Ecdysone‐responsive microRNA‐252‐5p controls the cell cycle by targeting Abi inDrosophila

Abstract

The steroid hormone ecdysone has a central role in the developmental transitions of insects through its control of responsive protein-coding and microRNA (miRNA) gene expression. However, the complete regulatory network controlling the expression of these genes remains to be elucidated. In this study, we performed cross-linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 (Ago1) protein, the core effector of the miRNA pathway, in Drosophila S2 cells. We found that regulatory interactions between miRNAs and their cognate targets were substantially altered by Ago1 in response to ecdysone signaling. Additionally, during the larva-to-adult metamorphosis, miR-252-5p was up-regulated via the canonical ecdysone-signaling pathway. Moreover, we provide evidence that miR-252-5p targets Abelson interacting protein ( Abi) to decrease the protein levels of cyclins A and B, controlling the cell cycle. Overall, our data suggest a potential role for the ecdysone/miR-252-5p/Abi regulatory axis partly in cell-cycle control during metamorphosis in Drosophila.-Lim, D.-H., Lee, S., Han, J. Y., Choi, M.-S., Hong, J.-S., Seong, Y., Kwon, Y.-S., Lee, Y. S. Ecdysone-responsive microR-252-5p controls the cell cycle by targeting Abi in Drosophila.

MicroRNA miR‐252 targets mbt to control the developmental growth of Drosophila

“…Results from previous Ago1 CLIP-seq have shown that mbt is a target of miR-252 (Lim et al, 2018). Similarly, we also observed that mbt knockdown in S2 cells reduced cell proliferation (Lim et al, 2018). Similarly, we also observed that mbt knockdown in S2 cells reduced cell proliferation (Lim et al, 2018).…”

“…Consistent with this finding, miR-252 expression is highly elevated during the pupal and adult stages of Drosophila development compared with the embryonic and larval stages (Lim et al, 2018). Loss of miR-252 increased the body weight of adult flies, whereas overexpression of miR-252 in the larval fat body reduced adult size and mass.…”

“…These regulatory networks involve various external and internal factors and ultimately determine the final body size and mass of animals. Furthermore, miR-252 acts as a negative growth regulator in S2 cells (Lim et al, 2018). Most organisms experience growth at the juvenile stages.…”

“…Results from previous Ago1 CLIP-seq have shown that mbt is a target of miR-252 (Lim et al, 2018). Results from previous Ago1 CLIP-seq have shown that mbt is a target of miR-252 (Lim et al, 2018).…”

“…RT-qPCR was then performed as described previously (Lim et al, 2018), and gene-specific primers are listed in Supporting Information Table S1. RT-qPCR was then performed as described previously (Lim et al, 2018), and gene-specific primers are listed in Supporting Information Table S1.…”