EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis

Yang, Zidong; Qian, Shuiming; Rn, Scheid; Lü, Ling; Chen, X; R, Liu; Du, Xiaohong; X, Lv; Boersma,; Scalf, Mark; Lm, Smith; Jm, Denu; Du, J.; Zhong, Xuehua

doi:10.1038/s41588-018-0187-8

Cited by 102 publications

(91 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These histone marks are catalyzed and removed by specific enzyme complexes that are referred to as the writers and erasers, and are recognized by reader proteins (Liu et al 2010). For example, H3K27me3, which is a representative repressive marker that exhibits high deposition on many stress‐responsive genes, is catalyzed by the polycomb repressive complex 2 (PRC2) via the histone methyltransferases CURLY LEAF (CLF), SWINGER (SWN), and MEDEA (MEA) and their cofactors; meanwhile H3K27me3 is recognized by the PRC1 complex via the H3K27me3 reader proteins LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), EARLY BOLTING IN SHORT DAY (EBS), and SHORT LIFE (SHL) and their cofactors in Arabidopsis (Mozgova and Hennig 2015; Li et al 2018; Yang et al 2018b). As an active marker, H3K4me3 is also present on a large number of stress‐responsive genes.…”

Section: Profile Of Plant Epigenetic Mechanismsmentioning

confidence: 99%

Epigenetic regulation in plant abiotic stress responses

Chang

Jiang

et al. 2020

JIPB

289

198

View full text Add to dashboard Cite

In eukaryotic cells, gene expression is greatly influenced by the dynamic chromatin environment. Epigenetic mechanisms, including covalent modifications to DNA and histone tails and the accessibility of chromatin, create various chromatin states for stress-responsive gene expression that is important for adaptation to harsh environmental conditions. Recent studies have revealed that many epigenetic factors participate in abiotic stress responses, and various chromatin modifications are changed when plants are exposed to stressful environments. In this review, we summarize recent progress on the cross-talk between abiotic stress response pathways and epigenetic regulatory pathways in plants. Our review focuses on epigenetic regulation of plant responses to extreme temperatures, drought, salinity, the stress hormone abscisic acid, nutrient limitations and ultraviolet stress, and on epigenetic mechanisms of stress memory.

show abstract

Section: Profile Of Plant Epigenetic Mechanismsmentioning

confidence: 99%

Epigenetic regulation in plant abiotic stress responses

Chang

Jiang

et al. 2020

JIPB

289

198

View full text Add to dashboard Cite

show abstract

“…The extent of the early flowering phenotype in long-day photoperiods varies across greenhouses (Zemach et al, 2013;Kang et al, 2015). Therefore, we included additional genotypes to control for this effect (ebs1 flowers early and hda6 flowers late; Yu et al, 2011;López-González et al, 2014;Yang et al, 2018). All mutants display a dwarf stature, with corresponding mass reductions of the fifth rosette leaf (means ranged 12.2 to 19.4 mg) relative to Col-0 (mean of 33.5 mg; n = 8, P , 0.001; Fig.…”

Section: Hda9 Pwr and Hos15 Regulate Pleiotropic Development Througmentioning

confidence: 99%

“…Full-length HDA9 and HOS15 complementary DNA (cDNA) were in-frame cloned into plasmids containing N-terminal and C-terminal part of YFP, respectively (Yang et al, 2018). The constructs were transformed into agrobacteria.…”

Section: Bimolecular Fluorescence Complementationmentioning

confidence: 99%

HDA9-PWR-HOS15 Is a Core Histone Deacetylase Complex Regulating Transcription and Development

et al. 2019

Self Cite

View full text Add to dashboard Cite

Histone deacetylases remove acetyl groups from histone proteins and play important roles in many genomic processes. How histone deacetylases perform specialized molecular and biological functions in plants is poorly understood. Here, we identify HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15 (HOS15) as a core member of the Arabidopsis (Arabidopsis thaliana) HISTONE DEACETYLASE9-POWERDRESS (HDA9-PWR) complex. HOS15 immunoprecipitates with both HDA9 and PWR. Mutation of HOS15 induces histone hyperacetylation and methylation changes similar to hda9 and pwr mutants. HOS15, HDA9, and PWR are coexpressed in all organs, and mutant combinations display remarkable phenotypic resemblance and nonadditivity for organogenesis and developmental phase transitions. Ninety percent of HOS15-regulated genes are also controlled by HDA9 and PWR. HDA9 binds to and directly represses 92 genes, many of which are responsive to biotic and abiotic stimuli, including a family of ethylene response factor genes. Additionally, HOS15 regulates HDA9 nuclear accumulation and chromatin association. Collectively, this study establishes that HOS15 forms a core complex with HDA9 and PWR to control gene expression and plant development.

show abstract

“…While plants and animals utilize distinct sets of accessory proteins to recognize methylated H3K27 (10,(16)(17)(18), they are generally thought to mediate repression in the context of a canonical PRC1 complex (7,(19)(20)(21). In fungal lineages that employ H3K27 methylation as a repressive chromatin mark, however, core PRC1 components are notably absent (7).…”

Section: Introductionmentioning

confidence: 99%

Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing

Wiles

McNaught

Silva

et al. 2019

Preprint

View full text Add to dashboard Cite

AbstractMethylation of histone H3 lysine 27 (H3K27) is widely recognized as a transcriptionally repressive chromatin modification but the mechanism of repression remains unclear. We devised and implemented a forward genetic scheme to identify factors required for H3K27 methylation-mediated silencing in the filamentous fungus Neurospora crassa and identified a bromo-adjacent homology (BAH)-plant homeodomain (PHD)-containing protein, EPR-1 (Effector of Polycomb Repression 1; NCU07505). EPR-1 associates with H3K27 methylation in vivo and in vitro, and loss of EPR-1 de-represses H3K27-methylated genes without loss of H3K27 methylation. EPR-1 is not fungal-specific; orthologs of EPR-1 are present in a diverse array of eukaryotic lineages, suggesting an ancestral EPR-1 was a component of a primitive Polycomb repression pathway.SignificancePolycomb group (PcG) proteins are employed by a wide variety of eukaryotes for the maintenance of gene repression. Polycomb repressive complex 2 (PRC2), a multimeric complex of PcG proteins, catalyzes the methylation of histone H3 lysine 27 (H3K27). In the filamentous fungus, Neurospora crassa, H3K27 methylation represses scores of genes, despite the absence of canonical H3K27 methylation effectors that are present in plants and animals. We report the identification and characterization of an H3K27 methylation effector, EPR-1, in N. crassa and demonstrate its widespread presence and early eukaryotic origins with phylogenetic analyses. These findings indicate that an ancient EPR-1 may have been part of a nascent Polycomb repression system in eukaryotes.

show abstract

EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis

Cited by 102 publications

References 50 publications

Epigenetic regulation in plant abiotic stress responses

Epigenetic regulation in plant abiotic stress responses

HDA9-PWR-HOS15 Is a Core Histone Deacetylase Complex Regulating Transcription and Development

Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing

Contact Info

Product

Resources

About