Echinoid facilitates Notch pathway signalling during<i>Drosophila</i>neurogenesis through functional interaction with Delta

Rawlins, Emma L.; Lovegrove, Bridget; Jarman, Andrew P.

doi:10.1242/dev.00882

Cited by 26 publications

(26 citation statements)

References 48 publications

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“…Although the level of Egfr is also upregulated in cells more posterior to the MF, these levels might not be high enough to cause sustained MAPK activation. In the wing disc, Ed also facilitates Notch signaling to promote mesothorax bristle patterning (Ahmed et al, 2003;Escudero et al, 2003;Rawlins et al, 2003b). In fact, Ed colocalizes with Notch/Delta in Hrs-containing early endosomes (Escudero et al, 2003;Rawlins et al, 2003b).…”

Section: Research Articlementioning

confidence: 99%

“…In the wing disc, Ed also facilitates Notch signaling to promote mesothorax bristle patterning (Ahmed et al, 2003;Escudero et al, 2003;Rawlins et al, 2003b). In fact, Ed colocalizes with Notch/Delta in Hrs-containing early endosomes (Escudero et al, 2003;Rawlins et al, 2003b). However, it remains unclear whether the endocytosis of Ed plays any role in facilitating Notch signaling in the wing discs.…”

Section: Research Articlementioning

confidence: 99%

“…Both Ed and Fmi have been shown to be present in the endocytic compartment, indicating that they are endocytosed (Rawlins et al, 2003b;Rawls and Wolff, 2003;Strutt and Strutt, 2008). During endocytosis, adaptors, such as the AP-2 complex, function to specifically recognize cargo receptors and to stimulate clathrin assembly (Robinson, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Echinoid regulates Flamingo endocytosis to control ommatidial rotation in theDrosophilaeye

Lien

Lin

et al. 2010

Development

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SUMMARYPlanar cell polarity (PCP) refers to a second polarity axis orthogonal to the apicobasal axis in the plane of the epithelium. The molecular link between apicobasal polarity and PCP is largely unknown. During Drosophila eye development, differentiated photoreceptors form clusters that rotate independently of the surrounding interommatidial cells (ICs). Here, we demonstrate that both Echinoid (Ed), an adherens junction-associated cell adhesion molecule, and Flamingo (Fmi), a PCP determinant, are endocytosed via a clathrin-mediated pathway in ICs. Interestingly, we found that Ed binds the AP-2 adaptor and is required for the internalization of Fmi into ICs. Loss of ed led to increased amounts of Fmi on the cell membrane of non-rotating ICs and also to the misrotation of photoreceptor clusters. Importantly, overexpression of fmi in ICs alone was sufficient to cause misrotation of the adjacent photoreceptor clusters. Together, we propose that Ed, when internalized by AP-2, undergoes co-endocytosis with, and thereby decreases, Fmi levels on non-rotating ICs to permit correct rotation of ommatidial clusters. Thus, co-endocytosis of Ed and Fmi provides a link between apicobasal polarity and PCP.

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Section: Research Articlementioning

confidence: 99%

Section: Research Articlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Echinoid regulates Flamingo endocytosis to control ommatidial rotation in theDrosophilaeye

Lien

Lin

et al. 2010

Development

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“…Echinoid protein (Ed) localizes to the adherens junction in wing imaginal disc cells, can bind to Baz, and appears to act as a cell adhesion molecule (Wei et al, 2005). Ed has also been linked to Notch signaling (Ahmed et al, 2003;Escudero et al, 2003;Rawlins et al, 2003). ed mutant clones in the wing are ringed by an F-actin cable that is also associated with elevated staining for Myosin II and decreased staining for Baz (Wei et al, 2005).…”

Section: Accumulation Of Myosin II At the D-v Compartment Boundarymentioning

confidence: 99%

Localization and requirement for Myosin II at the dorsal‐ventral compartment boundary of the Drosophila wing

Major

Irvine

2006

Developmental Dynamics

123

134

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As organisms develop, their tissues can become separated into distinct cell populations through the establishment of compartment boundaries. Compartment boundaries have been discovered in a wide variety of tissues, but in many cases the molecular mechanisms that separate cells remain poorly understood. In the Drosophila wing, a stripe of Notch activation maintains the dorsal-ventral compartment boundary, through a process that depends on the actin cytoskeleton. Here, we show that the dorsal-ventral boundary exhibits a distinct accumulation of Myosin II, and that this accumulation is regulated downstream of Notch signaling. Conversely, the dorsal-ventral boundary is depleted for the Par-3 homologue Bazooka. We further show that mutations in the Myosin heavy chain subunit encoded by zipper can impair dorsal-ventral compartmentalization without affecting anterior-posterior compartmentalization. These observations identify a distinct accumulation and requirement for Myosin activity in dorsal-ventral compartmentalization, and suggest a novel mechanism in which contractile tension along an F-actin cable at the compartment boundary contributes to compartmentalization. Developmental Dynamics 235: 3051-3058, 2006.

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“…Given the proposed role of Ed as a homophilic adhesion molecule (Islam et al, 2003;Rawlins et al, 2003a;Spencer and Cagan, 2003), selective affinity may also contribute to morphogenesis. For example, as the anterior and medial floor cells elongate towards the midline of the primordium, preferential affinity for the opposing floor cells, which also express Ed, over the roof cells, which lack Ed, may favor floor cell association.…”

Section: Multiple Forces Contribute To Morphogenesismentioning

confidence: 99%

Differential expression of the adhesion molecule Echinoid drives epithelial morphogenesis inDrosophila

2006

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Epithelial morphogenesis requires cell movements and cell shape changes coordinated by modulation of the actin cytoskeleton. We identify a role for Echinoid (Ed), an immunoglobulin domain-containing cell-adhesion molecule, in the generation of a contractile actomyosin cable required for epithelial morphogenesis in both the Drosophila ovarian follicular epithelium and embryo. Analysis of ed mutant follicle cell clones indicates that the juxtaposition of wild-type and ed mutant cells is sufficient to trigger actomyosin cable formation. Moreover, in wild-type ovaries and embryos, specific epithelial domains lack detectable Ed, thus creating endogenous interfaces between cells with and without Ed; these interfaces display the same contractile characteristics as the ectopic Ed expression borders generated by ed mutant clones. In the ovary, such an interface lies between the two cell types of the dorsal appendage primordia. In the embryo, Ed is absent from the amnioserosa during dorsal closure, generating an Ed expression border with the lateral epidermis that coincides with the actomyosin cable present at this interface. In both cases, ed mutant epithelia exhibit loss of this contractile structure and subsequent defects in morphogenesis. We propose that local modulation of the cytoskeleton at Ed expression borders may represent a general mechanism for promoting epithelial morphogenesis.

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Echinoid facilitates Notch pathway signalling duringDrosophilaneurogenesis through functional interaction with Delta

Cited by 26 publications

References 48 publications

Echinoid regulates Flamingo endocytosis to control ommatidial rotation in theDrosophilaeye

Echinoid regulates Flamingo endocytosis to control ommatidial rotation in theDrosophilaeye

Localization and requirement for Myosin II at the dorsal‐ventral compartment boundary of the Drosophila wing

Differential expression of the adhesion molecule Echinoid drives epithelial morphogenesis inDrosophila

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