Early serological diagnosis in visceral leishmaniasis using Crithidia sp. as antigen

López-Brea, M; Iturriaga, R.; Aparicio, Marta

doi:10.1016/0035-9203(79)90105-6

Cited by 3 publications

(2 citation statements)

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“…Por exemplo, características biológicas comuns, como o fato de habitarem em algum momento durante seu ciclo natural de vida um hospedeiro invertebrado (Inverso et al, 1993), pode gerar pressões seletivas comuns ocasionando a existência e manutenção de um repertório molecular semelhante (Santos et al, 2006). Isso poderia também explicar o motivo dos tripanosomatídeos de insetos e plantas servirem como importantes modelos para o entendimento de processos biológicos e bioquímicos dos patogênicos, incluindo estudos mais aplicados que empregam esses organismos no desenvolvimento de novos fármacos (Tasanor et al, 2006) e como antígenos para o imunodiagnóstico (Hedge et al, 1978;Lopez Brea, 1979;Lopes et al, 1981;Monteon et al, 1997;Gonçalves et al, 2002;Griemberg et al, 2006;Martinkovic e Marinculic, 2006).…”

Section: Eletroforese De Proteínas Em Gel De Poliacrilamidaunclassified

“…A proposição alternativa da utilização de antígenos de tripanosmatídeos de insetos para o imunodiagnóstico das leishmanioses e doença de Chagas (Hedge et al, 1978;Lopez Brea, 1979;Lopes et al, 1981) Chagas (Umezawa et al, 1996(Umezawa et al, , 2001) e leishmaniose visceral (Martin et al, 1998;Cibrelus et al, 1999). Da mesma forma, apesar de alguns relatos na literatura, a presença de produtos de excreção-secreção com potencial imunogênico no meio de cultivo de alguns tripanosomatídeos de insetos e plantas é pouco explorada.…”

Section: Eletroforese De Proteínas Em Gel De Poliacrilamidaunclassified

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Caracterização antigênica de moléculas de Leptomonas seymouri reativas com anticorpos anti Leishmania spp e anti T. cruzi

Kesper¹

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Section: Eletroforese De Proteínas Em Gel De Poliacrilamidaunclassified

Caracterização antigênica de moléculas de Leptomonas seymouri reativas com anticorpos anti Leishmania spp e anti T. cruzi

Kesper¹

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Diagnosis and serological follow-up in three patients with visceral leishmaniasis using Crithidia sp. as antigen

López-Brea

1980

Transactions of the Royal Society of Tropical Medicine and Hygi

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Serodiagnosis of Leishmaniasis

Kar

1995

Critical Reviews in Microbiology

152

101

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Leishmaniasis is a spectrum of diseases ranging in severity from cutaneous (CL), post-kala-azar dermal (PKDL), and diffuse cutaneous (DCL) to mucocutaneous (MCL) and visceral (VL) infections that are endemic in 86 tropical and subtropical countries around the world, accounting for 75,000 deaths per year. Different forms of leishmaniases are generally caused by different distinct species of Leishmania having a digenetic life cycle alternating between an aflagellated amastigote form replicative within the macrophages of the host and a flagellated promastigote form that multiplies within the gut of the sandfly. VL, MCL, PKDL, DCL, and CL forms of the disease can be arranged on a priority basis in accordance with the humoral immune responses of host. Generally, the cell-mediated immunity, particularly the delayed-type hypersensitivity to leishmanial antigens, is associated with CL, MCL, PKDL, and cured VL cases. The serodiagnosis of leishmaniasis appears to be an alternative to parasite detection in biopsy samples either by the staining of amastigotes or by culturing the amastigotes, which transform to a promastigote form and replicate. A battery of immunological procedures have been developed or adapted to demonstrate either humoral or cell-mediated immune responses against Leishmania for diagnosis and epidemiological survey. The sensitivity and specificity of such diagnostic methods depend on the type, source, and purity of antigen employed, as some of the leishmanial antigens have common cross-reactive epitopes shared with other microorganisms, particularly Trypanosoma, Mycobacteria, Plasmodia, and Schistosoma. Serodiagnostic techniques for the detection of antileishmanial antibodies have been employed with about 72 to 100, 23 to 90, 83, and 33 to 100% success in VL, CL, MCL, and PKDL patients, respectively. The Leishmanin skin test (LST) is useful to detect MCL and CL, with about 100 and 84% success, respectively. In PKDL, the gradual fall of antileishmanial antibody titer to some extent and the rise of delayed hypersensitivity to the parasite antigen are the characteristic features associated with the chronicity of the disease. The use of whole promastigote as the source of antigens in the direct agglutination test (DAT) and immunofluorescent test (IFAT) gave cross-reactions with the sera of leprosy, tuberculosis, and African trypanosomiasis patients. Again, the use of cell-free extracts of promastigotes generally gave false positive results with the sera of normal human and Chagas' disease, leprosy, tuberculosis, and malaria patients in enzyme-linked immunosorbent assay (ELISA), dot ELISA, immunodiffusion, immunoelectrophoresis, and counter-current immunoelectrophoresis tests.(ABSTRACT TRUNCATED AT 400 WORDS)

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Early serological diagnosis in visceral leishmaniasis using Crithidia sp. as antigen

Cited by 3 publications

References 1 publication

Caracterização antigênica de moléculas de Leptomonas seymouri reativas com anticorpos anti Leishmania spp e anti T. cruzi

Caracterização antigênica de moléculas de Leptomonas seymouri reativas com anticorpos anti Leishmania spp e anti T. cruzi

Diagnosis and serological follow-up in three patients with visceral leishmaniasis using Crithidia sp. as antigen

Serodiagnosis of Leishmaniasis

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