Early-response gene signalling is induced by angiogenic oligosaccharides of hyaluronan in endothelial cells. Inhibition by non-angiogenic, high-molecular-weight hyaluronan

Deed, Richard W.; Rooney, Paul; Kumar, Patricia; Norton, John D.; Smith, Julia; Freemont, Anthony J.; Kumar, Shant

doi:10.1002/(sici)1097-0215(19970410)71:2<251::aid-ijc21>3.0.co;2-j

Cited by 254 publications

(200 citation statements)

References 18 publications

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“…A xenograft study showed that human preadipocyte-seeded HA scaffolds were indeed revascularized with integrated vessels in almost all layers of the sponge with preadipocytes being well distributed over the cross-section of the implanted sponge [19]. Despite the fact that oligomers, the degradation fragments of hyaluronic acid, act on angiogenesis [39] and that preadipocytes have a lower oxygen consumption and higher tolerance for survival than mature fat cells [40] the major disadvantage of cell-based strategies is that the pre-cultured tissue constructs, which must become vascularised once implanted within the recipient, may not be as successful as methods which foster a primary neovascularisation of a biological matrix or scaffold [41]. Embryological adipose tissue formation is preceded by the establishment of a primary vascular plexus, also called the ''primitive fat organ'', and further development of adult adipose tissue is associated with a concomitant increase in the microcirculatory network.…”

Section: Discussionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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Section: Discussionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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“…These findings are consistent with previous observations of an anti-angiogenic effect of high molecular weight hyaluronan but an angiogenic effect of hyaluronan fragments. [45][46][47] Notably, Hyal1 in vitro requires an acidic pH for optimal activity. It is thus possible that close to neutral pH in tissues the secreted or cell surface associated Hyal1 activity 48 may induce only a limit degradation of hyaluronan, giving rise to angiogenic hyaluronan fragments.…”

Section: Discussionmentioning

confidence: 99%

Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors

Jacobson

Rahmanian

Rubin

et al. 2002

Intl Journal of Cancer

112

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Advanced colorectal cancers are often associated with elevated amounts of hyaluronan. To investigate the importance of hyaluronan in colon carcinoma tumor progression, we have expressed by stable transfection hyaluronan synthase 2 (Has2) and hyaluronidase 1 (Hyal1) in the rat colon carcinoma cell line, PROb. We found that hyaluronan overproduction led to a higher growth rate of tumor cells in vitro, and to a faster development of transplantable tumors in syngeneic rats, compared to the mock-transfectants. Has2 transfected PROb cells gave rise to tumors that were significantly less vascularized, but had a significantly larger viable tumor fraction compared to tumors generated from mocktransfectants. In contrast, Hyal1 overexpression suppressed the growth rate of tumor cells both in vitro and in vivo. Moreover, tumors derived from Hyal1-transfected cells had a significantly larger necrotic area than tumors derived from mock-and Has2-transfectants. Our study demonstrates that Has2 overproduction promotes tumorigenicity, whereas Hyal1 overexpression suppresses tumorigenicity in an experimental model for colon carcinoma.

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“…Although originally considered an inert filling material HA is now known to have a number of biological activities including wound healing [1], angiogenesis [2][3] and cancer cell metastasis [4]. In many cases, HA biological function is thought to be related to its molecular size.…”

Section: Introductionmentioning

confidence: 99%

Development of a fluorescent substrate to measure hyaluronidase activity

Zhang

Mummert

2008

Analytical Biochemistry

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A novel fluorescent substrate (termed FRET-HA) to quantitatively assess hyaluronidase activity was developed. Hyaluronan (HA), the major substrate for hyaluronidase, was dual labeled with fluorescein amine and rhodamine B amine. The fluorescein amine fluorescence signal was significantly quenched while the rhodamine B amine signal was significantly enhanced due to fluorescence resonance energy transfer (FRET). In the presence of bovine testes hyaluronidase, cleavage of HA disrupted FRET resulting in a loss of the fluorescein amine quenching that was dependent upon both enzyme concentration and time. Increase in the fluorescein amine signal could be conveniently monitored in both non-continuous and continuous fashions. The K m value for bovine testes hyaluronidase was determined using FRET-HA in a continuous fluorescent assay. Importantly, the estimated K m value for bovine testes hyaluronidase using FRET-HA as the substrate was in excellent agreement with K m values previously reported for this enzyme using native (i.e., unlabeled) HA. Therefore, FRET-HA is a reliable substrate for quantitatively assessing the HA/ hyaluronidase molecular interaction. The simplicity, sensitivity and versatility of the FRET-HA substrate suggest that it will have utility in a variety of assay platforms and should be a new tool for assessing hyaluronidase activity.

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Early-response gene signalling is induced by angiogenic oligosaccharides of hyaluronan in endothelial cells. Inhibition by non-angiogenic, high-molecular-weight hyaluronan

Cited by 254 publications

References 18 publications

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors

Development of a fluorescent substrate to measure hyaluronidase activity

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