Easing diagnosis and pushing the detection limits of SARS-CoV-2

Kiran, Uday; Gokulan, C G; Kuncha, Santosh Kumar; Vedagiri, Dhiviya; Bingi, Thrilok Chander; Sekhar, Aedula Vinaya; Dontamala, Suchitra; Reddy, Arakatla Lohith; Tallapaka, Karthik Bharadwaj; Mishra, Rakesh K.; Harshan, Krishnan Harinivas

doi:10.1093/biomethods/bpaa017

Cited by 17 publications

(33 citation statements)

References 12 publications

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“…RT-PCR being laborious, time-consuming, requiring high-end equipment and trained manpower, it’s been of limited use in the resource-constrained settings. However, a recent study has shown the use of direct swab sample which will further simplify the test by eliminating the RNA isolation step ( Kiran et al, 2020 ).…”

Section: Covid-19 Diagnosticsmentioning

confidence: 99%

Wuhan to World: The COVID-19 Pandemic

Kumar

Singh

Kaur

et al. 2021

Front. Cell. Infect. Microbiol.

167

111

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COVID-19 is a Severe Acute Respiratory Syndrome (SARS), caused by SARS-CoV-2, a novel virus which belongs to the family Coronaviridae. It was first reported in December 2019 in the Wuhan city of China and soon after, the virus and hence the disease got spread to the entire world. As of February 26, 2021, SARS-CoV-2 has infected ~112.20 million people and caused ~2.49 million deaths across the globe. Although the case fatality rate among SARS-CoV-2 patient is lower (~2.15%) than its earlier relatives, SARS-CoV (~9.5%) and MERS-CoV (~34.4%), the SARS-CoV-2 has been observed to be more infectious and caused higher morbidity and mortality worldwide. As of now, only the knowledge regarding potential transmission routes and the rapidly developed diagnostics has been guiding the world for managing the disease indicating an immediate need for a detailed understanding of the pathogen and the disease-biology. Over a very short period of time, researchers have generated a lot of information in unprecedented ways in the key areas, including viral entry into the host, dominant mutation, potential transmission routes, diagnostic targets and their detection assays, potential therapeutic targets and drug molecules for inhibiting viral entry and/or its replication in the host including cross-neutralizing antibodies and vaccine candidates that could help us to combat the ongoing COVID-19 pandemic. In the current review, we have summarized the available knowledge about the pathogen and the disease, COVID-19. We believe that this readily available knowledge base would serve as a valuable resource to the scientific and clinical community and may help in faster development of the solution to combat the disease.

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Section: Covid-19 Diagnosticsmentioning

confidence: 99%

Wuhan to World: The COVID-19 Pandemic

Kumar

Singh

Kaur

et al. 2021

Front. Cell. Infect. Microbiol.

167

111

View full text Add to dashboard Cite

show abstract

“…Recently, RNA extraction from the swab samples was shown to be dispensable thereby enabling direct processing of the samples for qRT-PCR in COVID-19 screening [14] as well as in other respiratory viral infections [23, 24]. Collecting the samples in dry-swab form has been demonstrated to be effective in preserving the viral content and also much more bio-safe [23, 24].…”

Section: Resultsmentioning

confidence: 99%

“…The supernatants were titrated for infectious particle count by plaque-forming assay. In the case of dry-swab sample, the swab was first soaked in TE buffer for 30 minutes [14] and further stored at −80°C freezers. Later the sample was used as inoculum for infection similar to VTM.…”

Section: Methodsmentioning

confidence: 99%

Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential

Gupta

Parthasarathy

Sah

et al. 2021

Preprint

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Inactivated viral preparations are important resources in vaccine and antisera industry. Of the many vaccines that are being developed against COVID-19, inactivated whole-virus vaccines are also considered effective. β-propiolactone (BPL) is a widely used chemical inactivator of several viruses. Here, we analyze various concentrations of BPL to effectively inactivate SARS-CoV-2 and their effects on the biochemical properties of the virion particles. BPL at 1:2000 (v/v) concentrations effectively inactivated SARS-CoV-2. However, higher BPL concentrations resulted in the loss of both protein content as well as the antigenic integrity of the structural proteins. Higher concentrations also caused substantial aggregation of the virion particles possibly causing undesirable outcomes including a potential immune escape by infectious virions, and a loss in antigenic potential. We also identify that the viral RNA content in the culture supernatants can be a direct indicator of their antigenic content. Our findings may have important implications in the vaccine and antisera industry during COVID-19 pandemic.

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“…(a) One-step PCR: CSIR-CCMB has developed a one-step RTqPCR reaction with dry swab samples (Figure 1) [17]. The testing centres can send samples without VTM, and thus, the safety and cost of packing and unpacking samples at the sample collection and testing centres can be improved.…”

Section: Optimisation Of Testing Proceduresmentioning

confidence: 99%

Testing and surveillance strategies in the context of COVID-19 in India

Karak

Srivastava

Mishra

2020

Indian Chemical Engineer

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BACKGROUND The COVID-19 pandemic has proceeded unabated across the globe over the last six months and, despite global efforts, experts predict its effects will be felt well into the next year as well. India had its first reported case from Kerala on the same day that the WHO declared the outbreak a Public Health Emergency of International Concern, on 30th January 2020. The government initiated a slew of preventive measures over the next couple of months, culminating in a country-wide lockdown on the 24th of March to help healthcare professionals, policymakers and researchers shore up their resources and devise strategies to fight the infection. Early diagnosis and treatment are critical in any disease but prevention measures that can 'flatten the curve' have proved to be the most effective in managing COVID-19 worldwide. In this article, we discuss key aspects of the disease from the Indian perspective, as well as the new research and development initiatives around COVID-19 and for broader public health.

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Easing diagnosis and pushing the detection limits of SARS-CoV-2

Cited by 17 publications

References 12 publications

Wuhan to World: The COVID-19 Pandemic

Wuhan to World: The COVID-19 Pandemic

Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential

Testing and surveillance strategies in the context of COVID-19 in India

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