Echovirus 30 detection in an outbreak of acute myalgia and rhabdomyolysis, Brazil 2016–2017

Sousa, Ivanildo P.; Burlandy, Fernanda Marcicano; Lima, Stéphany Teixeira; Maximo, A.C.B.; Figueiredo, Manuel; Maia, Zuinara Pereira Gusmão; Silva, E.E. da

doi:10.1016/j.cmi.2018.08.018

Cited by 14 publications

(16 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, previous work have demonstrated that the co-circulation of a high number of different NPEV types, especially those from EV-C strains, favors recombination events [4][5] as shown by the recent isolation of type 2 VDPV polioviruses from the environment and the identification of an uncommon recombinant type3/type2 Sabinrelated poliovirus in Brazil [34,35]. Moreover, multiple infections by different NPEVs have also been associated with atypical clinical presentations [7,50].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)

Sousa

Oliveira

Burlandy

et al. 2020

Emerging Microbes & Infections

View full text Add to dashboard Cite

Due to the advanced stage of polio eradication, the possible role of non-polio enteroviruses (NPEVs) associated to acute flaccid paralysis (AFP) cases has been highlighted. In this study, we described epidemiological aspects of NPEVs infections associated to AFP and explore the viral genetic diversity, information still scarce in Brazil. From 2005 to 2017, 6707 stool samples were collected in the scope of the Brazilian Poliomyelitis Surveillance Program. NPEVs were isolated in 359 samples (5.3%) and 341 (94.9%) were genotyped. About 46 different NPEV types were identified with the following detection pattern EV-B > EV-A > EV-C. The major EV-types were CVA2, CV4, EV-A71, CVB3, CVB5, E6, E7, E11, CVA13 and EV-C99, which corresponds to 51.6% of the total. Uncommon types, such as CVA12, EV-90 and CVA11, were also identified. Different E6 genogroups were observed, prevailing the GenIII, despite periods of co-circulation, and replacement of genogroups along time. CVA2 sequences were classified as genotype C and data suggested its dispersion in South-American countries. CVA13 viruses belonged to cluster B and Venezuelan viruses composed a new putative cluster. This study provides extensive information on enterovirus diversity associated with AFP, reinforcing the need of tailoring current surveillance strategies to timely monitor emergence/re-emergence of NPEVs.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)

Sousa

Oliveira

Burlandy

et al. 2020

Emerging Microbes & Infections

View full text Add to dashboard Cite

show abstract

“…In general, 25% of the genome difference of enterovirus is used as the criteria for serotyping, while 15% of the genome difference is used as the criteria for genotyping; this is widely accepted for serotyping and genotyping of enterovirus, such as EV-A71, CV-A16, and CV-A6 (Brown et al, 1999;Oberste et al, 1999a,b;Mizuta et al, 2005;Zhang et al, 2010aZhang et al, , 2013Song et al, 2017). We refer to the published genotyping criteria of EV-A71, CV-A16, and CV-A6 and combined with the latest research progress of E-30 (Lema et al, 2019;Maruo et al, 2019;Mauri et al, 2019;Sousa et al, 2019). In addition to being able to better represent the E-30 sequences in the world, we also avoid the redundancy sequences of E-30 for genotyping, which leads to the confusion and divergence of enterovirus genotyping.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…The outbreaks of E-30, which were closely associated with encephalitis, were also frequently reported in other parts of the world, including Italy, Bulgaria, France, United Kingdom, Spain, Brazil, and Korea (Cabrerizo et al, 2008;Yang et al, 2013;Mladenova et al, 2014;Holmes et al, 2016). Surprisingly, a recent report showed that E-30 was detected in an outbreak of acute myalgia and rhabdomyolysis, indicating that E-30 possibly caused severe nonneuropathic diseases and that E-30 could cause a variety of clinical symptoms (Sousa et al, 2019).…”

Section: Introductionmentioning

confidence: 95%

“…There were several reports of encephalitis caused by E-30 worldwide (Wang et al, 2002;Cabrerizo et al, 2008;dos Santos et al, 2011;Kim et al, 2012;Milia et al, 2013), and the E-30 isolates were frequently detected from cases of encephalitis, indicating epidemiological relationship between E-30 and encephalitis. In rare cases, it can cause cute myalgia, rhabdomyolysis, and acute flaccid paralysis in immunosuppressed transplant recipients (Mauri et al, 2019;Sousa et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Large-Scale Outbreak of Echovirus 30 in Gansu Province of China in 2015 and Its Phylodynamic Characterization

Chen

Han

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Background: Echovirus 30 (E-30) has been investigated and reported worldwide and is closely associated with several infectious diseases, including encephalitis; myocarditis; and hand, foot, and mouth disease. Although many E-30 outbreaks associated with encephalitis have been reported around the world, it was not reported in northwest China until 2015. Methods: The clinical samples, including the feces, serum, throat swabs, and cerebrospinal fluid, were collected for this study and were analyzed for diagnosis. E-30 was isolated and processed according to the standard procedures. The epidemiological and phylogenetic analysis were performed to indicate the characteristics of E-30 outbreaks and phylodynamics of E-30 in China. Results: The E-30 outbreaks affected nine towns of Gansu Province in 2015, starting at a school of Nancha town and spreading to other towns within 1 month. The epidemiological features showed that children aged 6-15 years were more susceptible to E-30 infection. The genotypes B and C cocirculated in the world, whereas the latter dominated the circulation of E-30 in China. The genome sequences of this outbreak present 99.3-100% similarity among these strains, indicating a genetic-linked aggregate outbreak of E-30 in this study. Two larger genetic diversity expansions and three small fluctuations of E-30 were observed from 1987 to 2016 in China, which revealed the oscillating patterns of E-30 in China. In addition, the coastal provinces of China, such as Zhejiang, Fujian, and Shandong, were initially infected, followed by other parts of the country. The E-30 strains isolated from mainland of China may have originated from Taiwan of China in the last century. Conclusion: The highly similar E-30 genomes in this outbreak showed an aggregate outbreak of E-30, with nine towns affected. Our results suggested that, although the genetic diversity of E-30 oscillates, the dominant lineages of E-30 in China has complex

show abstract

“…Human enteroviruses (EV) are small non-enveloped RNA viruses belonging to the picornaviridae family and are classified into four species: EV-A, B, C, and D. Although most EV infections can be asymptomatic, they are associated with a broad spectrum of clinical presentations such as hand, foot, and mouth disease, acute myalgia, herpangina, conjunctivitis, upper and lower respiratory disease and severe syndromes of the central nervous system (CNS) [1,2]. Among the most common manifestations involving the CNS, it is worth mentioning encephalitis, acute flaccid paralysis, paralytic myelitis and aseptic meningitis [1].…”

Section: Introductionmentioning

confidence: 99%

Identification and Phylogenetic Characterization of Human Enteroviruses Isolated from Cases of Aseptic Meningitis in Brazil, 2013–2017

Ramalho

Sousa

Burlandy

et al. 2019

Viruses

View full text Add to dashboard Cite

Aseptic meningitis is a common viral infection associated with human enteroviruses. The aim of the present study was to identify and characterize the enteroviruses associated with outbreaks and sporadic cases of aseptic meningitis that occurred in different regions of Brazil between 2013 and 2017. Cerebrospinal fluids obtained from patients admitted to public health facilities were analyzed. A total of 303 patients were positive for Human Enteroviruses (EV) by cell culture isolation with a median isolation rate throughout the year of 12%. We were able to identify enterovirus serotypes in 295 clinical specimens. Nineteen different serotypes were identified; the large majority corresponded to HEV-B species. Echovirus 30 (E-30) and Echovirus 6 (E-6) were the most prevalent genotypes (66.8%). Sequence analysis suggested that circulating E-30 was closely related to E-30 from other American countries; while E-6 was derived from Europe. Most of the patients consisted of children ≤ 15 years old. The temporal distribution of all aseptic meningitis and EV-positive cases showed an obvious seasonal pattern during autumn. Our results have provided valuable information about the enteroviral etiology of the aseptic meningitis cases in Brazil pointing to the importance of enterovirus surveillance in neurological diseases.

show abstract

Echovirus 30 detection in an outbreak of acute myalgia and rhabdomyolysis, Brazil 2016–2017

Abstract: The 2016-2017 outbreak of acute myalgia that occurred in the northeast region of Brazil can be associated with E-30. Despite the clinical manifestations, a favourable outcome was observed for all patients.

Cited by 14 publications

References 13 publications

Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)

Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)

A Large-Scale Outbreak of Echovirus 30 in Gansu Province of China in 2015 and Its Phylodynamic Characterization

Identification and Phylogenetic Characterization of Human Enteroviruses Isolated from Cases of Aseptic Meningitis in Brazil, 2013–2017

Contact Info

Product

Resources

About