ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

Tian, Jichun; Rong, Liwei; Feng, Yang; Wang, Qi; Song, Qiulai; Zhang, Lixin; Ouyang, Ming

doi:10.1093/jxb/ery139

Cited by 28 publications

(32 citation statements)

References 48 publications

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“…With a different genetic approach based on a different mutant allele of the EMB2261 gene, we confirm the conclusion of Jiang et al (2018) , claiming that EMB2261/ECD1 is the editing specificity factor of rps14-2 in Arabidopsis chloroplasts and is essential for Arabidopsis development. Here, we discuss the implication of this finding from four aspects: (1) editing factor mutants as surrogate mutants of the corresponding genes; (2) PPR specificity factors as limiting factors in RNA editing; (3) editing specificity determinants beyond the PPR-RNA recognition code; and (4) co-evolutionary scenarios involving PPR RNA editing factors and their targets.…”

Section: Discussionsupporting

confidence: 73%

“…During the writing up of this work, an independent manuscript reported that ECD1 (synonymous with EMB2261) is required for editing of the rps14-2 site in Arabidopsis and is required for early chloroplast development ( Jiang et al, 2018 ). We confirm this conclusion with a different genetic approach and a different mutant allele of the EMB2261 gene, and expand these findings by considering the specificity of the EMB2261/ rps14-2 interaction and the evolutionary history of the pair.…”

Section: Introductionmentioning

confidence: 99%

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Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development

et al. 2018

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RNA editing in plastids is known to be required for embryogenesis, but no single editing event had been shown to be essential. We show that the emb2261-2 mutation is lethal through a failure to express an editing factor that specifically recognizes the rps14-2 site. EMB2261 was predicted to bind the cis-element upstream of the rps14-2 site and genetic complementation with promoters of different strength followed by RNA-seq analysis was conducted to test the correlation between rps14-2 editing and EMB2261 expression. Rps14-2 is the only editing event in Arabidopsis chloroplasts that correlates with EMB2261 expression. Sequence divergence between the cis-element and the EMB2261 protein sequence in plants where rps14-2 editing is not required adds support to the association between them. We conclude that EMB2261 is the specificity factor for rps14-2 editing. This editing event converts P51 in Rps14 to L51, which is conserved among species lacking RNA editing, implying the importance of the editing event to Rps14 function. Rps14 is an essential ribosomal subunit for plastid translation, which, in turn, is essential for Arabidopsis embryogenesis.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development

et al. 2018

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“…Contrary to the increasing information about the subfamily classification and organellar targeting of PPR proteins in plants, the cellular role and function of most PPR proteins are still so far from fully being apprehended (Lee et al, 2019). Among these PPR proteins, several mutants show pigment defective or lethal phenotypes, such as sel1 (Pyo et al, 2013), ecd1 (Jiang et al, 2018), ppr4, emb2654 (Lee et al, 2019), and sot5/emb2279 (Huang et al, 2018) in addition to pdm2 and pdm3 mutants (Du et al, 2017; which have been reported in our lab before. These works suggest that most functional PPR proteins located in the chloroplast mainly play a critical role in accumulation of chlorophyll and are essential for plant survival.…”

Section: Discussionmentioning

confidence: 74%

PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis thaliana

et al. 2020

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Extensive studies have been carried out on chloroplast gene expression and chloroplast development; however, the regulatory mechanism is still largely unknown. Here, we characterized Pigment-Defective Mutant4 (PDM4), a P-type PPR protein localized in chloroplast. The pdm4 mutant showed seedling-lethal and albino phenotype under heterotrophic growth conditions. Transmission electron microscopic analysis revealed that thylakoid structure was totally disrupted in pdm4 mutant and eventually led to the breakdown of chloroplasts. The levels of several chloroplast-and nuclear-encoded proteins are strongly reduced in pdm4 mutant. Besides, transcript profile analysis detected that, in pdm4 mutant, the expression of plastid-encoded RNA polymerasedependent genes was markedly affected, and deviant chloroplast rRNA processing was also observed. In addition, we found that PDM4 functions in the splicing of group II introns and may also be involved in the assembly of the 50S ribosomal particle. Our results demonstrate that PDM4 plays an important role in chloroplast gene expression and chloroplast development in Arabidopsis.

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“…Pentatricopeptide repeat (PPR) proteins form the largest family of proteins known to date that are directly involved in posttranscriptional modification in higher plants. Accumulating data indicate that PPR family members serve diverse functions in posttranscriptional modification, such as RNA editing (Ichinose & Sugita, 2018;Jiang et al, 2018;Xiao et al, 2018a,b), RNA splicing Huang et al, 2018;Tadini et al, 2018), RNA processing (Rackham et al, 2016;Stoll et al, 2017), RNA stability (Hammani et al, 2016;Zoschke et al, 2016;, RNA translation Legen et al, 2018), and RNA maturation (W. . The PPR gene family comprises 450 members in Arabidopsis and 477 members in rice (Saha et al, 2007;Barkan & Small, 2014).…”

Section: Introductionmentioning

confidence: 99%

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Cui

Wang

et al. 2018

New Phytologist

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Summary Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA‐binding protein, DUA1, cloned from the indica cultivar Dular, which exhibits a deficiency in chloroplast development at an early stage of development under low‐temperature conditions. DUA1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low‐temperature conditions. Our data suggest that DUA1 can bind to the plastid‐encoded rps8‐182 transcript and disruption of DUA1 activity impairs editing. The RNA editing cofactor WSP1, a partner of DUA1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures. DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low‐temperature conditions. Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.

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ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

Abstract: The pentatricopeptide repeat protein ECD1 functions as an RNA-editing trans-factor in plastids of cotyledons and is necessary for early chloroplast development in Arabidopsis seedlings.

Cited by 28 publications

References 48 publications

Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development

Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development

PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis thaliana

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

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