Earthworm metallothionein production as biomarker of heavy metal pollution in abattoir soil

Dedeke, G. A.; Owagboriaye, Folarin; Adebambo, A.O.; Ademolu, K. O.

doi:10.1016/j.apsoil.2016.02.013

Cited by 38 publications

(18 citation statements)

References 17 publications

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“…Higher levels of target pollutants in soil have resulted in the clear promotion of MT content although positive correlations between MT content and DEHP concentration in all earthworms have not always been observed. MTs have been widely accepted as efficient biomarkers for monitoring soil contamination, especially for metals and metalloids and they play a vital role in the detoxification pathway of pollutants [38]. However, in the present study substantial promotion of MT content has been detected.…”

Section: Resultscontrasting

confidence: 60%

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Zhou

Chen

et al. 2017

PLoS ONE

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Di-(2-ethylhexyl) phthalate (DEHP) is a dominant phthalic acid ester (PAE) that has aroused public concern due to its resistance to degradation and its toxicity as an endocrine-disrupting compound. Effects of different concentrations of DEHP on Eisenia fetida in spiked natural soil have been studied in the body of the earthworm by means of soil cultivation tests 7, 14, 21 and 28 days after exposure. The results indicated that, in general, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, metallothionein (MT) content, the expression of heat shock protein 70 (HSP 70) and all the tested geno-toxicity parameters are promoted as time elapses and with increasing concentration of DEHP. However, peroxidase (POD) activity, neutral red retention time (NRRT) and mitochondrial membrane potential difference values were found to decrease even at a low concentration of DEHP of 1 mg kg-1 soil (p<0.05). Clear toxic effects of DEHP on E. fetida have been generally recognized by means of the disturbance of antioxidant enzyme activity/content and critical proteins, cell membrane and organelle disorder and DNA damage estimated by length of tail, tail DNA ratio, and tail moment parameters. A concentration of DEHP of 3 mg kg-1 may be recommended as a precaution against the potential risk of PAEs in soils and for indicating suitable threshold values for other soil animals and soil micro-organisms.

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Section: Resultscontrasting

confidence: 60%

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Zhou

Chen

et al. 2017

PLoS ONE

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“…On the other hand, a lower activity of MT in the exposed earthworm species may suggest ability of the earthworm species to adapt and survive in the soil containing this herbicide with lower oxidative stress. MTs are important metal binding proteins in the cells of organisms (Amiard et al, 2006) and studies have measured the activity of MT in earthworm as a potentially useful biomarker of metal pollution in soil (Ma et al, 2008;Wang et al, 2013;Dedeke et al, 2016). To the best of our knowledge, there is paucity of available information on the use of MT activity as a biomarker of GBH ecotoxicity assessment.…”

Section: Activities Of Ldhmentioning

confidence: 99%

“…To this effect, earthworms have been used in ecotoxicological risks monitoring and as bio-indicator of lethal and non-lethal pollutants including heavy metals in environments (Georgescu et al, 2011), mine site (Honsi et al, 2003) and sawmill soil (Bamidele et al, 2015). In our previous studies, we used earthworms to monitor the level of pollution in abattoir soil (Owagboriaye et al, 2016;Dedeke et al, 2016). Earthworms have been used to evaluate organophosphates pollution in soil (O'Halloran et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, we also assessed the vermiremediation potential of the earthworm species in the contaminated soil in order to mitigate the potential impact of the herbicide. These earthworm species are native to Africa and commonly found in the soils of agricultural, industrial and residential areas in Nigeria and have been proven to be a good indicator of environmental pollution in sawmills (Bamidele et al, 2015; and abattoir area (Owagboriaye et al, 2016;Dedeke et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical response and vermiremediation assessment of three earthworm species (Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus) in soil contaminated with a glyphosate-based herbicide

Owagboriaye

Dedeke

Bamidele

et al. 2020

Ecological Indicators

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The global use of glyphosate-based herbicides (GBHs) and release of glyphosate residues in soil environment have over the years been a major concern. In this study, we aim to evaluate the biochemical response and vermiremediation potential of three indigenous earthworm species; namely Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus in GBH treated soils. Study design: Three weed plants (Tridax procumbense, Ludwigia pasturis and Pannicum maximum) were transplanted into 140 plastic pots and 20 adult individual earthworms of each species were later introduced into 60 pots. In total, each earthworm species treatments representing 20 pots and 20 pots without earthworm were sprayed with 115.49 mL/m 2 (equivalent to 83.2 g a.i/m 2) of Roundup® Alphée. The remaining 60 pots with earthworm species were left unsprayed. Activities of glutathione-S-transferase (GST), lactate dehydrogenase (LDH), metallothionine (MT), acetylcholinesterase (AChE), antioxidant defense system and lipid peroxidation (MDA) were monitored at the 1st, 2nd, 4th, 6th and 8th weeks Post Herbicide Application (PHA) using standard methods. Glyphosate residues in the soil and earthworm species were quantified with a High-Performance Liquid Chromatography (HPLC) with fluorescence detector. Bioaccumulation Factor (BAF) was also calculated. Results: Higher activities of GST and LDH and reduction in MT activities were observed in the three earthworm species exposed to GBH compared to the unexposed while AChE activity was insensitive to the herbicide. The antioxidant defence system was able to protect E. eugeniae and L. violaceus against oxidative stress. The presence of earthworms reduced glyphosate residues in the soil. E. eugeniae and L. violaceus were bioaccumalators and biomagnifiers of glyphosate as indicated by the BAF (> 1) obtained after 8th week PHA. Relationships exist between glyphosate BAF in E. eugeniae and L. violaceus and exposure duration as well as between glyphosate residues in tissues of the earthworm species and their biochemical parameters. Conclusions: Alterations in the enzymatic activities and antioxidant defence of the earthworm species could be an index for GBH contaminated soil monitoring and assessment; both E. eugeniae and L. violaceus showed potential to vermiremediate soils contaminated with GBH.

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“…Since the reporter gene was expressed in DKO7 cells, we conclude that the promoter activity is not dependent on mMTF1. Furthermore, we suggest that the additional distal promoter regions in insert 1-3 cause a repression of the consecutive activation, which makes sense, considering that MT genes have to be expressed rapidly under stressful conditions and show very low expression levels in controls [39,41]. The activation of genes in response to stress can be manifold-either way, cells aim at changing their transcriptional landscape within minutes in response to stress [42].…”

Section: Discussionmentioning

confidence: 99%

Promoter activity of earthworm metallothionein in mouse embryonic fibroblasts

2019

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The regulation of metallothionein (MT) gene expression as important part of the detoxification machinery is only scarcely known in invertebrates. In vertebrates, MT gene activation is mediated by the metal-transcription factor 1 (MTF-1) binding to metal response elements (MREs). In invertebrates, the mechanisms of MT gene activation seems to be more diverse. In some invertebrate species, MTF-1 orthologues as well as their ability to activate MT genes via MREs have been uncovered. Although earthworm MTs have been well studied, a MTF-1 orthologue has not yet been described and MT gene activation mechanisms are largely unknown. Analyses of the earthworm wMT2 promoter by reporter gene assays have been performed. We could show that the wMT2 promoter was active in mouse embryonic fibroblasts (NIH/3T3) as well as in mouse MTF-1 −/− cells (DKO7). The presence of mouse MTF-1 (mMTF1) led to a significant increase in reporter gene activity. We observed that cadmium as well as zinc had an effect on promoter activity. In the presence of zinc, promoter activity doubled in NIH cells, however, we did not observe a significant effect in the DKO7 cell line. Cadmium decreased promoter activity in DKO7 cells, but this effect could be reversed by providing mMTF1 in a co-transfection experiment. We suggest that MT gene expression in the earthworm is not entirely dependent on a MRE binding protein. Interestingly, the shortest promoter fragment including MRE1 showed the highest promoter activity under control conditions.

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Earthworm metallothionein production as biomarker of heavy metal pollution in abattoir soil

Cited by 38 publications

References 17 publications

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Biochemical response and vermiremediation assessment of three earthworm species (Alma millsoni, Eudrilus eugeniae and Libyodrilus violaceus) in soil contaminated with a glyphosate-based herbicide

Promoter activity of earthworm metallothionein in mouse embryonic fibroblasts

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