Ebp1 protein expression in cervical cancer tissue and its significance

Liu, L; Xu, Dongmei; Yang, Shasha; Li, X D

doi:10.4238/2015.may.22.20

Cited by 7 publications

(9 citation statements)

References 7 publications

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“…EBP1 was originally identified as an ErbB3 binding protein which was considered as an oncogene or tumor suppressor in different circumstances (38–41) and has prognostic significance in a panel of cancers such as cervical (7, 42), pancreatic (6), hepatocellular (43), brain (44) and other tumors. Recent study has clarified that EBP1 possesses two isoforms, the longer form P48 and the shorter form P42, which distinctively regulate cell survival and differentiation (5).…”

Section: Discussionmentioning

confidence: 99%

Distinct Interactions of EBP1 Isoforms with FBXW7 Elicits Different Functions in Cancer

et al. 2017

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The ErbB3 receptor binding protein EBP1 encodes two alternatively spliced isoforms p48 and p42. While there is evidence of differential roles for these isoforms in tumorigenesis, little is known about their underlying mechanisms. Here we demonstrate that EBP1 isoforms interact with the SCF-type ubiquitin ligase FBXW7 in distinct ways to exert opposing roles in tumorigenesis. EBP1 p48 bound to the WD domain of FBXW7 as an oncogenic substrate of FBXW7. EBP1 p48 binding sequestered FBXW7α to the cytosol, modulating its role in protein degradation and attenuating its tumor suppressor function. In contrast, EBP1 p42 bound to both the F-box domain of FBXW7 as well as FBXW7 substrates. This adapter function of EBP1 p42 stabilized the interaction of FBXW7 with its substrates and promoted FBXW7-mediated degradation of oncogenic targets, enhancing its overall tumor suppressing function. Overall, our results establish distinct physical and functional interactions between FBXW7 and EBP1 isoforms which yield their mechanistically unique isoform-specific functions of EBP1 in cancer.

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Section: Discussionmentioning

confidence: 99%

Distinct Interactions of EBP1 Isoforms with FBXW7 Elicits Different Functions in Cancer

et al. 2017

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“…Basically, HPV types are classified in two important groups based on oncogenic potential: the high-risk (HR) and low-risk (LR). The HR types, constituted by types 16,18,31,33,35,39,45,51,52,56,58,59, among others, are found in 99% of invasive cancer cases, like anogenital and oropharyngeal cancers, with types 16 and 18 being the most representative, which induce cervical lesions 25 . LR The human papillomavirus E6 and E7 oncoproteins have affinity for two important regulatory proteins, p53 and Rb, respectively, and this ability does not allow these proteins to perform their correct functions.…”

Section: Hpvmentioning

confidence: 99%

“…This test distinguishes between the HR and LR groups and detects 13 HR-HPV types and 5 LR types, but it is not a sensitive for the genotyping of a single virus (51 type). The Cervista® HPV detects the presence of 14 high-risk types (16,18,31,33,35,39,45,51,52,56,58,59, 66 and 68) 29 . These assays showed lower false-positive rates and high sensitivity and specificity for HPV-16/18 genotyping 30 .…”

Section: Nucleic Acid Hybridization Assaysmentioning

confidence: 99%

“…Multiplex PCR, such as PapilloCheck® assay, detects the genotypes of 24 HPV types in a single reaction 11,16,18,31,33,35,39,40,42,43,44,45,51,52,53,55,56,58,59,66,68,70,73 and 82). The assay uses fluorescent primers to amplify a 350 bp fragment of the HPV E1 gene, comprising 28 probes, each at five replicate points fixed on a DNA chip 37 .…”

Section: Polymerase Chain Reaction Techniquesmentioning

confidence: 99%

“…For this reason, the search for E6/E7 transcripts could increase the specificity and sensitivity of screening tests for cervical lesions, that is more specific than HPV-PCR for the detection of HSIL (high-grade squamous intraepithelial lesions) 28 . PreTect® Proofer has a high ability to detect five HR-HPV types (16, 18, 31, 33 and 45 HPV types) 28 and APTIMA® HPV assay has a greater sensitivity to detect 14 HR-HPV types (16,18,31,33,35,39,45,51,52,56,58,59, 66 and 68) 46 . These assays have several advantages over the other HPV tests and may be an excellent marker of cervical cancer.…”

Section: Polymerase Chain Reaction Techniquesmentioning

confidence: 99%

See 2 more Smart Citations

Potential Diagnostic Techniques for Cervical Cancer Prevention - Review

Melo¹,

Ribeiro²,

Canevari³

2018

J Cancer Treat & Diagnosis

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Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and several studies have proven the close relationship between HPV and the development of cervical cancer. Several tests are currently performed for early and reliable diagnosis, in which the cervical cytology evaluation by the Papanicolaou method is highlighted. However, errors in the collection and misinterpretation of cell differentiation degree in smear by the pathologist result in incorrect or inaccurate results. Considering these points, it is of utmost importance to develop new technologies that perform accurate and reliable diagnosis and that present financial advantages and accessibility. Currently, molecular biology assays are excellent detectors of viral DNA, but it presents disadvantages, such as the need for high financial resources to gain access. Therefore, this review has the objective of highlighting the main diagnostic techniques that are already being used, such as specific kits for the detection of high-risk HPVs, or methodologies that are still in the study phase, but which already present good results, such as the application of physical FTIR spectroscopy principles and ultrasensitive biosensors.

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Identification of diagnostic biomarkers via weighted correlation network analysis in colorectal cancer using a system biology approach

Ghafouri-Fard,

Safarzadeh,

Taheri

et al. 2023

Sci Rep

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Colorectal cancer (CRC) is the third most frequent cancer to be diagnosed in both females and males necessitating identification of effective biomarkers. An in-silico system biology approach called weighted gene co-expression network analysis (WGCNA) can be used to examine gene expression in a complicated network of regulatory genes. In the current study, the co-expression network of DEGs connected to CRC and their target genes was built using the WGCNA algorithm. GO and KEGG pathway analysis were carried out to learn more about the biological role of the DEmRNAs. These findings revealed that the genes were mostly enriched in the biological processes that were involved in the regulation of hormone levels, extracellular matrix organization, and extracellular structure organization. The intersection of genes between hub genes and DEmRNAs showed that DKC1, PA2G4, LYAR and NOLC1 were the clinically final hub genes of CRC.

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Ebp1 protein expression in cervical cancer tissue and its significance

Cited by 7 publications

References 7 publications

Distinct Interactions of EBP1 Isoforms with FBXW7 Elicits Different Functions in Cancer

Distinct Interactions of EBP1 Isoforms with FBXW7 Elicits Different Functions in Cancer

Potential Diagnostic Techniques for Cervical Cancer Prevention - Review

Identification of diagnostic biomarkers via weighted correlation network analysis in colorectal cancer using a system biology approach

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