Echinostoma ` revolutum ¿ (Digenea: Echinostomatidae) species complex revisited: species delimitation based on novel molecular and morphological data gathered in Europe

Georgieva, Simona; Faltýnková, Anna; Brown, Rebecca; Blasco‐Costa, Isabel; Soldánová, Miroslava; Sitko, Jiljí; Scholz, T.; Kostadinova, Aneta

doi:10.1186/preaccept-1112350941442199

Cited by 19 publications

(101 citation statements)

References 46 publications

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“…In concurrence with previous studies (Morgan and Blair 1998;Georgieva et al 2014), the ND1 region proved to be more informative than the SSU, LSU, ITS1/5.8S/ITS2, or CO1 regions. The rRNA region of the D. auritus isolate from Brazil and USA shared 99.9 % identity across 4267 nt.…”

Section: Discussionsupporting

confidence: 91%

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Pinto

Griffin

Quiniou³

et al. 2015

Parasitol Res

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Species of trematodes belonging to the genus Drepanocephalus are intestinal parasites of piscivorous birds, primarily cormorants (Phalachrocorax spp.), and are widely reported in the Americas. During a 4-year malacological study conducted on an urban lake in Brazil, 27-collar-spined echinostome cercariae were found in 1665/15,459 (10.7 %) specimens of Biomphalaria straminea collected. The cercariae were identified as Drepanocephalus spp. by sequencing the 18S (SSU) rDNA, ITS1/5.8S rDNA/ITS2 (ITS), 28S (LSU) rDNA region, cytochrome oxidase subunit 1 (CO1), and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) markers. In experimental life cycle studies, metacercariae developed in laboratory-reared guppies (Poecilia reticulata); however, attempts to infect birds and rodents were unsuccessful. Two closely related morphotypes of cercariae were characterized. One species, identified by molecular markers as a genetic variant of Drepanocephalus auritus (99.9 % similarity at SSU, ITS, LSU; 97.2 % at CO1; 95.8 % at ND1), differs slightly from an archived North American isolate of this species also sequenced as part of this study. A second species, putatively identified as Drepanocephalus sp., has smaller cercariae and demonstrates significant differences from D. auritus at the CO1 (11.0 %) and ND1 (13.6 %) markers. Aspects related to the morphological taxonomic identification of 27-collar-spined echinostome metacercariae are briefly discussed. This is the first report of the involvement of molluscs of the genus Biomphalaria in the transmission of Drepanocephalus and the first report of D. auritus in South America.

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Section: Discussionsupporting

confidence: 91%

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Pinto

Griffin

Quiniou³

et al. 2015

Parasitol Res

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“…Considering that Cotylurus spp. have been described as having snails as a second‐intermediate host, it is possible for them to use the same species, although typically not the exact same snail individual (Graczyk & Shiff, ). Meaning that further sampling of S. elodes may uncover metacercariae of C. marcogliesei .…”

Section: Discussionmentioning

confidence: 99%

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Gordy

Hanington

2019

Ecology and Evolution

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Despite over 100 years of digenean trematode parasite species descriptions, from a wide diversity of vertebrate and invertebrate host species, our ability to recognize the diversity of trematode species within a single lake remains an incredible challenge. The most challenging aspect is the identification of species from larval stages derived from intermediate hosts, due to the disjointed data of adult worm morphological descriptions, from which species are named, and links to corresponding molecular identifiers in depauperate databases. Cryptic species also play a significant role in the challenge of linking trematode larvae to adults, species identifications, and estimating diversity. Herein, we utilize a large, longitudinal dataset of snail first‐intermediate host infection data from lakes in Alberta, Canada, to infer trematode larval diversity using molecular phylogenetics and snail host associations. From our assessments, we uncover a diversity of 79 larval trematode species among just five snail host species. Only 14 species were identified to a previously described species, while the other 65 species are either cryptic or otherwise unrepresented by mitochondrial genes in GenBank. This study currently represents the largest and most diverse singular molecular survey of trematode larval fauna composed of over one thousand mitochondrial sequences. Surprisingly, rarefaction analyses indicate we have yet to capture the complete diversity of trematodes from our sampling area.

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“…This framework emphasises an integrative approach to taxonomy (Dayrat, 2005;Will et al, 2005) rather than one based on singular criteria, and has already been successfully applied to recognise many trematode species (e.g. Georgieva et al, 2013, Razo-Mendivil et al, 2013Georgieva et al, 2014 in association with Faltýnková et al, 2015;Blasco-Costa et al, 2014 in association with Faltýnková et al, 2014;Herrmann et al, 2014;Diaz et al, 2015). This approach is not completely new to parasite taxonomists, since ecological data (e.g.…”

Section: Species Concepts Applied To Trematodes In the Molecular Agementioning

confidence: 99%

“…This issue is pertinent even if morphological characterisation is not the goal of the study (e.g. barcoding or molecular ecology studies), since GenBank is plagued by misidentified sequences (Valkiūnas et al, 2008;Georgieva et al, 2014). The scientific community is just starting to comprehend the importance of vouchering molecular samples.…”

Section: Voucher Specimensmentioning

confidence: 99%

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Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

et al. 2016

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'Molecular approaches to trematode systematics: 'best practice' and implications for future study ', Systematic Parasitology, 93(3), 295-306. The final publication is available at Springer via http://dx.doi.org/10.1007/s11230-016-9631-2.The full details of the published version of the article are as follows: However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the 'best molecular practice' for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work.Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient 'useful' comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics.

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Echinostoma ` revolutum ¿ (Digenea: Echinostomatidae) species complex revisited: species delimitation based on novel molecular and morphological data gathered in Europe

Cited by 19 publications

References 46 publications

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

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