Echinoderms from Azores islands: An unexpected source of antibiotic resistant Enterococcus spp. and Escherichia coli isolates

Marinho, Catarina; Silva, Nuno; Pombo, Sofia; Santos, Tiago; Monteiro, Ricardo; Gonçalves, Alexandre; Micael, Joana; Rodrigues, Pedro; Costa, Ana C.; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1016/j.marpolbul.2013.01.017

Cited by 27 publications

(20 citation statements)

References 39 publications

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“…In this study, the MAR E. faecalis isolate was found to harbour the tet(M) gene. This is consistent with previous research which reported that the tet(M) gene was frequently associated with tetracycline resistance in enterococci isolates (Marinho et al, 2013;Prichula et al, 2016). It is possible that Enterococcus spp.…”

Section: Treatment and Discussionsupporting

confidence: 93%

“…It is possible that Enterococcus spp. might act as reservoirs of antimicrobial resistant genes and could therefore spread resistance to other pathogenic bacteria in the marine environment (Marinho et al, 2013). In conclusion, the detection of MAR E. faecalis is an important finding.…”

Section: Treatment and Discussionmentioning

confidence: 94%

“…The presence of bacteria that is resistant to antibiotics in the marine environment can be used as an indicator of marine pollution (Marinho et al, 2013;Meena et al, 2015). The types of animals that are affected by bacterial antibiotic resistance include cows, goats, canine, seabirds, sea turtles, and marine mammals (Banik et al, 2016;Prichula et al, 2016;Swetha et al, 2017;Hristov, 2018;Robaj et al, 2018).…”

mentioning

confidence: 99%

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Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report

Tsai

Chang

2019

IJAR

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There has recently been an alarming increase in the prevalence of antibiotic resistant bacteria in marine environments. In this study, an Enterococcus sp. was isolated from bacteriologic cultures of the elbow joint of an olive ridley turtle (Lepidochelys olivacea ). The enterococci isolate was identified at the species level as Enterococcus faecalis using PCR and the RAPID ID 32 STREP system. Analysis of the antimicrobial resistance patterns and the antibiotic-resistance genes of the E. faecalis isolated in this study revealed multiple-antibiotic-resistance (MAR). Specifically, the isolate presented resistance to doxycycline, enrofloxacin, erythromycin, neomycin, oxytetracycline, gentamicin, amikacin, ciprofloxacin, spiramycin, chloramphenicol, ceftiofur, and azithromycin. We also identified the tetracycline resistance gene tet(M) in the E. faecalis isolate. Further research into the antimicrobial resistance patterns of Enterococcus spp. should be conducted to promote the conservation of sea turtles.

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Section: Treatment and Discussionsupporting

confidence: 93%

Section: Treatment and Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

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Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report

Tsai

Chang

2019

IJAR

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“…In Portugal, concerning wild animals, E. faecium were found to be the main species in seagulls (Radhouani et al, 2011b); birds of prey (Radhouani et al, 2012b), partridges (Silva et al, 2011b), Iberian wolf (Gonçalves et al, 2013a), red foxes (Radhouani et al, 2013), wild boars (Poeta et al, 2007a), gilthead seabream (Barros et al, 2011), and Echinoderms (Marinho et al, 2013), while E. faecalis was dominant in Passeriformes (Santos et al, 2013) and wild rabbits (Silva et al, 2010). On the other hand, E. hirae was the predominant specie isolated from Iberian lynx (Gonçalves et al, 2013b,c; Figure 2 ).…”

Section: Phylogenetic History and Genetic Structurementioning

confidence: 99%

Potential impact of antimicrobial resistance in wildlife, environment and human health

Radhouani¹,

Silva²,

Poeta³

et al. 2014

Front. Microbiol.

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181

135

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Given the significant spatial and temporal heterogeneity in antimicrobial resistance distribution and the factors that affect its evolution, dissemination, and persistence, it is important to highlight that antimicrobial resistance must be viewed as an ecological problem. Monitoring the resistance prevalence of indicator bacteria such as Escherichia coli and enterococci in wild animals makes it possible to show that wildlife has the potential to serve as an environmental reservoir and melting pot of bacterial resistance. These researchers address the issue of antimicrobial-resistant microorganism proliferation in the environment and the related potential human health and environmental impact.

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“…These studies do not give appraisal of the presence and types of tet genes that are present in commensal bacteria in the environment. Recent studies have shown that antimicrobial-resistant bacteria are present in different wild animals that have not been exposed to antibiotics [6,7]. Moreover, the resistance genes of bacteria from wild birds may result in increased numbers of resistant bacteria in livestock and humans [8].…”

Section: Introductionmentioning

confidence: 99%

Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens

Balasubramaniam¹,

Eswaran²,

Suresh³

et al. 2014

Vet World

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Aim:The aim was to study the occurrence of tetracycline resistance determinant tetA gene, and antibacterial resistance pattern in commensal Escherichia coli recovered from healthy non-clinical layer chickens. Materials and Methods:Twenty-four cloacal swabs were obtained from 15 flocks in five different layer farms located at around Namakkal, which is a place of high-intensity layer chicken rearing in south India. Identification of E. coli was carried out by performing cultural and biochemical tests. Antimicrobial resistance test was carried out using disc diffusion method. The polymerase chain reaction employing tetAC forward and tetAC reverse primers were carried out to detect tetA gene conferring resistance to tetracycline.Result: All the collected cloacal swabs yielded E. coli. Twenty-one isolates (88%) were resistant to tetracycline antibiotic in disc diffusion method. All the isolates showed resistance to more than six antibiotics, which implied existence of multidrug resistant microbiota in intestine of poultry. Only seven (29%) isolates showed the presence of tetA gene indicating the involvement of either other one or more efflux gene(s), namely tetB, tetC and tetD or ribosomal protection encoded by tetM, tetO, tetQ and tetS genes than tetA gene. Conclusion:Based on the presence of tetA gene among tetracycline-resistant bacteria in healthy non-clinical food-producing animals such as layer chickens, it can be significant in human medicine as tetA gene could easily be spread to other bacteria. This kind of phenomenon can be extrapolated in transfer of resistance for other antibacterial essential for treating bacterial infections in human. We conclude that preventing the spread of antimicrobial resistance through direct or indirect contact, consumed food/feed and through the environment is empirical in reduction of failures while treating bacterial infections.

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Echinoderms from Azores islands: An unexpected source of antibiotic resistant Enterococcus spp. and Escherichia coli isolates

Cited by 27 publications

References 39 publications

Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report

Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report

Potential impact of antimicrobial resistance in wildlife, environment and human health

Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens

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