Ecdysteroid-inducible genes in the programmed cell death during insect metamorphosis

Tsuzuki, Seiji; Iwami, Masafumi; Sakurai, Shunsuke

doi:10.1016/s0965-1748(00)00124-7

Cited by 70 publications

(60 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In D. melanogaster, pulses of 20E throughout fly development have proved to regulate cell proliferation, differentiation, and programmed cell death in a highly controlled manner. During metamorphosis, for example, ecdysone is a primary regulator of apoptosis in larval tissues such as salivary glands, midgut and neural tissues which are destroyed or remodelled into an "adult" form (Mottier et al, 2004;Tsuzuki et al, 2001). The activation and execution of ecdysis (i.e.…”

Section: Ecdysteroids: a Role As Sex Hormones In Insects?mentioning

confidence: 99%

Sex Steroids in Insects and the Role of the Endosymbiont Wolbachia:A New Perspective

Negri¹,

Pellecchia²

2012

Sex Hormones

View full text Add to dashboard Cite

Section: Ecdysteroids: a Role As Sex Hormones In Insects?mentioning

confidence: 99%

Sex Steroids in Insects and the Role of the Endosymbiont Wolbachia:A New Perspective

Negri¹,

Pellecchia²

2012

Sex Hormones

View full text Add to dashboard Cite

“…Total RNA (1 μg) was extracted from brains according to the acid guanidium thiocyanate phenol-chloroform method (Chomczynski and Sacchi, 1987) with minor modifications (Tsuzuki et al, 2001), treated with RNase-free DNase I (Promega, Madison, WI, USA), and used as RNA sample. 20E-injected and Ringer-injected RNA samples were both independently labeled with Cyanine 3-CTP (Cy3) and Cyanine 5-CTP (Cy5).…”

Section: Microarray Analysismentioning

confidence: 99%

Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Interestingly, it has recently been shown that recombinant Sendai viruses expressing a short transcript that binds TIAR are non-cytopathic (23). Apoptosis triggered by 20-hydroxyecdysone during pupal metamorphosis of the silkworm Bombyx mori is accompanied by an increase in TIAR mRNA levels (43). Ischemia-induced apoptosis also leads to an increase in TIAR mRNA levels (44,45).…”

Section: Tia-1 or Tiar Requirement For Cell Viabilitymentioning

confidence: 99%

TIA-1 or TIAR Is Required for DT40 Cell Viability

Guiner

Gesnel

Breathnach

2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

TIA-1 and TIAR are a pair of related RNA-binding proteins which have been implicated in apoptosis. We show that chicken DT40 cells with both tia-1 alleles and one tiar allele disrupted (tia-1(؊/؊)tiar(؊/؉) cells) are viable. However, their growth and survival in medium containing low serum levels is significantly reduced compared with DT40 cells. The remaining intact tiar allele in tia-1(؊/؊)tiar(؊/؉) cells can only be disrupted if TIA-1 expression is first restored to the cells by transfection of a TIA-1 expression vector. We conclude that DT40 cells require either TIA-1 or TIAR for viability. TIA-1 overexpression in tia-1(؊/؊)tiar(؊/؉) cells leads to a radical drop in TIAR levels, by inducing efficient splicing of two tiar alternative exons carrying in-frame stop codons. In wild-type DT40 cells, tiar transcripts including these exons can also be detected. These transcripts increase significantly in abundance in cycloheximide-treated cells, suggesting that splicing of the exons exposes mRNAs to nonsense-mediated mRNA decay. TIA-1 or TIAR depletion leads to a marked drop in splicing of the exons. The human tiar gene contains a corresponding pair of TIA-1-inducible alternative exons, and we show that there is very high sequence conservation between chickens and humans of the exon pair and parts of the flanking introns. The TIA-1/TIAR responsiveness of these alternative tiar exons is likely to be of physiological importance for controlling TIAR levels.TIA-1 (1 ,2) and the TIA-1-related protein TIAR (3) are a pair of similar, ubiquitous RNA-binding proteins with three RNA recognition motifs (RRMs).1 These proteins fill important functions in both the cytoplasm and the nucleus, as do a number of other multifunctional regulatory proteins (4). In the cytoplasm, TIA-1 and TIAR control translation of some specific mRNAs (5-9) and are active in the general translational arrest mechanism induced by stress (10 -15). TIA-1 and TIAR shuttle between cytoplasm and nucleus, but are predominantly nuclear in most cells studied. In the nucleus, they activate splicing of exons with weak 5Ј-splice sites followed by uridylate-rich stretches (16 -18). TIA-1 and TIAR appear to bind to these stretches (they both bind preferentially to uridylate-rich sequences in vitro, Ref. 19) and assist binding of U1 snRNP to the adjacent 5Ј-splice site. Possible target exons include an fgfr-2 alternative exon (16), a Drosophila msl-2 exon (20), a human fas exon (20), and some alternative human tia-1 and tiar exons containing premature stop codons (21). TIAR and possibly TIA-1 are also involved in replication of the RNA genomes of West Nile virus (22).Overexpression of TIA-1 or TIAR induces apoptosis, whether accomplished by exposure of permeabilized cells to recombinant proteins (1, 3), or by infecting cells with an engineered virus (23). During Fas-induced apoptosis, TIA-1 is phosphorylated (24), and TIAR is relocated to the cytoplasm (25). TIA-1 activates splicing of a fas pre-mRNA exon so as to favor production of a cell death receptor, at the exp...

show abstract

Ecdysteroid-inducible genes in the programmed cell death during insect metamorphosis

Cited by 70 publications

References 28 publications

Sex Steroids in Insects and the Role of the Endosymbiont Wolbachia:A New Perspective

Sex Steroids in Insects and the Role of the Endosymbiont Wolbachia:A New Perspective

Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis

TIA-1 or TIAR Is Required for DT40 Cell Viability

Contact Info

Product

Resources

About