Echinoderms

Harms, Craig A.

doi:10.1002/9780470960806.ch20

Cited by 1 publication

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coelomic fluid in healthy echinoderms is typically sterile (Bang and Lemma ; Jangoux ); therefore, the presence of microorganisms in coelomic fluid may represent bacteremia, sample contamination from the surface of the animal, or unintentional puncture of internal organs. Bacterial colonization of coelomic fluid can manifest as a result of poor water quality or physical trauma in echinoderms (Bang and Lemma ; Gilles and Pearse ; Harms ). In previous studies coelomic fluid was typically sterile in animals that were optimally managed (Unkles ), which is similar in the current study to group A, which had the lower prevalence of bacterial positive samples and were managed with a shorter holding time and lower water temperature.…”

Section: Discussionmentioning

confidence: 99%

“…, ; Lessios ; Hughes ). Etiologic agents responsible for the mass mortality events have been proposed; however, definitive identification is lacking (Bauer and Agerter ; Harms ; Clemente et al. ).…”

mentioning

confidence: 99%

“…; Clemente et al. ) and in clinical settings such as public aquariums (Harms ). Macro‐ and microscopic evaluation of wet‐mounts or bacterial cultures can be utilized to identify microorganisms in coelomic and fecal samples (Harms ).…”

mentioning

confidence: 99%

“…) and in clinical settings such as public aquariums (Harms ). Macro‐ and microscopic evaluation of wet‐mounts or bacterial cultures can be utilized to identify microorganisms in coelomic and fecal samples (Harms ). Coelomic fluid analysis is an important diagnostic tool in echinoderms and has been employed to identify bacteremia, quantify total protein concentration, and conduct amoebocyte counts (Bang and Lemma ; Jellett et al.…”

mentioning

confidence: 99%

“…Coelomic fluid analysis is an important diagnostic tool in echinoderms and has been employed to identify bacteremia, quantify total protein concentration, and conduct amoebocyte counts (Bang and Lemma ; Jellett et al. ; Harms ). In healthy echinoderms that are properly maintained, coelomic fluid is typically sterile and free of microorganisms due to effective pathogen defense mechanisms and immune responses, such as coelomocytes, antimicrobial substances, hemolysins, and hemagglutinins (Bang and Lemma ; Unkles ; Jangoux ).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Establishing a Diagnostic Technique for Coelomocentesis in the Long‐Spined Sea Urchin Diadema antillarum

et al. 2018

View full text Add to dashboard Cite

Coelomic fluid aspiration has been utilized in echinoderms in research and clinical settings. Detailed procedural descriptions for coelomic fluid sampling in sea urchins (class Echinoidea) are lacking, and samples are prone to contamination. The objectives of this study were to (1) standardize a technique for coelomic fluid collection in long-spined sea urchin Diadema antillarum that optimizes the diagnostic quality of the sample utilizing diagnostic imaging, (2) identify coelomic fluid bacterial isolates (using Biolog GEN III MicroLog and 16s rDNA sequencing), and (3) compare positive cultures to animal weight, holding time prior to sampling, water temperature, and gross fluid appearance. Seventy Diadema antillarum from the Florida Keys collected in two groups (March and September 2015) were utilized. Positive cultures for bacterial contamination were identified in 5% and 44%, respectively, of animals in the sampling groups. Vibrio spp. was the predominant genus identified. Positive cultures were more frequent in the group with smaller-sized animals, increased holding times, and elevated water temperatures. Deviation from clear-pink gross coelomic fluid appearance did not reliably predict bacterial contamination. A standardized technique for coelomocentesis was defined. The use of the proposed coelomocentesis methodology may facilitate improved health evaluations of sea urchins and may be applicable to research, conservation efforts, and disease investigations.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations