Early protein synthesis during germination of barley embryos and its relationship to RNA synthesis

Sopory, Sudhir K.; Puri-Avinashi, M.; Deka, Nijara; Datta, Asis

doi:10.1093/oxfordjournals.pcp.a076039

Cited by 9 publications

(4 citation statements)

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“…Ribosome -inactivating proteins (RIPs) are a group of toxic proteins that can irreversibly inactivate ribosome for protein synthesis [40]. Previous studies suggested that early synthesized proteins were essential for germination and were programmed by a conserved polyadenylate-containing mRNA, preserved in dry embryos [41]. Therefore, it was not surprising that three ribosomal related proteins were identified to be differentially accumulated between hybrid and its parental lines.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Embryos between a Maize Hybrid and Its Parental Lines during Early Stages of Seed Germination

et al. 2013

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In spite of commercial use of heterosis in agriculture, the molecular basis of heterosis is poorly understood. It was observed that maize hybrid Zong3/87-1 exhibited an earlier onset or heterosis in radicle emergence. To get insights into the underlying mechanism of heterosis in radicle emergence, differential proteomic analysis between hybrid and its parental lines was performed. In total, the number of differentially expressed protein spots between hybrid and its parental lines in dry and 24 h imbibed seed embryos were 134 and 191, respectively, among which 47.01% (63/134) and 34.55% (66/191) protein spots displayed nonadditively expressed pattern. Remarkably, 54.55% of nonadditively accumulated proteins in 24 h imbibed seed embryos displayed above or equal to the level of the higher parent patterns. Moreover, 155 differentially expressed protein spots were identified, which were grouped into eight functional classes, including transcription & translation, energy & metabolism, signal transduction, disease & defense, storage protein, transposable element, cell growth & division and unclassified proteins. In addition, one of the upregulated proteins in F1 hybrids was ZmACT2, a homolog of Arabidopsis thaliana ACT7 (AtACT7). Expressing ZmACT2 driven by the AtACT7 promoter partially complemented the low germination phenotype in the Atact7 mutant. These results indicated that hybridization between two parental lines can cause changes in the expression of a variety of proteins, and it is concluded that the altered pattern of gene expression at translational level in the hybrid may be responsible for the observed heterosis.

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Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Embryos between a Maize Hybrid and Its Parental Lines during Early Stages of Seed Germination

et al. 2013

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“…The exact mechanism by which the translation of these mRNAs is prevented till germination is not known. Presence of unprocessed mRNA and lack of some crucial initiation factors (especially eIF-2) are not the reasons, at least in barley embryos, as it has already been established in our laboratory (9,22). In the present study, a cAMP-independent protein kinase has been isolated from barley embryos which is a potent inhibitor of in vitro translation.…”

Section: Discussionmentioning

confidence: 64%

“…The dehydration phase is completed by the 40th d (9). Most synthetic processes, including protein synthesis, would probably be completed by the 30th d. The suppression of translation due to the presence of high level of protein kinase, synthesized during late stages of embryogenesis (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) d), could be the mechanism by which some mRNAs are conserved for use during germination. On germination, the level of protein kinase falls to about 40% by 4 h. As shown in Figure 8, the rate of protein synthesis increased appreciably 4 …”

Section: Discussionmentioning

confidence: 99%