Ecdysteroid biosynthesis in crayfish Y‐organs: Feedback regulation by circulating ecdysteroids

Dell, Susanne; Sedlmeier, Dietrich; Böcking, Detlef; Dauphin‐Villemant, Chantal

doi:10.1002/(sici)1520-6327(1999)41:3<148::aid-arch6>3.3.co;2-l

Cited by 10 publications

(7 citation statements)

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“…20E has been proposed to affect ecdysteroid biosynthesis in Yorgan (Dell et al, 1999), chitin metabolism in epidermis (Qian et al, 2014;Rocha et al, 2012), and neuropeptide synthesis in eyestalk (Mattson and Spaziani, 1986;Techa and Chung, 2015). Accordingly, PtSad, PtChi1, and PtMIH were selected as the candidate downstream genes controlled by 20E.…”

Section: Discussionmentioning

confidence: 99%

“…7A), suggesting the expression of PtSad may be negatively regulated by PtE75. This finding may work in synchrony with the negative feedback regulation of 20E on Y-organ ecdysteroid synthesis proposed by Dell et al (1999), and may also partially account for the sharply decrease in PtE75 expression in Y-organ during early-premolt when the expression of PtSad was rapidly increasing (unpublished data in our group). However, we noticed that the expression of PtE75 and PtSad in Y-organ were both induced by ESA (Fig.…”

Section: Discussionmentioning

confidence: 99%

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The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes

Xie

Zhou

Liu

et al. 2016

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes

Xie

Zhou

Liu

et al. 2016

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…Opposing results for EcR expression between these two works may be due to the difference in exposure length (96 h versus 30 h) or due to a difference in the effects exerted by fipronil and fipronil desulfinyl. It is possible that after an initial upregulation of EcR during the pre-molting period that expression then declines, due the sudden drop-off in ecdysteroids that occurs in the late pre-molt stage and due to the negative feedback loop that regulates ecdysis in decapods (Dell et al, 1999;Chang and Mykles, 2011). However, this would need to be confirmed experimentally by measuring EcR expression in exposed juveniles at numerous time intervals during periods of molting.…”

Section: Discussionmentioning

confidence: 99%

The effects of fipronil and the photodegradation product fipronil desulfinyl on growth and gene expression in juvenile blue crabs, Callinectes sapidus, at different salinities

et al. 2017

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Endocrine disrupting compounds (EDCs) are now widely established to be present in the environment at concentrations capable of affecting wild organisms. Although many studies have been conducted in fish, less is known about effects in invertebrates such as decapod crustaceans. Decapods are exposed to low concentrations of EDCs that may cause infertility, decreased growth, and developmental abnormalities. The objective herein was to evaluate effects of fipronil and its photodegradation product fipronil desulfinyl. Fipronil desulfinyl was detected in the eggs of the decapod Callinectes sapidus sampled off the coast of South Carolina. As such, to examine specific effects on C. sapidus exposed in early life, we exposed laboratory-reared juveniles to fipronil and fipronil desulfinyl for 96h at three nominal concentrations (0.01, 0.1, 0.5μg/l) and two different salinities (10, 30ppt). The size of individual crabs (weight, carapace width) and the expression of several genes critical to growth and reproduction were evaluated. Exposure to fipronil and fipronil desulfinyl resulted in significant size increases in all treatments compared to controls. Levels of expression for vitellogenin (Vtg), an egg yolk precursor, and the ecdysone receptor (EcR), which binds to ecdysteroids that control molting, were inversely correlated with increasing fipronil and fipronil desulfinyl concentrations. Effects on overall growth and on the expression of EcR and Vtg differ depending on the exposure salinity. The solubility of fipronil is demonstrated to decrease considerably at higher salinities. This suggests that fipronil and its photodegradation products may be more bioavailable to benthic organisms as salinity increases, as more chemical would partition to tissues. Our findings suggest that endocrine disruption is occurring through alterations to gene expression in C. sapidus populations exposed to environmental levels of fipronil, and that effects may be dependent upon the salinity at which exposure occurs.

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“…At premoult or following XO-SG extirpation, reduced MIH secretion releases this inhibition, thus enabling ecdysteroid synthesis and the onset of premoult (reviewed in Chang et al, 1993). The subsequent decrease in the secretion of ecdysteroids prior to moult is directly affected by circulating 20E and indirectly by the stimulatory effect of 20E on X-organ secretion (reviewed in Dell et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Hepatopancreatic multi‐transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle

Yudkovski

Shechter

Chalifa‐Caspi

et al. 2007

Insect Molecular Biology

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Alterations of hepatopancreatic multi-transcript expression patterns, related to induced moult cycle, were identified in male Cherax quadricarinatus through cDNA microarray hybridizations of hepatopancreatic transcript populations. Moult was induced by X-organ sinus gland extirpation or by repeated injections of 20-hydroxyecdysone. Manipulated males were sacrificed at premoult or early postmoult, and a reference population was sacrificed at intermoult. Differentially expressed genes among the four combinations of two induction methods and two moult stages were identified. Biologically interesting clusters revealing concurrently changing transcript expressions across treatments were selected, characterized by a general shift of expression throughout premoult and early postmoult vs. intermoult, or by different premoult vs. postmoult expressions. A number of genes were differentially expressed in 20-hydroxyecdysone-injected crayfish vs. X-organ sinus gland extirpated males.

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Ecdysteroid biosynthesis in crayfish Y‐organs: Feedback regulation by circulating ecdysteroids

Cited by 10 publications

References 0 publications

The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes

The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes

The effects of fipronil and the photodegradation product fipronil desulfinyl on growth and gene expression in juvenile blue crabs, Callinectes sapidus, at different salinities

Hepatopancreatic multi‐transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle

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