Echinococcus granulosus protoscoleces promotes proliferation and invasion of hepatocellular carcinoma cells

Yasen, Aimaiti; Wang, Maolin; Ran, Bo; Lv, Guodong; Aji, Tuerganaili; Xiao, Hui; Shao, Yingmei; Wen, Hao

doi:10.1007/s10616-020-00437-0

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…Contrary to our result, a recent study has shown that the E. granulosus PSCs did not induce significant differentiation in the morphology of HCC cells, but their proliferation and pathological properties were more altered after co-culturing with different quantities of PSCs in-vitro. Although they concluded that the mechanisms involved in the promoting effects of E. granulosus PSCs on proliferation, migration and invasion capacities of HCC cells, further investigation on the molecular mechanisms of processes is required [ 30 ]. The inconsistent results between studies may have been due to the fact that the whole HCF was used in our study and they only used PSCs.…”

Section: Discussionmentioning

confidence: 99%

Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells

Baysal,

Örsten,

Cengiz

et al. 2024

Acta Parasit.

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Cystic Echinococcosis (CE) is a zoonotic infection caused by the larval form of Echinococcus granulosus in humans. Emerging evidence suggests an intriguing inverse association between E. granulosus infection and the occurrence of cancer. This study aimed to investigate the influence of diverse host-derived hydatid cyst fluids (HCF) with distinct genotypes on human liver hepatocytes (HC) and hepatocellular carcinoma cells (HepG2). Specifically, we examined their effects on cell proliferation, apoptosis sensitivity (BAX/BCL-2), apoptosis-related p53 expression, and the expression of cancer-related microRNA (hsa-miR-181b-3p). Cell proliferation assays, real-time PCR, and ELISA studies were conducted to evaluate potential anti-cancer properties. The findings revealed that animal-origin HCF (G1(A)) induced direct cell death by augmenting the susceptibility of HepG2 cells to apoptosis. Treatment with both G1(A) and G1(H) HCF sensitized HepG2 and HC cell lines to apoptosis by modulating the BAX/BCL-2 ratio, accompanied by upregulation of the p53 gene. Additionally, G1(A) HCF and human-derived HCFs (G1(H), G7(H)) reduced the expression of miR-181b-3p in HepG2 cells. Consequently, this study demonstrates the potential anti-cancer effect of HCF in HepG2 cells and provides the first comparative assessment of HCFs from human and animal sources with diverse genotypes, offering novel insights into this field.

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Section: Discussionmentioning

confidence: 99%

Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells

Baysal,

Örsten,

Cengiz

et al. 2024

Acta Parasit.

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“…The procancerogenic effect of an echinococcus infestation related to the modulation of the host immune response has been pointed out in a few stuides [ 6 , 12 , 13 ]. In recent research, in vitro and ex vitro studies have shown that Echinoccocus granulosus protoscoleces have a promoting effect on the growth, migration and invasion capacities of HCC cells [ 22 ]. If HCC develops adjacent to CE, it might be speculated that a chronic inflammatory reaction in the surrounding parenchyma induces HCC development [ 11 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatocellular Carcinoma Presenting Simultaneously with Echinococcal Cyst Mimicking a Single Liver Lesion in a Non-Cirrhotic Patient: A Case Report and Review of the Literature

et al. 2022

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A coexistance of liver cystic echinococcosis (CE) and hepatocellular carcinoma (HCC) is very rare. HCC is the leading cause of cancer-related mortality worldwide, while CE is a globally endemic zoonosis caused by the cestode tapeworm Echinococcus granulosus. The association between these two diseases is still not well-defined. A preoperative diagnosis may be challenging, especially if HCC and CE present as a single lesion and if atypical imaging features are present. Herein, we present a case of the patient that was initially diagnosed as an extensive necrotic tumor in the left liver lobe and highly suspicious of being HCC associated with peritumoral hematoma. Left hemihepatectomy was performed, and the histopathological findings showed the collision of two lesions: a hydatid cyst and HCC.

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“…Daha sonra aynı çalışma grubu, kist hidatik sıvısının 78 kDa fraksiyonunun, 4T1 meme tümörlerine karşı bağışıklama için en etkili fraksiyon olduğunu belirlemişlerdir (7) . Öte yandan, yakın tarihli bir çalışma, E. granulosus protoskolekslerinin hepatoselüler kanser hücrelerinin çoğalmasını ve invazyonunu destekleyebileceğini bildirilmiştir (29) . Birçok rapor, kanserler ve parazitler arasında kanser oluşumuyla yakından ilişkili bazı ortak özellikler ve antijenler olduğunu göstermiştir (10,15,18) .…”

Section: Discussionunclassified

Echinococcus Granulosus Sensu Stricto Kaynakli Hi̇dati̇k Ki̇st Sivisi Uygulamasinin Caco-2 Hücre Hattinda Epi̇telyal-Mezenki̇mal Geçi̇ş Ve Apoptoz Üzeri̇ne Etki̇si̇

Baysal

Örsten

2022

ANKEM Dergisi

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Kistik ekinokokkoz (KE), Echinococcus granulosus sensu lato'nun larva formunun neden olduğu zoonotik bir enfeksiyondur. Yapılan çalışmalar E. granulosus enfeksiyonu ile kanser arasında doğrudan ve/veya dolaylı bir ilişki olduğu öne sürmüş; ancak, elde edilen sonuçlar farklı hücre kültürü ve/veya hayvan modellerinde hem anti-kanserojen hem de kanserojen etkisi olabileceğini göstermiştir. İnsan kolorektal adenokarsinom (Caco-2) hücrelerine etkisi daha önce değerlendirilmemiştir. Bu çalışmanın amacı, hidatik kist sıvısı uygulamasının bazı apoptotik genlerin (BCL-2, p53 ve BAX) ve epitelyal-mezenkimal geçiş (EMT) genlerinin (e-kaderin ve vimentin) ekspresyonu ve hücre proliferasyonu üzerine etkisini değerlendirerek Caco-2 hücre hattında olası anti-kanserojen veya kanserojen etkisini moleküler düzeyde aydınlatmaktır. Hidatik kist sıvısı uygulamasının sonrasında hücre proliferasyonu, apoptotik genler ve EMT gen ekspresyonu üzerindeki etkisini değerlendirmek için hücre proliferasyon analizi (XTT ile) ve gerçek zamanlı polimeraz zincir reaksiyonu (RT-PCR) yapılmıştır. Uygulama sonrasında uygulama dozu ile orantılı olarak hücre proliferasyonunda istatistiksel olarak anlamlı bir artış tespit edilmiştir. BAX ve p53 gen ifadelerinde doza bağlı azalma ve BCL-2 gen ifadesinde artış tespit edilmiştir. Bunun yanı sıra hücre kültüründe EMT gözlenmiş ve e-kaderin (CDH1) ve vimentin ekspresyonları ile moleküler düzeyde doğrulanmıştır. Bu çalışma ile hidatik kist sıvısının, Caco-2 hücre hattına uygulanması hücre proliferasyonunu doğrudan arttırdığı ve Caco-2 hücre hattının apoptoza karşı çok daha dirençli ve metastatik hale gelmesine neden olduğu tespit edilmiştir. Bu çalışma, ilk kez Caco-2 hücre hattından apoptotik yolakta hidatik kist sıvının olası mekanizmasına ışık tutmaktadır.

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Echinococcus granulosus protoscoleces promotes proliferation and invasion of hepatocellular carcinoma cells

Cited by 8 publications

References 32 publications

Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells

Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells

Hepatocellular Carcinoma Presenting Simultaneously with Echinococcal Cyst Mimicking a Single Liver Lesion in a Non-Cirrhotic Patient: A Case Report and Review of the Literature

Echinococcus Granulosus Sensu Stricto Kaynakli Hi̇dati̇k Ki̇st Sivisi Uygulamasinin Caco-2 Hücre Hattinda Epi̇telyal-Mezenki̇mal Geçi̇ş Ve Apoptoz Üzeri̇ne Etki̇si̇

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