Easy and inexpensive molecular detection of dengue, chikungunya and zika viruses in febrile patients

Improved detection of Zika virus RNA in human and animal specimens by a novel, highly sensitive and specific real‐time RT‐PCR assay targeting the 5′‐untranslated region of Zika virus

“…The performance of ZIKV‐5′‐UTR for the detection of ZIKV RNA in patient's urine and sperm specimens should be further evaluated in clinical studies, as these are important secretions involved in the non‐vector‐borne transmission of ZIKV. Development of ZIKV 5′‐UTR into a multiplex assay may further increase its clinical utility .…”

Descripción de pruebas moleculares en el diagnóstico del virus Zika en el periodo 2008-Febrero 2018. Revisión sistemática

“…Por otra parte, al identificar los componentes moleculares que permiten la detección del ZIKV, aun cuando en cada una de las técnicas utilizadas hay una gran variabilidad en cuanto al blanco molecular de interés, se determinó que los principales blancos moleculares son los genes E, Ns1 y Ns5, siendo el gen E el de mayor predominio y de uso común en todas las técnicas ya que es el gen encargado de la adherencia y fusión con la membrana. (Ver Tabla1) (15,34,50).…”

Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system

“…Ideally, the arbo‐PCR multiplex also includes WNV. Recently, three multiplex assays targeting ZIKV, DENV, and CHIKV have been published, including one released by the Centers for Disease Control and Prevention under an emergency authorization . None of the three multiplex PCRs was evaluated with the cobas omni UC reagents on the cobas 6800 system.…”

“…Recently, three multiplex assays targeting ZIKV, DENV, and CHIKV have been published, including one released by the Centers for Disease Control and Prevention under an emergency authorization. [35][36][37] None of the three multiplex PCRs was evaluated with the cobas omni UC reagents on the cobas 6800 system. Partially or complete adoption and/or modification of the primer sets used by one of three published multiplex PCRs could be an alternative for a new primer design covering DENV and CHIKV.…”