Echinocytic Sensitivity and Deformability of Human Newborn Red Cells

Coulombel, Laure; Tchernia, G; C, Féo; Mohandas, Narla

doi:10.1159/000241611

Cited by 16 publications

(4 citation statements)

References 10 publications

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“…Those features that might affect their use include a predominance of Hb F with a higher oxygen affinity, a shortened iri vivo life-span, increased proportion of morphologic abnormalities, greater susceptibility to lipid peroxidation, altered membrane lipid fluidity, and more labile ATP levels (26)(27)(28)(29). Ultimately, clinical studies evaluating in vivo posttransfusion red cell survival will determine whether transfused autologous placental blood is as satisfactory as donor blood.…”

Section: Discussionmentioning

confidence: 99%

Collection and 28-Day Storage of Human Placental Blood

et al. 1994

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Placental blood was harvested using a novel collection system into packs containing citrate-phosphate-dextroseadenine and then stored for 28 d. Before and during storage, sterility, adequacy of anticoagulation, blood chemistry, and red cell viability were assessed weekly. The average volume of blood collected was 65 mL (range 30-110 mL) with a 0.42 hemocrit once diluted in anticoagulant. All blood culture specimens were sterile at collection and during storage. In the United States, more than 40 000 VLBW infants are born each year, many of whom are critically ill and require care in neonatal intensive care facilities. The majority of these infants will require one or more homologous blood transfusions during the first month of life. These transfusions are given to replace losses due to diagnostic sampling and to treat the physiologic decline in H b concentration designated the anemia of prematurity (1-3). Studies have shown that VLBW infants will receive approximately 50 mL of blood per kg from as many as eight to 10 adult donors during the first month of life (1, 3, 4). However, recent data suggest that changes in transfusion practices for neonates may reduce donor exposure (5, 6). Although red cell transfusion is a valuable part of care, the use of adult donor blood poses risks for these infants including the transmission of viral agents such as non-A and non-B hepatitis, cytomegalovirus, and human immunodeficiency virus (1,7,8). Furthermore, graft versus host disease has been reported after homologous transfusions in neonates (8-1 1). were >90 s, presumably because of the decline in labile clotting factors after 24 h of storage. Hematocrit, red cell ATP, and red cell shape were maintained during storage. As expected, red cell 2,3-diphosphoglycerate declined and potassium levels rose significantly but were not different than levels reported for adult cells similarly preserved. Based on our results, it appears that placental blood represents a potential source of autologous transfusion for the sick neonate over the first month of life. (Pediatr Res 36: W94, 1994)

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Section: Discussionmentioning

confidence: 99%

Collection and 28-Day Storage of Human Placental Blood

et al. 1994

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“…This should accelerate the shape transfornations in neonates if the incorporation rate is the same in neonates and adults. It has also been shown that neonatal erythrocytes are sensitive to slightly lower concentrations of LPC after 5 min of incubation (Coulombel et al 1982), while the proportion of cupped cells with CPZ after 15 min of incubation is the same in the cells of neonates as in the case of adults, and that found with high CPZ concentrations is even higher (Matovcik et al 1985).…”

Section: Number Of Cells Counted Varied From 53 To 180 the Times Reqmentioning

confidence: 99%

The kinetics of drug–membrane interactions in human erythrocytes from neonates

Eskelinen¹

1988

Can. J. Physiol. Pharmacol.

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The kinetics of drug-membrane interactions of erythrocytes from neonates were compared with those from adults by monitoring the time course of the shape transformations and vesicle release caused by drugs, using a light microscope--video recording technique. Both crenation caused by lysophosphatidylcholine (LPC) and cupping caused by chlorpromazine (CPZ) took place more slowly in the neonatal cells than in the cells from adults. The equilibrium concentrations of LPC and CPZ in erythrocytes did not differ significantly between the neonates and adults, however. The slower responses of the neonatal erythrocytes can be explained by the presence of negatively charged phosphatidylethanolamine and phosphatidylserine in the outer layer of the erythrocyte membrane, which may reduce the rate of incorporation of amphipathic LPC and attract cationic CPZ to remain in the outer membrane layer, lowering the rate of inward bending of the membrane normally caused by CPZ.

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“…Studies on the deformability from fetal erythrocytes have been controversial [1,2,4,8,11,16,18], because it is considered that obtained data depend on the difference of measurement methods. It should be stressed that we hypothesize that deformability of fetal erythrocytes is increased for the reason as mentioned above.…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte deformability of nonpregnant, pregnant, and fetal blood

Eguchi

Sawai²,

Mizutani³

1995

Jpme

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Erythrocyte deformability is an important determinant of microcirculation, of oxygen transport and release to the tissue. In an attempt to clarify the rheological peculiarity during pregnancy, erythrocyte deformability was measured in 10 nonpregnant controls and 10 pairs of mothers and newborns. When the hematocrit of the erythrocyte suspension in dextran solution was adjusted to 35%, the deformability of fetal erythrocytes was significantly higher than maternal blood (P < 0.05), and it was almost similar to that of nonpregnant controls. Erythrocyte deformability was dependent on the hematocrit, and there was an optimal hematocrit value at which the deformability was maximal. The hematocrit, where the deformability was maximal, was a lower value (32-35%) in maternal blood, conversely, a higher value (47-50%) in cord blood than that (40-43%) in the nonpregnant. Despite decreased deformability of maternal erythrocytes, it may be compensated by reducing the hematocrit (hemodilution), preserving effective peripheral circulation including uteroplacental perfusion. On the other hand, the deformability of fetal erythrocytes may be higher in vivo at the hematocrit of 47-50% to supply oxygen to the fetal tissue even in a low oxygen condition.

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Echinocytic Sensitivity and Deformability of Human Newborn Red Cells

Cited by 16 publications

References 10 publications

Collection and 28-Day Storage of Human Placental Blood

Collection and 28-Day Storage of Human Placental Blood

The kinetics of drug–membrane interactions in human erythrocytes from neonates

Erythrocyte deformability of nonpregnant, pregnant, and fetal blood

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