Echinococcosis/Hydatidosis

Soriano, Antoni; Gmez, Frederic

doi:10.5772/25971

Cited by 2 publications

(6 citation statements)

References 22 publications

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“…Modern studies aimed to combat human and animal tapeworms, including Echinococcus tapeworm pathogen. However, they significantly reduced the level of invasion in most countries, such as Russia and the Commonwealth of Independent States, but it was less effective in Kazakhstan and Central Asian republics 6,7 . However, in most of these countries, infestation caused by Echinococcus tapeworms is hyperendemic to humans and animals.…”

Section: Introductionmentioning

confidence: 98%

“…Currently, the prevention of parasite infection is carried out by immunizing sensitive animals with oncospheres antigens (vaccines) 6,7,8,9 . Scientists use invasive tapeworm eggs to produce protective biologics.…”

Section: Introductionmentioning

confidence: 99%

“…Reliable methods of determining the quality of biological material are required to assess the eggs or the tapeworm oncospheres. Previously, the viability and invasive capability of taeniid eggs were determined by appearance and staining with paints and setting a biological test 2,6,10 . The morphological structure of taeniid eggs does not provide reliable information about viable, dead, and dying embryos.…”

Section: Introductionmentioning

confidence: 99%

“…A biological test on laboratory animals gives the result only after a few months but does not show the percentage of helminth quality eggs in the culture. Paint solutions (methylene blue, gencian violet, neutral red) color the eggs of dead tapeworms well and do not stain viable eggs and oncospheres 6,7,10 . In this method, the percentage of quality material is not clear.…”

Section: Introductionmentioning

confidence: 99%

“…In this method, the percentage of quality material is not clear. The most promising method of determining the viability and activation of tapeworm oncosphere is the cultivation method in natural or artificial digestive juices of hosts 6,7,11 . Activation of tapeworm eggs is determined by hatching the embryo from the eggshell and observing the physiological activity of oncospheres under the influence of temperature and/or enzyme factors.…”

Section: Introductionmentioning

confidence: 99%

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Biological Properties of Oncospheres of Echinococcus spp.

Andreyanov

2022

J. Vet. Physiol. Pathol.

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Introduction: Taeniasis is known as a global human and animal parasite. Infestation by Echinococcus has significantly decreased in some countries as a result of modern research aiming to combat this type of tapeworm. To obtain protective biologics, biomass of inactivated material is required. The present study aimed to obtain the maximum number of oncospheres of the genus Echinococcus for the preparation of vaccines. Materials and methods: Echinococcus multilocularis (E. multilocularis) eggs were placed in artificial intestinal juice. A beaker with juice and eggs was placed on a heated magnetic stirrer. To test the biological properties of the culture, Echinococcus eggs were used for infection of the laboratory mice. For infection, four groups of mice were used. Echinococcus eggs were activated in artificial intestinal juice on a magnetic stirrer in the first group. In the second group, cestode eggs were activated under thermostat conditions (38°C) in artificial gastric and, then, in intestinal juice. The third group was a positive control. The last group was a negative control. The selection of E. multilocularis metastases in laboratory mice was performed for 13 months. Cestodes parasitic cysts were evaluated in each infected mouse at the end of the experiment. The physiological status of E. multilocularis cysts in mice was assessed by a helminthological autopsy. The viability and activity of E. multilocularis protoscoleces were evaluated by motor activity. Mobility was recorded when heating the samples at 37°C for 10-15 minutes. Results: In the first group, second, and positive control groups, 70%, 44.4%, and 33.3% of the mice were sensitive to the alveococcus causative agent, respectively. The larval cysts of the cestodes were identified in the liver and lungs of the mice in the first experimental group. In the second and third groups, the larval in the form of the alveococcus was identified only in the liver. Conclusion: This method allows the investigation of the main biological indicators of cestode egg culture (viability, invasive activity). The novelty of the method is using only artificial intestinal juice without artificial gastric juice. The method can increase yield of activated oncospheres in a short time.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological Properties of Oncospheres of Echinococcus spp.

Andreyanov

2022

J. Vet. Physiol. Pathol.

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Prevalence, transmission dynamics of hydatidosis in slaughtered animals in Dodoma municipality, Tanzania

Mosha

Ezekiel

Mawaka³

et al. 2022

int.jour.sci.res.mana.

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BackgroundCystic Echinococcosis (CE) has been reported to exist both in humans and animals affecting people with their livestock. The study aimed to examine the prevalence of CE in slaughtered animals; practices facilitating transmission and perceived risk of human infection in Dodoma municipality, Tanzania.MethodsA quantitative cross-sectional study was conducted. Prevalence of CE was determined by physical examination of organs from slaughtered animals. Collected cysts were preserved in formalin, and then transported to Parasitology laboratory for establishment of fertility. A questionnaire was used to collect data. Data analysis was done by Statistical Package of Social Sciences software. Frequency tables on the prevalence of hydatidosis fertility rate of hydatid cysts, demographic characteristics, awareness, knowledge, risk perception and practices were tabulated. Chi square test was used to test relationship between variables related to awareness and knowledge on hydatidosis. Results Inspected animals for CE in different organs were 1,485, among them700 were cattle, 430 goats and 355 sheep. Overall prevalence of CE in all animals was 4.7%; and 7.6%, 2.1% and 2.3% for cattle, goats and sheep, respectively. Fertility rate was; 50.9% in cattle, 33.3% in goats, and 50% in sheep. The questionnaire involved 361 respondents. Majority 91.7% had low knowledge, 8.3% moderate on CE. Backyard-slaughtering, free-range dogs and livestock keeping, feeding offal to dogs and close relationship between dogs, humans and livestock kept the community at risk of acquiring CE. Conclusion CE was high among ruminant animals in Dodoma. Majority of participants had limited awareness on modes of transmission and practices that put them at risk. Close proximity to dogs indicates the potential risk for human infection. Collaboration between medical and veterinary sectors is recommended to raise community awareness and address the problem.

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Echinococcosis/Hydatidosis

Cited by 2 publications

References 22 publications

Biological Properties of Oncospheres of Echinococcus spp.

Biological Properties of Oncospheres of Echinococcus spp.

Prevalence, transmission dynamics of hydatidosis in slaughtered animals in Dodoma municipality, Tanzania

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