Early spontaneous diploidization of maternal maize haploids generated by in vivo haploid induction

Wu, Penghao; Ren, Jiaojiao; Liang, Li; Chen, Shaojiang

doi:10.1007/s10681-014-1166-5

Cited by 26 publications

(33 citation statements)

References 37 publications

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“…The identification procedure of haploid was as follow: first, primary identification was conducted according to grain color (Nanda and Chase, 1996;Wu et al, 2014): the putative haploids showed purple endosperm and non-purple embryo (Figure 1a), whereas the diploids (Diploid = Doubled haploid) showed purple endosperm and a strongly pigmented purple embryo ( Figure 1b). All putative haploids were seeded in Sanya (N18.14°, E109.31°) examinational station in 2014 winter, and they were further identified according to their growth condition in fields, plant color, and tassel fertility.…”

Section: Induction and Primary Identification Of Haploidmentioning

confidence: 99%

A study on natural recovery of tassel fertilization and doubling method in maize haploids

Jiang¹,

Yang²,

Li³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Doubling method is the technical barriers in maize haploid breeding. It was very important to establish the independent intellectual property rights for doubling method. In this experiment, the maize haploid inducer, TG15, was used for producing maternal haploids. Also, haploids were obtained from two kinds of maternal genotypes involved in the experiment, including high-oil type and common type. Significant differences were observed among offspring of various genotypes in the recovery of haploid fertilization. In 21 hybrid offspring haploids, the average powder rate was 8.28%, and the seed setting rate was 4.98%. The experimental results showed that when the hybrids were treated with 0.08% colchicine, the average powder rate and seed setting rate of offspring haploids were 35.53 and 20.30%, respectively, which were significantly higher than the hybrids with natural recovery ability. This study primarily established the doubling method of haploids called "bud seedling method" in China which was very practicably in maize doubled haploid breeding.

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Section: Induction and Primary Identification Of Haploidmentioning

confidence: 99%

A study on natural recovery of tassel fertilization and doubling method in maize haploids

Jiang¹,

Yang²,

Li³

et al. 2017

Genet. Mol. Res.

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“…Other morphological markers have been used to select haploids such as plant height, plant vigor, and the absence of ligule (Prigge et al, 2012). Moreover, other techniques such as flow cytometry and molecular markers have also been used (Wu et al, 2014;Couto et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Couto

Pinho

et al. 2015

Ciênc. agrotec.

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Artificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities of doubled haploid seeds obtained after the self-fertilization processes were analyzed. It was observed that the germplasm influences haploid induction but not the duplication rates of the tested protocols. Protocol 2 was more efficient for the duplication of haploids, in the percentage of self-fertilized plants after duplication, and in the attainment of doubled haploid lines. Moreover, the haploid inducer line KEMS can produce haploids in a tropical climate. Other markers, in addition to the R1-Navajo system, should be used in the selection of haploid seeds.Index terms: R-Navajo; colchicine; induction of maternal haploids; Zea mays. RESUMOA duplicação cromossômica artificial está dentre as etapas mais importantes na obtenção de duplo-haploides em milho. Este estudo objetivou avaliar a capacidade de indução da linhagem indutora KEMS em clima tropical e testar a eficiência do marcador R1-navajo por meio de citometria de fluxo; avaliar dois protocolos de duplicação cromossômica e, analisar o desenvolvimento dos haploides duplicados no campo. Para isso, quatro genótipos (gerações F1 e F2) foram cruzados com a linhagem KEMS. As sementes obtidas foram selecionadas pelo marcador R1-navajo e submetidas a dois protocolos de duplicação cromossômica. A duplicação foi confirmada por meio de citometria de fluxo. As porcentagens de plantas autofecundadas após duplicação foram analisadas, bem como as quantidades de sementes duplo-haploides obtidas após as autofecundações. Foi observado que o germoplasma influencia a indução de haploides, mas não na taxa de duplicação dos protocolos testados. O protocolo 2 foi mais eficiente na duplicação de haploides, na porcentagem de plantas autofecundadas após duplicação, e na obtenção de linhagens duplo-haploides. Além disso, a linhagem indutora KEMS pode induzir haploides em clima tropical. Outros marcadores além do sistema do R1-navajo devem ser utilizados na seleção de sementes haploides.Termos para indexação: R-navajo; colchicina; indução de haploides maternos; Zea mays.

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“…By inducing the generation of haploid and double haploid chromosome sets, pure lines (doubled haploid (DH) lines) can be obtained in only two generations, thereby, accelerating the progress of variety improvement. There are two traditional methods for obtaining fertile haploids: the spontaneous haploid doubling, for which doubling rates ranging from 0 to 21.4% have been reported in various maize genotypes (Chase, 1952;Barnabas et al, 1999;Han et al, 2009;Jiang et al, 2014) and the artificial chromosome doubling, which can duplicate the chromosome set of haploid plants effectively using doubling agents (Häntzschel and Weber, 2010;Wu et al, 2014). DH lines have been widely used in many fields of breeding and research (Presterl et al, 2007;Wilde et al, 2010;Martin et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Tissue culture characteristics of maize (Zea mays L.) haploid coleoptile sections

Jiang¹,

Jing²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Doubled haploid (DH) technology, which is used for rapidly purifying genetic resources, is a key technology in modern maize breeding. The present study evaluated the tissue culture characteristics of maize haploid coleoptile sections, in order to provide a new way of haploid doubling. With 20 combinations of haploid coleoptile sections, obtained by hybridization within Reid, Tangsipingtou, and Term-tropical groups, as explants, we analyzed the induction and differentiation rate of callus, observed the number of root tip chromosomes in regenerated plants, and analyzed the pollen fertility. In addition, we used 47 SSR markers to analyze the genotypes of regenerated plants. The Reid and Tangsipingtou groups had significantly higher induction rates of haploid coleoptile callus compared to the Term-tropical group. Fifteen haploid plants were obtained which had 10 chromosomes in the root tips as assessed by I-KI staining. It was also noticed that the pollen of pollinated anthers were partially fertile. The haploid plants had genetic stability and showed no variation. The Reid and Tangsipingtou groups had good culture characteristics of haploid coleoptile sections, while the Term-tropical group had poor culture characteristics. Genotypes of haploid plants generated by tissue culture were evidenced to come 16265-16275 (2015) from recombinant types of parents. Thus, this study established a tissue culture system of maize haploid coleoptile.

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Early spontaneous diploidization of maternal maize haploids generated by in vivo haploid induction

Cited by 26 publications

References 37 publications

A study on natural recovery of tassel fertilization and doubling method in maize haploids

A study on natural recovery of tassel fertilization and doubling method in maize haploids

In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Tissue culture characteristics of maize (Zea mays L.) haploid coleoptile sections

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