Echinacoside Suppresses the Progression of Breast Cancer by Downregulating the Expression of miR-4306 and miR-4508

Abstract

The main treatment of breast cancer includes surgical resection, radiotherapy, chemotherapy, endocrine therapy, and molecular targeted therapy, but the outcomes remain unsatisfactory. Previous studies demonstrated that echinacoside, microRNA (miRNA/miR)-4306 and miR-4508 were associated with lymph node metastasis, chemoresistance and self-renewal capability in breast cancer, but in-depth studies on the underlying mechanism of their anticancer effects have not been performed to date. In order to identify the role of miR-4306 and miR-4508, and the mechanism of the antitumor effect of echinacoside in breast cancer, the present study first examined the expression of miR-4306 and miR-4508 in breast cancer tissues to examine their possible role in the development of breast cancer, then evaluated the effect of echinacoside on the expression of miR-4306 and miR-4508 on the viability, apoptosis, cell cycle, migration, and invasion abilities of breast cancer cells to explore the anti-cancer effect of echinacoside and the involvement of miR-4306 and miR-4508. Finally, the breast cancer cells and mice bearing breast cancer xenografts were treated with echinacoside and inhibitors of miR-4508 or miR-4306 to confirm their role on the anticancer effect of echinacoside. The results showed that miR-4306 and miR-4508 were decreased in breast cancer tissues and cells. Echinacoside inhibited cell proliferation, invasion and migration, and promoted the apoptosis of breast cancer cells by downregulating the expression of miR-4306 and miR-4508. In conclusion, this is the first study to show the association between echinacoside and miRNAs in cancer. The present study elucidates an underlying molecular mechanism of the antitumor effect of echinacoside on breast cancer, and thus may contribute to preventive and therapeutic strategies for breast cancer.

Ekinakozit PC3 androjen bağımsız prostat kanseri hücrelerinde hücre proliferasyonunu azaltır ve hücre invazyonunu inhibe eder

“…Various molecular biological studies on the anti-cancer activity of echinacoside have been demonstrated both in vitro and in vivo in some studies. In these studies, echinacoside has been reported to reduce cell proliferation, cell invasion and migration-inhibited colony formation of various cancer cell lines [14,15,[20][21][22]27]. It was recently reported that echinacoside inhibits breast cancer cell growth under in vitro conditions via Wnt/ β-catenin signaling pathway.…”

“…Neuroprotective, hepatoprotective, immune modulator, antidiabetic and anticancer activities of echinacoside have been demonstrated in previous studies [15][16][17][18][19]. It has been reported that echinacoside exhibits anticancer activity through cellular mechanisms such as triggering apoptosis, cell cycle arrest, and inhibition of cell proliferation in various cancers such as breast [14] colon [15], pancreas [20], ovary [21] and liver [22]. There is no comprehensive molecular mechanistic study on echinacoside and prostate cancer in the literature.…”

“…Echinacoside is a natural small phenylethanoid glycoside molecule first isolated from Echinacea root. Echinacoside can also be extracted from Cistanche, which is widely used in Europe and is known to be good for colds and infections [13,14].…”

Echinacoside Inhibits Osteoclast Function by Down-Regulating PI3K/Akt/C-Fos to Alleviate Osteolysis Caused by Periprosthetic Joint Infection

“…Echinacoside (ECH), a natural phenylethanoid glycoside, is the main active ingredient presenting in Cistanche salsa, which has been reported to be extensively studied in neuroprotection (Lu et al, 2016), antitumor (Ye et al, 2019), anti-aging (Wu et al, 2019), and myocardial remodeling (Ni et al, 2021), antioxidant (Chuang et al, 2022), anti-apoptotic (Zhang et al, 2017), and anti-inflammatory (Zhou et al, 2020). Studies have demonstrated that ECH alleviates hypoxia-induced memory impairment (Zheng et al, 2019), inhibits the development of breast cancer (Bian et al, 2021), alleviates inflammatory bowel disease (Li et al, 2018), and improves heart failure by reversing myocardial remodeling (Ni et al, 2021). ECH has also been found to promote bone formation by enhancing osteoblast proliferation and differentiation as well as alleviating the reduction in bone mass caused by diabetes (Gong et al, 2019) or ovariectomy (Liu et al, 2019).…”

Exploration of the Effect and Potential Mechanism of Echinacoside Against Endometrial Cancer Based on Network Pharmacology and in vitro Experimental Verification

“…Echinacoside (ECH) is a primary natural bioactive ingredient derived from Cistanches Herba and Echinacea that has a variety of biological effects, such as neuroprotection, 13 myocardial protection, 14 liver protection, 15 immune regulation, 16 anti-tumor 17–20 and other pharmacological effects. In recent years, a line of studies has indicated that ECH has an anticancer effect on various tumors.…”