Early Supplementation of Phospholipids and Gangliosides Affects Brain and Cognitive Development in Neonatal Piglets

Liu, Hongnan; Radlowski, Emily C.; Conrad, Matthew S.; Yao, Li; Dilger, Ryan N.; Johnson, Rodney W.

doi:10.3945/jn.114.199828

Cited by 93 publications

(73 citation statements)

References 38 publications

(51 reference statements)

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“…Previous studies in new‐born piglets have found no difference in cognitive function and total brain volume between piglets fed with milk containing different supplements . At birth, the piglets in our study were randomised to either parenteral nutrition or parenteral nutrition in combination with small amounts of bovine colostrum, and as we previously found no difference in later outcomes according to these diets , the data were analysed according to term or preterm delivery.…”

Section: Discussionmentioning

confidence: 84%

Structural brain maturation differs between preterm and term piglets, whereas brain activity does not

Plomgaard

Andersen²,

Petersen

et al. 2018

Acta Paediatrica

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Functional brain maturation, as assessed by aEEG, was relatively advanced in preterm piglets. Conversely, the low FA in the preterm piglets suggests that the white matter microstructure remains less mature in preterm compared to term piglets at postnatal day 25. The results might be utilised to define whether and how preterm piglets may contribute to preclinical models for brain development in preterm infants.

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Section: Discussionmentioning

confidence: 84%

Structural brain maturation differs between preterm and term piglets, whereas brain activity does not

Plomgaard

Andersen²,

Petersen

et al. 2018

Acta Paediatrica

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“…Lipids have many biological roles, from roles in brain function and development (Hellhammer et al, 2010;Nagai, 2012;Liu et al, 2014), to roles in cell cycle regulation and cell signaling (Garcia-Gil et al, 2017;Ng et al, 2017). Additionally, lipids are key components of membranes.…”

Section: Lipid Imbalance and Its Impactmentioning

confidence: 99%

Yeast membrane lipid imbalance leads to trafficking defects toward the Golgi

Woodman

Trousdale

Conover

et al. 2018

Cell Biology International

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Protein recycling is an essential cellular process involving endocytosis, intracellular trafficking, and exocytosis. In mammalian systems membrane lipids, including cholesterol, sphingolipids, and phospholipids, play a pivotal role in protein recycling. To address this role in budding yeast, Saccharomyces cerevisiae, we utilized GFP-Snc1, a v-SNARE protein serving as a fluorescent marker for faithfully reporting the recycling pathway. Here we demonstrate results that display moderate to significant GFP-Snc1 recycling defects upon overexpression or inactivation of phospholipid, ergosterol, and sphingolipid biosynthesis enzymes, indicating that the homeostasis of membrane lipid levels is prerequisite for proper protein recycling. By using a truncated version of GFP-Snc1 that cannot be recycled from the plasma membrane, we determined that abnormalities in Snc1 localization in membrane lipid overexpression or underexpression mutants are not due to defects in the synthetic/secretory pathway, but rather in the intracellular trafficking pathway. We found that membrane lipid imbalance resulted in an accumulation of the late endosome marker Vps10-GFP, indicating trafficking from the endosomes to the Golgi may be being hindered, preventing recycling to the plasma membrane. To elucidate the possible mechanism for this trafficking hindrance, we stained the actin cytoskeleton, then quantified the percentage of cells with visible actin cables. Compared to wild-type cells, membrane lipid mutant cells exhibited lower levels of actin cables, indicating the actin cytoskeleton is disrupted upon membrane lipid imbalance. Taken together, our results show that impairment of proper recycling may be due to disruption of the actin cytoskeleton, which causes trafficking hindrance between the endosomes and Golgi.

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“…Complex milk lipids (CML), as a source of GA, have potential benefits for brain development. [19][20][21] The rate of GA accretion in the developing brain is highest in utero and in early neonatal life. 22 23 Preclinical research has shown that CML are transferred across the placenta and as such, an increased maternal intake of CML in pregnancy has been associated with increases in fetal GA levels.…”

Section: Introductionmentioning

confidence: 99%

The CLIMB (Complex Lipids In Mothers and Babies) study: protocol for a multicentre, three-group, parallel randomised controlled trial to investigate the effect of supplementation of complex lipids in pregnancy, on maternal ganglioside status and subsequent cognitive outcomes in the offspring

Huang

Norris

et al. 2017

BMJ Open

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IntroductionComplex lipids are important constituents of the central nervous system. Studies have shown that supplementation with complex milk lipids (CML) in pregnancy may increase the level of fetal gangliosides (GA), with the potential to improve cognitive outcomes.Methods and analysisWe aim to recruit approximately 1500 pregnant women in the first trimester (11–14 weeks) and randomise them into one of the three treatment groups: standard maternal milk formulation, CML-enhanced maternal milk formulation or no maternal milk intervention with standard pregnancy advice (ie, the standard care). Maternal lifestyle and demographic data will be collected throughout the pregnancy, as well as biological samples (eg, blood, hair, urine, buccal smear, cord blood, cord and placenta samples). Data from standard obstetric care recorded in hospital maternity notes (eg, ultrasound reports, results of oral glucose tolerance test and pregnancy outcome data) will also be extracted. Postnatal follow-up will be at 6 weeks and 12 months of age, at which point infant cognitive development will be assessed (Bayley Scales of Infant Development I).Ethics and disseminationThis project was approved by the Ethics Committee of Chongqing Medical University. Dissemination of findings will take the form of publications in peer-reviewed journals and presentations at national and international conferences.Trial registration numberChiCTR-IOR-16007700; Pre-results.

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Early Supplementation of Phospholipids and Gangliosides Affects Brain and Cognitive Development in Neonatal Piglets

Abstract: In summary, dietary phospholipids and gangliosides improved spatial learning and affected brain growth and composition in neonatal piglets.

Cited by 93 publications

References 38 publications

Structural brain maturation differs between preterm and term piglets, whereas brain activity does not

Structural brain maturation differs between preterm and term piglets, whereas brain activity does not

Yeast membrane lipid imbalance leads to trafficking defects toward the Golgi

The CLIMB (Complex Lipids In Mothers and Babies) study: protocol for a multicentre, three-group, parallel randomised controlled trial to investigate the effect of supplementation of complex lipids in pregnancy, on maternal ganglioside status and subsequent cognitive outcomes in the offspring

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