Easy pan-detection of human IgA immunoglobulins

Planchais, Cyril; Mouquet, Hugo

doi:10.1016/j.jim.2020.112833

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…The levels of immunoglobulin in animal blood directly reflect the immune activity of animals. Previous studies found that Lycium barbarum residue can increase the levels of IgA and IgG in serum of Tibetan sheep, thereby improving immunity (31,32). Similarly, our results also showed that fermented Lycium barbarum could promote the levels of IgA and IgG of sheep.…”

Section: The Effects Of Rfw On Iga and Igg Of Sheepsupporting

confidence: 86%

“…Various studies have shown that the increases in immunoglobulin in vivo is mainly caused by the high levels of Lycium barbarum polysaccharide (1,31). Our study showed that RFW-induced multiple carbohydrates are involved in the network of immune-related genes and metabolites, such as cellobiose, Beta-D-galactose and trehalose.…”

Section: The Effects Of Rfw On Acting Immune-related Genesmentioning

confidence: 51%

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Integrated Metabolomics and Transcriptome Revealed the Effect of Fermented Lycium barbarum Residue Promoting Ovis aries Immunity

et al. 2022

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Lycium barbarum residue contains abundant bioactive nutrients which can be used as feed supplement. The fermentation treatment of plant residue can promote the utilization of nutrients, rumen digestion, and the growth and immunity of animals. Based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabolomics and in-depth transcriptome analysis, the study tested the mechanisms of Lycium barbarum residue (RW) and fermented Lycium barbarum residue (RFW) on meat quality and immunity of sheep. Fifty-four Tan sheep were randomly divided into control, RFW or RW treatments. Data showed that RFW and RW increased the carcass weight, fat content, ash content and reduced the cooking loss of lamb. RFW performed more significant effects on activating immune-related genes than those of RW. The expression of chemokines and immune-related pathways, such as signaling pathways of interleukin-17 signaling pathway and NOD-like receptor signaling pathway, were elevated in sheep fed RFW. RW increased the diversity in rumen metabolites, especially compositions of lipids, organic acids and organ heterocyclic compounds. RFW affected numerous compounds which are closely correlated with the activation of immune genes. In conclusion, RFW could represent a valuable strategy to improve growth performance and immunity of sheep.

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Section: The Effects Of Rfw On Iga and Igg Of Sheepsupporting

confidence: 86%

Section: The Effects Of Rfw On Acting Immune-related Genesmentioning

confidence: 51%

Integrated Metabolomics and Transcriptome Revealed the Effect of Fermented Lycium barbarum Residue Promoting Ovis aries Immunity

et al. 2022

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“…Immunoglobulins reflect the immune status of the animal (Yuan et al, 2015;. Hao et al (2015) reported that the major serum Igs, IgA, IgG, and IgM, were key humoral immunity components in all mammals; they enhance monocyte macrophage phagocytosis and inhibit pathogenic virus and microorganism reproduction (Heidebrecht and Kulozik, 2019;Planchais and Mouquet, 2020). In our study, dietary LBPs supplementation increased serum IgG and IgM levels in weaned piglets.…”

Section: Discussionsupporting

confidence: 48%

Lycium barbarum Polysaccharides as Antibiotic Substitutes Improve Growth Performance, Serum Immunity, Antioxidant Status, and Intestinal Health for Weaned Piglets

et al. 2022

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The aim of the present study is to investigate the effects of dietary Lycium barbarum polysaccharides (LBPs) supplementation on the growth performance, immune response, serum antioxidant status, and intestinal health of weaned piglets. In total, 24 crossed healthy weaned piglets [Duroc × (Yorkshire × Landrace)], of similar body weight (7.47 ± 0.22 kg), were randomly allocated to three treatment groups: CON (basal diet); LBPs (basal diet plus 4,000 mg/kg LBPs); and antibiotic (ABO, basal diet plus 20 mg/kg flavomycin and 50 mg/kg quinocetone). There were eight pigs per group. The study lasted 28 days. When compared with CON, LBPs or ABO dietary supplementation increased average daily gain (P < 0.05), decreased the ratio of feed to gain and the diarrhea ratio (P < 0.05). Similarly, when compared with CON, LBPs dietary supplementation increased serum immunoglobulin G, immunoglobulin M, interleukin-10, interleukin-2, and tumor necrosis factor-α levels (P < 0.05). Dietary LBPs enhanced the activity of serum total antioxidant capacity and glutathione peroxidase, and decreased malondialdehyde levels (P < 0.05). Principal component analysis showed a distinct separation between CON and LBPs groups, but no differences between ABO and LBPs groups. LBPs addition increased Lactobacillus and Faecalibacterium (P < 0.05) levels, while it decreased Enterococcaceae and Enterobacteriaceae (P < 0.05) levels. Furthermore, when compared with the CON group, LBPs increased villus height (P < 0.05) and the villus height to crypt depth ratio in the duodenum and jejunum (P < 0.05). Thus, dietary supplementation with LBPs improved growth performance, antioxidant capacity and immunity, regulated intestinal microbial composition, and may be used as an efficient antibiotic alternative in weaned piglet feed.

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“…IgM antibodies are found mostly in circulation where they can facilitate antigen opsonization (26). Recent studies have also revealed diverse roles for secretory IgM in the mucosa of the gastrointestinal and respiratory tracts (27). Human IgA immunoglobulins, which can be further subdivided into the IgA1 and IgA2 subclasses (28), generally exceed levels of IgM in serum and are significantly more present in mucosal surfaces and secretions (i.e., saliva, breast milk, etc.)…”

Section: Down To the Basics: Antibody Classes And Class Switchingmentioning

confidence: 99%

Humoral Responses and Serological Assays in SARS-CoV-2 Infections

et al. 2020

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In December 2019, the novel betacoronavirus Severe Acute Respiratory Disease Coronavirus 2 (SARS-CoV-2) was first detected in Wuhan, China. SARS-CoV-2 has since become a pandemic virus resulting in hundreds of thousands of deaths and deep socioeconomic implications worldwide. In recent months, efforts have been directed towards detecting, tracking, and better understanding human humoral responses to SARS-CoV-2 infection. It has become critical to develop robust and reliable serological assays to characterize the abundance, neutralization efficiency, and duration of antibodies in virus-exposed individuals. Here we review the latest knowledge on humoral immune responses to SARS-CoV-2 infection, along with the benefits and limitations of currently available commercial and laboratory-based serological assays. We also highlight important serological considerations, such as antibody expression levels, stability and neutralization dynamics, as well as cross-reactivity and possible immunological back-boosting by seasonal coronaviruses. The ability to accurately detect, measure and characterize the various antibodies specific to SARS-CoV-2 is necessary for vaccine development, manage risk and exposure for healthcare and at-risk workers, and for monitoring reinfections with genetic variants and new strains of the virus. Having a thorough understanding of the benefits and cautions of standardized serological testing at a community level remains critically important in the design and implementation of future vaccination campaigns, epidemiological models of immunity, and public health measures that rely heavily on up-to-date knowledge of transmission dynamics.

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Easy pan-detection of human IgA immunoglobulins

Cited by 9 publications

References 21 publications

Integrated Metabolomics and Transcriptome Revealed the Effect of Fermented Lycium barbarum Residue Promoting Ovis aries Immunity

Integrated Metabolomics and Transcriptome Revealed the Effect of Fermented Lycium barbarum Residue Promoting Ovis aries Immunity

Lycium barbarum Polysaccharides as Antibiotic Substitutes Improve Growth Performance, Serum Immunity, Antioxidant Status, and Intestinal Health for Weaned Piglets

Humoral Responses and Serological Assays in SARS-CoV-2 Infections

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