ECA3, a Golgi-Localized P2A-Type ATPase, Plays a Crucial Role in Manganese Nutrition in Arabidopsis

Mills, Rebecca F.; Doherty, Melissa Louise; López‐Marqués, Rosa L.; Weimar, Thilo; Dupree, Paul; Palmgren, Michael G.; Pittman, Jon K.; Williams, Lorraine E.

doi:10.1104/pp.107.110817

Cited by 159 publications

(167 citation statements)

References 58 publications

(80 reference statements)

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“…As CPA at a concentration that induced [Ca 2+ ] cyt oscillations did not completely deplete the [Ca 2+ ] ER in this study, a CPA-insensitive Ca 2+ -ATPase in the ER might be related with the [Ca 2+ ] cyt oscillations. In addition, ECA3, another IIA-type Ca 2+ -ATPase from Arabidopsis, has been detected in the Golgi apparatus (Mills et al, 2008), and a CPA-sensitive Ca 2+ pump has been identified in both ER vesicles and the Golgi apparatus of Pisum sativum (Ordenes et al, 2002). Furthermore, vesicle accumulation independent of the ER was observed in the pollen tip of L. longiflorum (Parton et al, 2003;Bove et al, 2008).…”

Section: Dynamics In Arabidopsis Pollen Tubesmentioning

confidence: 84%

Fine-Tuning of the Cytoplasmic Ca2+ Concentration Is Essential for Pollen Tube Growth

Iwano

Entani²,

Shiba³

et al. 2009

Plant Physiology

167

147

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Pollen tube growth is crucial for the delivery of sperm cells to the ovule during flowering plant reproduction. Previous in vitro imaging of Lilium longiflorum and Nicotiana tabacum has shown that growing pollen tubes exhibit a tip-focused Ca2+ concentration ([Ca2+]) gradient and regular oscillations of the cytosolic [Ca2+] ([Ca2+]cyt) in the tip region. Whether this [Ca2+] gradient and/or [Ca2+]cyt oscillations are present as the tube grows through the stigma (in vivo condition), however, is still not clear. We monitored [Ca2+]cyt dynamics in pollen tubes under various conditions using Arabidopsis (Arabidopsis thaliana) and N. tabacum expressing yellow cameleon 3.60, a fluorescent calcium indicator with a large dynamic range. The tip-focused [Ca2+]cyt gradient was always observed in growing pollen tubes. Regular oscillations of the [Ca2+]cyt, however, were rarely identified in Arabidopsis or N. tabacum pollen tubes grown under the in vivo condition or in those placed in germination medium just after they had grown through a style (semi-in vivo condition). On the other hand, regular oscillations were observed in vitro in both growing and nongrowing pollen tubes, although the oscillation amplitude was 5-fold greater in the nongrowing pollen tubes compared with growing pollen tubes. These results suggested that a submicromolar [Ca2+]cyt in the tip region is essential for pollen tube growth, whereas a regular [Ca2+] oscillation is not. Next, we monitored [Ca2+] dynamics in the endoplasmic reticulum ([Ca2+]ER) in relation to Arabidopsis pollen tube growth using yellow cameleon 4.60, which has a lower affinity for Ca2+ compared with yellow cameleon 3.60. The [Ca2+]ER in pollen tubes grown under the semi-in vivo condition was between 100 and 500 μ m. In addition, cyclopiazonic acid, an inhibitor of ER-type Ca2+-ATPases, inhibited growth and decreased the [Ca2+]ER. Our observations suggest that the ER serves as one of the Ca2+ stores in the pollen tube and cyclopiazonic acid-sensitive Ca2+-ATPases in the ER are required for pollen tube growth.

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Section: Dynamics In Arabidopsis Pollen Tubesmentioning

confidence: 84%

Fine-Tuning of the Cytoplasmic Ca2+ Concentration Is Essential for Pollen Tube Growth

Iwano

Entani²,

Shiba³

et al. 2009

Plant Physiology

167

147

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“…The P-type ATPases AtECA1 (localized at the endoplasmic reticulum) and AtECA3 (expressed in the Golgi complex) participate in the influx of Mn (Li et al, 2008;Mills et al, 2008). The cation/H exchanger (CAX) transporters AtCAX2 and AtCAX4, which were originally identified as Ca transporters, also have the ability to transport Mn into the vacuole (Connorton et al, 2012;Korenkov et al, 2007).…”

Section: Manganese Uptake By Plantsmentioning

confidence: 99%

“…In the chloroplasts of both Arabidopsis and barley, HMA1 contributes to Zn detoxification by exporting Zn from the plastids to the cytoplasm Mikkelsen et al, 2012). It has been proposed that the Golgi-localized transporter AtECA3 may have a role in the mobilization of Zn from the cytoplasm to the Golgi complex (Barabasz et al, 2011;Mills et al, 2008).…”

Section: Figmentioning

confidence: 99%

Influence of Soil Chemistry and Plant Physiology in the Phytoremediation of Cu, Mn, and Zn

Pinto

Aguiar

Ferreira

2014

Critical Reviews in Plant Sciences

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“…EF hands in K + channel-interacting proteins have been shown to be essential for correct trafficking of mammalian K + channel proteins (Flowerdew and Burgoyne, 2009). A role for Ca 2+ in the recognition and vacuolar sorting of the ER-like Ca/Mn-ATPase ECA3 and Ca 2+ -dependent recognition of cargo proteins has also been inferred (Li et al, 2008, Mills et al, 2008. Patch clamp recordings show little effect of cytoplasmic Ca 2+ on the activity of either TPKa or TPKb, indicating that the putative EF domains in these proteins may not be functional.…”

Section: The Tpk C Terminus Affects Tpk Membrane Localizationmentioning

confidence: 99%

Rice Two-Pore K+ Channels Are Expressed in Different Types of Vacuoles

2011

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Potassium (K + ) is a major nutrient for plant growth and development. Vacuolar K + ion channels of the two-pore K + (TPK) family play an important role in maintaining K + homeostasis. Several TPK channels were previously shown to be expressed in the lytic vacuole (LV) tonoplast. Plants also contain smaller protein storage vacuoles (PSVs) that contain membrane transporters. However, the mechanisms that define how membrane proteins reach different vacuolar destinations are largely unknown. The Oryza sativa genome encodes two TPK isoforms (TPKa and TPKb) that have very similar sequences and are ubiquitously expressed. The electrophysiological properties of both TPKs were comparable, showing inward rectification and voltage independence. In spite of high levels of similarity in sequence and transport properties, the cellular localization of TPKa and TPKb channels was different, with TPKa localization predominantly at the large LV and TPKb primarily in smaller PSV-type compartments. Trafficking of TPKa was sensitive to brefeldin A, while that of TPKb was not. The use of TPKa:TPKb chimeras showed that C-terminal domains are crucial for the differential targeting of TPKa and TPKb. Site-directed mutagenesis of C-terminal residues that were different between TPKa and TPKb identified three amino acids that are important in determining ultimate vacuolar destination.

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ECA3, a Golgi-Localized P2A-Type ATPase, Plays a Crucial Role in Manganese Nutrition in Arabidopsis

Cited by 159 publications

References 58 publications

Fine-Tuning of the Cytoplasmic Ca2+ Concentration Is Essential for Pollen Tube Growth

Fine-Tuning of the Cytoplasmic Ca2+ Concentration Is Essential for Pollen Tube Growth

Influence of Soil Chemistry and Plant Physiology in the Phytoremediation of Cu, Mn, and Zn

Rice Two-Pore K+ Channels Are Expressed in Different Types of Vacuoles

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