Echis ocellatus Venom-Induced Reproductive Pathologies in Rat Model; Roles of Oxidative Stress and Pro-Inflammatory Cytokines

Ajisebiola, Babafemi Siji; Alamu, Priscilla Ifeoluwa; James, Adewale Segun; Adeyi, Akindele Oluwatosin

doi:10.3390/toxins14060378

Cited by 4 publications

(1 citation statement)

References 51 publications

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“…A: vacuolization around the nucleus of spermatocytes (blue arrows); A, B: a small number of irregularly placed spermatogonia (yellow arrows); A, D: Leydig cells in the interstitium (red arrows); B, C: erythrocytes within the amorphous mass in the lumen of seminiferous tubules (green ovals); C, D: cell mass from spermatogenic cells of various degrees of maturity with blurred edges (yellow ovals). Staining with hematoxylin and eosin; x 1000. changes in the germinal epithelium and the epithelium of seminiferous tubules, and tubular atrophy [2].…”

Section: Discussionmentioning

confidence: 99%

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

Niyazmetov

2023

Rep. of Morph.

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Morpho-functional changes in the organs of the male reproductive system are usually associated with injuries, infectious diseases, age, lifestyle, the presence of bad habits (abuse of alcohol, drugs), the influence of environmental factors, etc. However, the action of natural toxins, in particular those that are components of animal venoms, including the venoms of snakes and vipers, is no less important. The aim of the research is to study the features of the microscopic structure of the testis of rats under the influence of Vipera berus berus venom. Experimental studies were carried out on white non-linear male rats. Animals were conditionally divided into two groups – control and experimental, 10 individuals in each. Experimental rats were injected intraperitoneally with a semi-lethal dose (LD50) (1.576 mg/g-1) of Vipera berus berus venom in physiological solution. Animals of the control group were injected intraperitoneally with only saline solution. Rats were removed from the experiment 24 hours after exposure of the venom, anesthetized by cervical dislocation. Testis samples were taken for microscopic examination. Fixation of the material and preparation of paraffin blocks were carried out according to generally accepted methods. Staining of histological preparations of the testis was carried out with hematoxylin and eosin. Histological preparations were studied using a SEO SСAN light microscope. Administration of Vipera berus berus venom to rats leads to the development of pathogistological changes in the parenchymal elements of the testis of animals, among which desquamation and disorganization of all stages of spermatogenic cells development, disruption of spermatogenesis processes were the most pronounced. Spermatogenic cells of the seminiferous tubules of the testis were distinguished by changes in morphology and location, lost the regularity of their placement, and their number decreased. The cells detached from the basement membrane and did not form a continuous layer. A characteristic feature was the presence of clusters of erythrocytes in the lumen of the seminiferous tubules, which may indicate the development of hemorrhagic complications. In addition, an increase in the number of Leydig cells was detected, which is probably the cause of changes in the hormonal regulation of the organ's function.

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Section: Discussionmentioning

confidence: 99%

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

Niyazmetov

2023

Rep. of Morph.

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An in vivo assessment of inflammatory and oxidative stress responses in Echis ocellatus-venom induced cardiotoxicity

et al. 2022

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Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats

Adeyi,

Ajisebiola,

Sanni

et al. 2024

Sci Rep

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Naja nigricollis Venom (NnV) contains complex toxins that affects various vital systems functions after envenoming. The venom toxins have been reported to induce male reproductive disorders in envenomed rats. This present study explored the ameliorative potential of kaempferol on NnV-induced male reproductive toxicity. Fifty male wistar rats were sorted randomly into five groups (n = 10) for this study. Group 1 were noted as the control, while rats in groups 2 to 5 were injected with LD50 of NnV (1.0 mg/kg bw; i.p.). Group 2 was left untreated post envenomation while group 3 was treated with 0.2 ml of polyvalent antivenom. Groups 4 and 5 were treated with 4 and 8 mg/kg of kaempferol, respectively. NnV caused substantial reduction in concentrations of follicle stimulating hormone, testosterone and luteinizing hormone, while sperm motility, volume and counts significantly (p < 0.05) decreased in envenomed untreated rats. The venom enhanced malondialdehyde levels and substantially decreased glutathione levels, superoxide dismutase and glutathione peroxidase activities in the testes and epididymis of envenomed untreated rats. Additionally, epididymal and testicular myeloperoxidase activity and nitric oxide levels were elevated which substantiated severe morphological defects noticed in the reproductive organs. However, treatment of envenomed rats with kaempferol normalized the reproductive hormones with significant improvement on sperm functional parameters. Elevated inflammatory and oxidative stress biomarkers in testis and epididymis were suppressed post kaempferol treatment. Severe histopathological lesions in the epididymal and testicular tissues were ameliorated in the envenomed treated groups. Results highlights the significance of kaempferol in mitigating reproductive toxicity induced after snakebite envenoming.

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Echis ocellatus Venom-Induced Reproductive Pathologies in Rat Model; Roles of Oxidative Stress and Pro-Inflammatory Cytokines

Cited by 4 publications

References 51 publications

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

Peculiarities of the microscopic structure of rat testis under the influence of Vipera berus berus venom

An in vivo assessment of inflammatory and oxidative stress responses in Echis ocellatus-venom induced cardiotoxicity

Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats

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