Echinops spinosissimus Turra Root Methanolic Extract: Characterization of the Bioactive Components and Relative Wound Healing, Antimicrobial and Antioxidant Properties

Zitouni-Nourine, Saida Hanane; Belyagoubi-Benhammou, Nabila; Zitouni-Haouar, Fatima El-Houaria; Douahi, Omar; Chenafi, Faouzia; Fetati, H.; Sari, Siham Chabane; Benmahieddine, Assia; Zaoui, Chahinez; Mekaouche, F.Z.N.; Bekkara, Fawzia Atik; Kambouche, Nadia; Gismondi, Angelo; Toumi, Houari

doi:10.3390/plants11243440

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Plants have long been used as food, ornaments, and natural preservatives against the development of microorganisms and for the treatment of diseases [7]. Previous studies have shown that plant extracts are rich in certain substances called secondary metabolites, which give them antibacterial capacity [8].…”

Section: Introductionmentioning

confidence: 99%

Resistance Profile of Bacteria Isolated from Diabetic Wounds: Phytochemicals and Antibacterial Studies of Eriosema robustum Leaf Extracts

Tsaffo,

Djenguemtar,

Fodouop

et al. 2024

Trends Sci

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Diabetics are susceptible to severe trophic problems and wounds, which are frequently referred to as “unhealable wounds”. Untreated diabetic wounds provide an ideal habitat for the growth of bacteria, which can eventually develop resistance to standard treatments and need amputation. This study aimed to assess the in vitro antibacterial activity of ethanolic and aqueous extracts of Eriosema robustum leaves on the same bacterium, as well as the resistance profile of bacteria isolated from diabetic wounds to conventional antibiotics. Thirty samples from diabetic wounds were collected at the Baleveng Hospital, subjected to bacteria isolation and antibiotics resistance tests using the disk diffusion method. On the other hands, leaves of Eriosema robustum were collected, hydroethanolic extraction were performed. The extract obtained was used for the antibacterial (by determination of the MIC) and antioxidant (DPPH and FRAP tests) activities as well as phytochemicals composition (using HPLC methods) assessment. Amon the 30 samples analyzed, 19 (63.33 %) showed bacterial infections and Staphylococcus aureus was the most common pathogen (48 %), followed by Pseudomonas aeruginosa (24 %), Klebsiella pneumoniae (19 %), and Streptococcus agalactiae (9 %). Fifty percent (50 %) of S. aureus isolates were MRSA (methicillin-resistant S. aureus). The MIC value obtained 64 μg/mL against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. Phytochemicals analysis of fractions from the 70 ° hydroethanolic leaves of Eriosema robustum revealed the presence of coumarin, gallic acid, quinoline, vanillin, ascorbic acid, caffeic acid, kaempferol, and cinnamic acid. The antibacterial and antioxidant potential of the extracts highlighted in this study could make this plant a good avenue for the discovery of new molecules effective against infected diabetic wounds. HIGHLIGHTS In this study, a total of 30 diabetic wounds were examined, and it was found that 63.33 % of them had infections. Staphylococcus aureus was the most frequently occurring bacteria, followed by Pseudomonas aeruginosa, Klebsiella pneumoniae, and Streptococcus agalactiae. The current study has revealed that, 96 % of the Staphylococcus aureus strains isolated were resistant to both tetracycline and methicillin, with the majority of the bacteria also showing resistance to amoxicillin. The hydroethanolic and aqueous extracts of Eriosema robustum displayed antibacterial activity against most of the isolated bacteria and the phytochemical analysis (HPLC) of fractions from the 70 ° hydroethanolic leaf extract revealed the presence of several secondary metabolites, including coumarin, gallic acid, quinoline, vanillin, ascorbic acid, caffeic acid, kaempferol, and cinnamic acid. This study opens up a new horizon in the management of diabetic wounds, and makes Eriosema robustum a potential candidate for the formulation of new drugs active against multi-resistant bacteria found in diabetic subjects. GRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Resistance Profile of Bacteria Isolated from Diabetic Wounds: Phytochemicals and Antibacterial Studies of Eriosema robustum Leaf Extracts

Tsaffo,

Djenguemtar,

Fodouop

et al. 2024

Trends Sci

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Echinops Asteraceae extract guards against malathion-induced liver damage via minimizing oxidative stress, inflammation, and apoptosis

Eid,

Abadi,

Alghamdi

et al. 2024

Toxicon

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Morphological Features and Biological Activity of Different Extracts of Echinops spinosissimus Grown in Saudi Arabia

Masoudi

Hashim

2023

Agronomy

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Based on a shortage of available data on Echinops spinosissimus in Saudi Arabia, the current study’s aim was to present some new information on the topic. Plant samples were collected from different locations in the northeast of Mecca. Out of fifteen species from this genus found in Saudi Arabia, one species was targeted in the current study. It was noted as a perennial subshrub that is 30–80 cm in length. Its stem is gray, striate, and slightly covered with glandular hairs. The epidermis is converted into cork cells in older stem parts. The vascular system showed a continuous siphonostelic structure and dissected vascular bundles. The lamina is abaxially rounded and straight. The pollen grains are monads, radially symmetric, medium-sized, and a prolate spheroidal shape with an aculeate–foveolate exine structure. Based on its historical pharmaceutical properties, the phytochemical properties were studied, and it was noted that ethyl acetate was the best solvent for producing high amounts of bioactive compounds such as phenols, flavonoids, and alkaloids. The obtained extracts appeared to exhibit high activity against Gram-positive pathogenic bacteria. These extracts were identified by using HPLC and GC-MS. Many bioactive compounds were detected, such as protocatechuic acid, gallic acid, rutin, vanillic acid, quercetin, and kaempferol. Additionally, four main compounds, including hexadecanoic, stearic, oleic, and linoleic acids, were detected via GC-MS. The total antioxidants of E. spinosissimus extracts showed that the ethyl acetate extract exhibited a high total antioxidant capacity and free radical-scavenging properties.

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Echinops spinosissimus Turra Root Methanolic Extract: Characterization of the Bioactive Components and Relative Wound Healing, Antimicrobial and Antioxidant Properties

Cited by 3 publications

References 53 publications

Resistance Profile of Bacteria Isolated from Diabetic Wounds: Phytochemicals and Antibacterial Studies of Eriosema robustum Leaf Extracts

Resistance Profile of Bacteria Isolated from Diabetic Wounds: Phytochemicals and Antibacterial Studies of Eriosema robustum Leaf Extracts

Echinops Asteraceae extract guards against malathion-induced liver damage via minimizing oxidative stress, inflammation, and apoptosis

Morphological Features and Biological Activity of Different Extracts of Echinops spinosissimus Grown in Saudi Arabia

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