Echinococcus granulosus: Insights into the protoscolex F-actin cytoskeleton

La-Rocca, Silvana; Farías, Joaquina; Chalar, Cora; Kun, Alejandra; Fernández, Verónica

doi:10.1016/j.actatropica.2019.105122

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…Studies have shown the presence of seven actin isoforms in T. solium [ 40 ] and six in E. granulosus [ 41 ]. The protein has been identified mainly in the tegument and in the protenephridial system, specifically in the terminal part of flame cells ( Figure 2 ) [ 21 , 33 ].…”

Section: Cytoskeletal Proteins Identified In Tapewormsmentioning

confidence: 99%

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

et al. 2023

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Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of the tapeworm cytoskeleton is linked to the medical importance of these parasitic diseases in humans and animal stock. Moreover, its study could offer new possibilities for the development of more effective anti-parasitic drugs, as well as better strategies for their surveillance, prevention, and control. In the present review, we compile the results of recent experiments on the cytoskeleton of these parasites and analyze how these novel findings might trigger the development of new drugs or the redesign of those currently used in addition to supporting their use as biomarkers in cutting-edge diagnostic tests.

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Section: Cytoskeletal Proteins Identified In Tapewormsmentioning

confidence: 99%

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

et al. 2023

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“…La equinococosis quística (EQ), es causada por el estado larvario del Echinococcus granulosus (E. granulosus), cestodo parásito del phylum Platyhelminthes; cuya plasticidad del citoesqueleto le permite entrar y desarrollarse en el interior de sus huéspedes, completando así su ciclo de vida (La-Rocca et al, 2019). A partir de la capa germinatica interna de los quistes, se generan los protoescólices, que son las formas infectantes para el perro y otros cánidos que constituyen los huéspedes definitivos de la enfermedad.…”

Section: Introductionunclassified

“…Los protoescólices se han estudiado mediante microscopía óptica y algunas tinciones como azul tripán, faloidina, etc, permitiendo visibilizar sus estructuras internas, tales como las ventosas, que exhiben una importante musculatura compuesta por un conjunto de fibras radiales, los corpúsculos calcáreos, la corona de ganchos y el rostelo con los filamentos de actina F que se encuentran dispuestos en forma bulbar con perforaciones que parecen ser lugares de unión de los ganchos (La-Rocca et al, 2019).…”

Section: Introductionunclassified

Estudio Morfocuantitativo de Protoescólices y otras Estructuras Fundamentales de Echinococcus granulosus en Hospederos Humanos

2022

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RESUMEN: La equinococosis quística (EQ) a pesar de ser una enfermedad endémica en diversos lugares del planeta, presenta pocos estudios morfológicos y cuantitativos de las estructuras fundamentales del Echinococcus granulosus en humanos, en especial de los protoescólices. El objetivo de este estudio fue analizar morfocuantitativamente protoescólices y otras estructuras fundamentales de E. granulosus obtenidos de hospederos humanos. Estudio de corte transversal. Se estudiaron 8 especímenes de EQ hepática humana, aplicando un muestreo no probabilístico por conveniencia. Se evaluó las capas quísticas, el líquido y la arenilla hidatídica. Las capas fueron fijadas en formaldehido tamponado a 10 % y procesadas para su inclusión en paraplast. Se realizaron cortes de 5 µm de grosor y fueron teñidas con H-E para su análisis con microscopía óptica. El líquido y arenilla fueron centrifugados y al sedimento obtenido se le realizó análisis directo para determinar las medidas morfométricas de los protoescólices y de los ganchos grandes y pequeños. Se utilizó estadística descriptiva. De los 8 quistes estudiados, 6 eran quistes univesicular, uno multivesicular y un quiste abscedado, cuyas capas laminada y germinativa se encontraban bien definidas. Las vesículas prolígeras presentaban forma redondeada con protoescólices en su interior. Los protoescólices invaginados presentaron un largo y ancho promedio de 140,8 ± 34,3 µm y 106,2 ± 29,5 µm, respectivamente; y los desarrollados un largo de 237,2 ± 53,0 µm y ancho de 128,7 ± 32,0 µm. Los ganchos rostelares presentaron contornos suaves distribuidos en dos filas regulares. El promedio del largo total de los ganchos grandes y pequeños fue 20,1 ± 2,7 µm; el promedio del ancho total fue 7,4 ± 1,2 µm. En conclusión, las características morfocuantitativas de los ganchos de E. granulosus en humanos, son distintos a otras especies hospederas intermediarias y de otros Echinococcus spp. Es posible que el abandono del estado de resistencia de los protoescólices invaginado hasta su desarrollo genere implicancias epidemiológicas de interés.

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“…Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the Echinococcus granulosus, is an animal and human health problem of increasing economic and public health importance [1]. CE in animals is asymptomatic and its diagnosis is made at necropsy [2,3], which depends on the analysis of morphological features using light or electron microscopy [4,5]. The serological diagnosis of CE improves the quality of the management and treatment of the disease.…”

Section: Introductionmentioning

confidence: 99%

A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis

et al. 2020

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Background: Cystic echinococcosis (CE), a zoonotic disease that affects animal and human health, is of increasing economic importance due to high morbidity rates and high economic losses in the livestock industry. Aim: The present study was conducted to purify the antigen from hydatid cyst fluid (HCF) with high diagnostic efficacy of camel hydatidosis using indirect enzyme-linked immunosorbent assay (ELISA). Materials and Methods: The HCF antigen was purified using Sephacryl S-300 column chromatography. Characterization of fractions was performed using reducing and non-reducing sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Further, antibodies against Echinococcus granulosus cysts in camel serum were detected using indirect ELISA. Results: The purification process resulted in three fractions of antigens: FI, FII, and FIII. Indirect ELISA showed that higher diagnostic efficacy was observed in FI than in FII and FIII. Indirect ELISA, in which FI was utilized, showed 88% sensitivity and 91.7% specificity. Non-reducing SDS-PAGE showed that FI had two bands of molecular weights 120 and 60 kDa. Western blot analysis of FI demonstrated that 60, 38, and 22 kDa were antigenic bands when reacted with naturally infected camel sera with E. granulosus cysts. Using indirect ELISA, F1 recorded an infection percentage of 81.7% in randomly collected camel serum samples. Conclusion: FI is a promising antigen for accurate diagnosis of camel CE using indirect ELISA.

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Echinococcus granulosus: Insights into the protoscolex F-actin cytoskeleton

Cited by 11 publications

References 38 publications

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

Estudio Morfocuantitativo de Protoescólices y otras Estructuras Fundamentales de Echinococcus granulosus en Hospederos Humanos

A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis

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