular mechanisms involved in the modulation of MSC behaviors on a particular decellularized matrix are still not well understood. For various tsECMs, collagen type I is the most characterized ECM protein preserved after decellularization and its abundance may contributes to the increased MSC proliferation on acellular ECMs.…”

Section: Mesenchymal Stem Cell Proliferation and Differentiation On Tmentioning

confidence: 99%

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

Sart

Jeske

Chen

et al. 2020

Tissue Engineering Part B: Reviews

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Stem cells, including mesenchymal stem cells and pluripotent stem cells, have attracted considerable attention in tissue engineering and regenerative medicine primarily because of their unique ability in self-renewal and multilineage differentiation. However, stem cells also have important secretory functions that form a specialized in vivo microenvironment and direct tissue development and regeneration. Extracellular matrices (ECMs) derived from stem cells retain the functional properties of their native environment and exhibit unique signaling that mediates stem cell self-renewal and lineage commitment. Stem cell-derived ECMs (scECMs) also have tunable properties corresponding to their developmental stages, suggesting that their lineage-and developmental specificity can be engineered for a wide range of applications. Hence, there is a growing interest in reconstructing stem cell microenvironment through decellularization and obtaining decellularized matrices that exhibit unique biological properties. This article summarizes recent advances in the use and understanding of scECMs. Moreover, future directions to extend the spectrum of applications of stem-derived ECMs in tissue engineering by comprehensively elucidating and engineering their regulatory function is highlighted.

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Section: Mesenchymal Stem Cell Proliferation and Differentiation On Tmentioning

confidence: 99%

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

Sart

Jeske

Chen

et al. 2020

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…They will loss of stem cell characteristics and functions after expansion in vitro [21,22]. Acellular matrix(ACM) plays an ideal three-dimensional(3D) platform for regenerative medicine because it is biocompatible and preserves 3D geometric and spatial architecture [23].…”

Section: Introductionmentioning

confidence: 99%

Pancreaticline Cell Differentiation of Bone Marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

Zhao

Wang

2020

Preprint

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Background: To evaluate the potential differentiation ability of bone mesenchymal stromal cells(BMSCs) to pancreatic line Cells on rat acellular pancreatic bioscaffold(APB) and the effect of differentiated BMSCs for chronic pancreatitis(CP) in vivo. Methods: After BMSCs were isolated and identified, they were dynamic cultured on the APB and static cultured in tissue culture flask(TCF),with or without the growth factors (GF) in both the culture system. The cytological behavior such as the proliferation and differentiation of BMSCs in all the above kinds of culture system were assessed by morphological observation, flow cytometry, ELASA analysis, qRT-PCR assay and western blot analysis. For the in vivo study, the pancreatic fibrosis and pathological score were evaluated. And also the expression of α-SMA, collagen type I and III, IL-10 in pancreas tissue were detected by ELASA. Results: 4ml/min was the most appropriate flow rate for the dynamic culture of BMSCs. The proliferation rate of BMSCs in the APB groups were significantly increased compared to TCF system. During the pancreatic line cell differentiation process, APB could induce BMSCs express markers such as PDX-1 and PTF-1 at higher mRNA levels. In contrast, the marker Oct 4 was expressed at a lower level in APB group. For the pancreatic functional cytoketatins including α-Amy, CK7, Flk-1, and C-peptide, they were all expressed at higher level in APB group. And metabolic enzymes secretion such as amylase and insulin were promoted significantly in APB system. By scanning electron microscope(SEM) and transmission electron microscopy(TEM), the ultrastructure of BMSCs in the APB group could further demonstrated the morphological characteristics of pancreatic-like cells. In vivo study,the expression of α-SMA, collagen type I and III in tissues were less in differentiated BMSCs treatment group, while the level of IL-10 in pancreatic tissue were higher in differentiated BMSCs treatment group with significant difference (P<0.05). In addition, in both in vitro and in vivo study, GF could significantly facilitate the function of proliferation, differentiation and pancreatic cell therapy. Conclusion: Together our data show the capacity of APB , 3D pancreatic biomatrix, promoting BMSCs differentiate toward pancreatic line phenotypes, and the considerable potential of using these cells for pancreatic cell therapies and tissue engineering.

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“…Previous study indicated that certain kinds whole organ ACM could support BMSCs differentiating into mature cells and expressing functional markers [26][27][28][29]. Furthermore, this type of induced cells had potential applications in regenerative therapy and tissue repair [30]. But less research previously have been shown about whole organ ACM promoting BMSCs pancreatic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Pancreatic line Cell Differentiation of bone marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

Zhao

Wang

2020

Preprint

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Background: To evaluate the potential differentiation ability of bone mesenchymal stromal cells(BMSCs) to pancreatic line Cells on rat acellular pancreatic bioscaffold (APB). Methods: Fresh pancreata from 20 Adult Sprague Dawley rats (between 6 and 7 weeks old) were soaked and perfused using Easy-Load Digital Drive peristaltic pumps. After BMSCs were isolated and identified, they were dynamic cultured on the APB and static cultured in tissue culture flask(TCF). Based on whether the differentiation was induced by the growth factors (GF) in the culture system, our study was divided into 4 groups: BMSCs cultured in TCF without any GF(TCF-GF(-)), BMSCs cultured in TCF with GF(TCF-GF(+)), BMSCs cultured on APB without GF(APB-GF(-)), BMSCs cultured on APB with GF (APB-GF(+)).The cytological behavior such as the proliferation and differentiation of BMSCs in all the above kinds of culture system with or without GF were assessed by morphological observation, flow cytometry, ELASA analysis, qRT-PCR assay and western blot analysis. Results: 4ml/min was the most appropriate flow rate for the dynamic culture of BMSCs. Under culture conditions, BMSCs populations could attach to and proliferate within the APB. APB could promote the proliferation and viability of BMSCs significantly better in dynamic culture with optimal flow rate 4ml/min, when compared to the static culture system. Also, the proliferation rate of BMSCs in the APB groups were significantly increased compared to TCF system. During the pancreatic line cell differentiation process, APB could induce BMSCs into pancreatic-like cells which expressed markers such as pancreatic duodenal homeodomain containing transcription factor (PDX-1) and pancreatic exocrine transcription factor (PTF-1) higher at mRNA levels compared to TCF system. In contrast, the marker Oct4 (octamer-binding transcription factor 4) was expressed at a lower level in APB group. For the pancreatic functional cytoketatins including α-Amylase(α-Amy), cytokeratin 7(CK7), fetal liver kinase-1 (Flk-1),and C-peptide, they were all expressed at higher level in APB group than in the TCF group. And metabolic enzymes secretion such as amylase and insulin were promoted significantly in APB system. By scanning electron microscope(SEM) and transmission electron microscopy(TEM), the ultrastructure of BMSCs in the APB group could further demonstrated the morphological characteristics of pancreatic-like cells. In addition, in both the dynamic and static system, GF could significantly facilitate the function of proliferation, differentiation and cell engraftment . Conclusion: Together our data show the capacity of APB , 3D pancreatic biomatrix, promoting BMSCs differentiate toward pancreatic line phenotypes, and the considerable potential of using these cells for pancreatic cell therapies and tissue engineering.

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ECM coating modification generated by optimized decellularization process improves functional behavior of BMSCs

Cited by 35 publications

References 38 publications

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

Pancreaticline Cell Differentiation of Bone Marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

Pancreatic line Cell Differentiation of bone marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

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