An extraskeletal osteosarcoma was detected in the auricle of a 110-week-old female
Wistar Hannover rat. Grossly, the tumor, measuring 15 mm in size, was observed in the
subcutis as a solid and hard mass. Histologically, the majority of the mass comprised
mature, compact bone. It was surrounded by neoplastic cells showing a variety of
histologies, such as sarcoma, not otherwise specified, and myxosarcoma away from the
bone-forming region. However, these different histological regions were considered to be
components of a single bone tumor, based on the common expression of osterix and a similar
mixture of constituent cells in each region. The tumor was diagnosed as an extraskeletal
osteosarcoma because of the presence of infiltrative growth and abnormal mitosis and its
development in the auricle without attachment to the skeleton. The present case is a rare
histological type of an extraskeletal osteosarcoma with independent and different
histological elements in rats.