Early Stage Exposure of 1H,1H,2H,2H-Perfluorooctanesulfonate-Induced Cardiovascular Abnormality in Zebrafish Embryos and Larvae

Abstract

The novel per- and polyfluoroalkyl substances (PFAS) have attracted global attention due to their persistence, bioaccumulation, and various toxicities. Few studies have focused on the toxicological effects of 1H,1H,2H,2H-perfluorooctanesulfonic acid (6:2 FTS), an alternative of perfluorooctanesulfonate (PFOS) detected worldwide. In this study, PFOS was used as a positive control to evaluate the developmental toxicity of 6:2 FTS in zebrafish embryos and larvae. The results of embryo exposure showed that the acute toxicity of 6:2 FTS was lower than that of PFOS. 6:2 FTS could affect the development of zebrafish, including increasing the utilization of yolk sac and the length of the pharyngeal pouch, leading to pericardial edema and inhibiting beating of the heart. Then transgenic zebrafish CZ63 and CZ40 were used to further explore its cardiovascular toxicity. 6:2 FTS could induce vascular hyperplasia, inhibit atrial development, and reduce blood flow velocity in larvae at 72 hours postfertilization. By employing transcriptome analysis and reverse transcription polymerase chain reaction, the involved molecular pathway was finally investigated. Two micromolar 6:2 FTS enhanced the activity of CAT and GSH-Px, involved in calcium signal transduction and myocardial contraction, and affected the MAPK, FoxO, and p53 signaling pathways. Our study can provide scientific insight into the biological safety and health risk of 6:2 FTS.

Integrated Analysis of Per- and Polyfluoroalkyl Substance Exposure and Metabolic Profiling of Elderly Residents Living near Industrial Plants

“…The exposure levels were higher than those in most populations in the non-contamination risk areas; − in comparison to the studies near potential PFAS-impacted communities, the concentrations of these two C8 PFASs were higher than those in the population sampled in 2009, while the PFOA concentration was lower than that in the studies sampled in 2016 and 2018. , As two novel PFASs, the concentrations of 6:2 FTS and 6:2 Cl-PFESA were detected with the mean concentration of 0.04 ng/mL (95% CI: 0.01, 0.07) and 1.36 ng/mL (95% CI: 0.98, 1.74), respectively. 6:2 Cl-PFESA and 6:2 FTS have been used in the metal plating industry and aqueous film-forming foams. , The increased detection frequency and proportion of these two novel PFASs in human sample analysis, including the present study, reflect their increasingly widespread applications.…”

“…6:2 Cl-PFESA and 6:2 FTS have been used in the metal plating industry and aqueous film-forming foams. 36,37 The increased detection frequency and proportion of these two novel PFASs in human sample analysis, including the present study, reflect their increasingly widespread applications.…”

Bimetallic Pd‐Sn Catalytic Electrodes from Deep Eutectic Solvents for Selective Nitrate Reduction Toward Nitrogen

“…[4] Increased nitrate content in water bodies leads to eutrophication, phytoplankton proliferation, sunlight blockage, and toxic gas release. [5] Biological denitrification, [6] Ion exchange, electrodialysis, [7] chemical reduction, and electrocatalysis are among the most common treatments employed for denitrification. Traditional biological treatment have drawbacks like aeration requirements, long operation times, and greenhouse gas emissions.…”