Echinococcus granulosus: Distribution of hydatid fluid antigens in tissues of the larval stage

Ultrastructural localization of major hydatid fluid antigens in brood capsules and protoscoleces of Echinococcus granulosus of human origin

“…In a previous study (Sanchez et al 1991), the avidinbiotin-complex immunoperoxidase technique was used to localize Ag 5 and Ag B in the cyst membranes and protoscoleces of E. granulosus of human origin. Monospecific antisera against major parasite antigens, obtained by rabbit immunization with protein fractions previously separated by polyacrylamide gel electrophoresis (Madico et al 1988) were used. In agreement with others studies (Yarzabal et al 1976, Rickard et al 1977, Davies et al 1978 of parasite material of ovine or equine origin, Ag 5 and AgB were associated with the germinal membrane and with the parenchyma of protoscoleces. However, because of the specificity of the antisera used or different antigenic expression within E. granulosus strains, we did not observe Ag B in the laminar membrane nor in the protoscolex tegument.…”

“…The parenchymal cells and the tegumental region have been implicated in the production of Ag 5 (Yarzabal et al 1976, Davies et al 1978 and Ag B (Yarzabal et at. 1977, Davies t t al.…”

Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin

“…Moreover, the amplification of the antigen-antibody reaction with the ABC method permits the use of low concentration of antibodies and minimizes the background staining, and this increases the specificity. In the present study, the ABC method was used with monospecific antisera, to determine the localization of Ag 5 (Davies et al 1978), and the possible participation of the excretory system in the transport and release of Ag 5 has been denoted (Yarzabal et al 1976, Davies et al 1978. However, antigen liberation by degeneration of the protoscoleces into the cyst lumen has also been suggested (Davies er al.…”

“…The two antigens have been localized in equine and ovine E. granulosus protoscoleces and cyst membranes by means of immunofluorescence (Yarzabal et al 1976; and immunoperoxidase techniques (Rickard et al 1977), and a peroxidase-antiperoxidase technique (Davies et al 1978) has been used to study their ultrastructural localization in Correspondence: Fernando Sanchez brood capsules and protoscoleces. Ag 5 was shown to be associated with the parenchymal cells of the protoscoleces, the brood capsule wall and the germinal membrane (Yarzabal et al 1976, Rickard et al 1977, Davies et al 1978, it is also present in the superficial layers of the laminated membrane (Rickard et al 1977). Ag B has been located on a variety of tissues, including the tegument of the protoscoleces (Yarzabal et al 1977, Rickard et al 1977, Davies et al 1978, the brood capsule wall (Rickard et al 1977, Davies ef al.…”

Cinética de la repuesta serólógica del ratón trás la infección primaria experimental con embrióforos de Echinococcus granulosus

“…Trabajos posteriores (8,11) demuestran que el antígeno responsable del arco 5° (antígeno 5) es compartido por la membrana germinativa y el tegumento y parén-quima de los protoescolex, así como por E. multilocularis otros cestodes vecinos como Taenia hydatígena (8).…”