ECG Acquisition and Management System for Knowledge Discovery in Database: Data Modeling, Design, and Implementation

Cho, Yongho; Kim, Ho‐Sung; Jeon, Myoung-ju; Kang, Dong-Joo; Choi, Hyung-Sik; Lee, Jongmin; Kim, In-Young; Kim, Sun I.

doi:10.1007/3-540-36104-9_9

Cited by 1 publication

(1 citation statement)

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“…The ability to diagnose and monitor chronic health conditions like heart disease, diabetes, high blood pressure, cholesterol and cancer [1,2] is a key concern in medicine. Patients with these conditions are initially diagnosed with bloodwork and, once diagnosed, require regular blood monitoring [3][4][5] of the many health index markers found in blood. For reliability, convenience, and efficiency, implantable monitoring devices would be an obvious solution.…”

Section: Introductionmentioning

confidence: 99%

Miniaturized protein separation using a liquid chromatography column on a flexible substrate

Yang¹,

Chae²

2008

J. Micromech. Microeng.

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We report a prototype protein separator that successfully miniaturizes existing technology for potential use in biocompatible health monitoring implants. The prototype is a liquid chromatography (LC) column (LC mini-column) fabricated on an inexpensive, flexible, biocompatible polydimethylsiloxane (PDMS) enclosure. The LC mini-column separates a mixture of proteins using size exclusion chromatography (SEC) with polydivinylbenzene beads (5-20 μm in diameter with 10 nm pore size). The LC mini-column is smaller than any commercially available LC column by a factor of ∼11 000 and successfully separates denatured and native protein mixtures at ∼71 psi of the applied fluidic pressure. Separated proteins are analyzed using NuPAGE-gel electrophoresis, high-performance liquid chromatography (HPLC) and an automated electrophoresis system. Quantitative HPLC results demonstrate successful separation based on intensity change: within 12 min, the intensity between large and small protein peaks changed by a factor of ∼20. In further evaluation using the automated electrophoresis system, the plate height of the LC mini-column is between 36 μm and 100 μm. The prototype LC mini-column shows the potential for real-time health monitoring in applications that require inexpensive, flexible implant technology that can function effectively under non-laboratory conditions.

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