Early Stages of Antibacterial Damage of Metallic Nanoparticles by TEM and STEM-HAADF

España‐Sánchez, Beatriz Liliana; Ávila‐Orta, Carlos A.; Padilla-Vaca, Luis Felipe; Castro, Emilio Mata de; Soriano‐Corral, Florentino; González‐Morones, Pablo; Wong, Diana Guadalupe Ramirez; Luna-Bárcenas, Gabriel

doi:10.2174/2468187307666170906150731

Cited by 16 publications

(9 citation statements)

References 46 publications

(50 reference statements)

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“…The disintegration of the cell wall and the release of substances were identified through TEM images, so Ag NPs proved to be toxic for both bacteria [ 62 ]. España-Sánchez et al [ 63 ] studied, through the HRTEM technique, the contact realized between spherical Ag NPs and Pseudomonas aeruginosa bacteria. The images proved that Ag NPs agglomerate at the bacterial wall, eventually causing disruption and internalization.…”

Section: Silver Nanoparticles—overview and Synthesis Methodsmentioning

confidence: 99%

“…The images proved that Ag NPs agglomerate at the bacterial wall, eventually causing disruption and internalization. The diffusion of Ag NPs is increased within this penetration so that bacterial death is completed [ 63 ]. This mechanism seems to be more expressed in the case of Gram-negative bacteria.…”

Section: Silver Nanoparticles—overview and Synthesis Methodsmentioning

confidence: 99%

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Ag Nanoparticles for Biomedical Applications—Synthesis and Characterization—A Review

Nicolae-Maranciuc

Chicea

2022

IJMS

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Silver nanoparticles have been intensively studied over a long period of time because they exhibit antibacterial properties in infection treatments, wound healing, or drug delivery systems. The advantages that silver nanoparticles offer regarding the functionalization confer prolonged stability and make them suitable for biomedical applications. Apart from functionalization, silver nanoparticles exhibit various shapes and sizes depending on the conditions used through their fabrications and depending on their final purpose. This paper presents a review of silver nanoparticles with respect to synthesis procedures, including the polluting green synthesis. Currently, the most commonly used characterization techniques required for nanoparticles investigation in antibacterial treatments are described briefly, since silver nanoparticles possess differences in their structure or morphology.

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Section: Silver Nanoparticles—overview and Synthesis Methodsmentioning

confidence: 99%

Section: Silver Nanoparticles—overview and Synthesis Methodsmentioning

confidence: 99%

Ag Nanoparticles for Biomedical Applications—Synthesis and Characterization—A Review

Nicolae-Maranciuc

Chicea

2022

IJMS

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“…Even though some alteration to the structure of the SDSD VSD22 cells after treatment were observed ( Figure 6 ), these did not affect the growth of this strain. Untreated cells exhibited a uniform cytoplasm, while aggregation of the cytoplasmic components was observed among treated cells (see Figure 6C ; España-Sánchez et al, 2017 ). It has been suggested that these cytoplasmic alterations might be associated to some degree of damage to the cell membrane ( Dakal et al, 2016 ; Wang et al, 2016 ).…”

Section: Resultsmentioning

confidence: 94%

Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles

et al. 2022

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Bacterial biofilm is a tri-dimensional complex community of cells at different metabolic stages involved in a matrix of self-produced extracellular polymeric substances. Biofilm formation is part of a defense mechanism that allows the bacteria to survive in hostile environments, such as increasing resistance or tolerance to antimicrobial agents, causing persistent infections hard to treat and impair disease eradication. One such example is bovine mastitis associated with Streptococcus dysgalactiae subsp. dysgalactiae (SDSD), whose worldwide health and economic impact is on the surge. As such, non-conventional nanobased approaches have been proposed as an alternative to tackle biofilm formation and to which pathogenic bacteria fail to adapt. Among these, metallic nanoparticles have gained significant attention, particularly gold and silver nanoparticles, due to their ease of synthesis and impact against microorganism growth. This study provides a proof-of-concept investigation into the use of gold-silver alloy nanoparticles (AuAgNPs) toward eradication of bacterial biofilms. Upon visible light irradiation of AuAgNPs there was considerable disturbance of the biofilms’ matrix. The hindering of structural integrity of the biofilm matrix resulted in an increased permeability for entry of antibiotics, which then cause the eradication of biofilm and inhibit subsequent biofilm formation. Additionally, our results that AuAgNPs inhibited the formation of SDSD biofilms via distinct stress pathways that lead to the downregulation of two genes critical for biofilm production, namely, brpA-like encoding biofilm regulatory protein and fbpA fibronectin-binding protein A. This study provides useful information to assist the development of nanoparticle-based strategies for the active treatment of biofilm-related infections triggered by photoirradiation in the visible.

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“…After the interaction, an aliquot (50 µL) was plated in Mueller Hinton plates and incubated for 16 h at 37 °C. In three independent experiments, duplicated survival bacterial recovery (antibacterial activity, AA) was calculated according to our previous work (España-Sánchez et al, 2018).…”

Section: Antibacterial Activity Of Cunps Against P Aeruginosamentioning

confidence: 99%

Antibacterial behavior and bacterial resistance analysis of P. aeruginosa in contact with copper nanoparticles

et al. 2023

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The present study describes the antibacterial behavior and the bacterial resistance analysis of extremophile Pseudomonas aeruginosa in contact with copper nanoparticles (CuNPs). For this purpose, green synthesis of CuNPs was performed by combined ultrasound-assisted and chemical reduction methods, obtaining semispherical CuNPs ranging from ca. 4-9 nm. Antibacterial activity (AA) of biosynthesized CuNPs demonstrates an antibacterial inhibition of 85 % (LD85) at 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL after 3 h of contact. Bacterial adaptation in contact with CuNPs was observed through the consecutive exposition of microorganisms, presenting a significant increase of LD85 values from 400 μg/mL to 6400 μg/mL after 11 expositions. This behavior demonstrates the bacterial growth adaptation with high-dose of CuNPs. The bacterial resistance mechanism was determined through the overproduction of pyocyanin, associated with oxidative stress events, the genomic polymorphism of resistant bacteria obtained by PCR-RAPDs, and the morphological interaction between P. aeruginosa and CuNPs evidenced by transmission electron microscopy (TEM) micrographs. Our results suggest that under controlled CuNPs exposition, extremophile P. aeruginosa can generate bacterial resistance mechanisms, an important issue for the effective design of antimicrobial nanomaterials.

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Early Stages of Antibacterial Damage of Metallic Nanoparticles by TEM and STEM-HAADF

Cited by 16 publications

References 46 publications

Ag Nanoparticles for Biomedical Applications—Synthesis and Characterization—A Review

Ag Nanoparticles for Biomedical Applications—Synthesis and Characterization—A Review

Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles

Antibacterial behavior and bacterial resistance analysis of P. aeruginosa in contact with copper nanoparticles

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