Early protection in sheep against intratypic heterologous challenge with serotype O foot-and-mouth disease virus using high-potency, emergency vaccine

Horsington, Jacquelyn; Zhang, Zhidong; Bittner, Hilary; Hole, Kate; Singanallur, Nagendrakumar Balasubramanian; Alexandersen, Søren; Vosloo, Wilna

doi:10.1016/j.vaccine.2014.11.043

Cited by 24 publications

(27 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine cross–reactivity, the FMDV/O positive sera from sheep vaccinated and challenged with FMDV/O [19] were tested using the FMDV/A cELISA. The results showed no cross–reactivity up to 35 days post challenge (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis

Yang

Bittner

et al. 2016

Virol J

Self Cite

View full text Add to dashboard Cite

BackgroundFoot–and–mouth disease (FMD) is an economically devastating disease that severely limits international trade of animals. Of the seven FMD virus (FMDV) serotypes, serotype A is one of the most widespread cross the world. Currently antibodies to FMDV are detected in animals using the virus neutralization test (VNT) and the enzyme-linked immunosorbent assay (ELISA). The VNT is laborious, time–consuming and reliant on live virus and cell cultures, while ELISA has the advantage of using inactivated antigens and often provides more reproducible results. The aim of this study was to develop a reliable and rapid competitive ELISA (cELISA) for the detection of antibodies to FMDV serotype A (FMDV/A).ResultsA panel of FMDV/A specific monoclonal antibodies (mAbs) was generated and their ability to compete with a polyclonal serum from FMDV/A–infected cattle was examined. Two mAbs inhibited the binding of a polyclonal serum to FMDV/A viruses. The binding epitopes of each were determined as conformational and located on the VP2 viral capsid protein. The FMDV/A cELISA was developed using these two mAbs and FMDV/A inactivated virus as antigen. The diagnostic specificity and sensitivity were 99.7 and 99.3% (98.5–100%) respectively, based on a predetermined cut–off of 50% inhibition. When analysing sera from animals experimentally infected with FMDV/A, the cELISA detected antibodies from 5-days post infection (dpi) and remained positive for at least 21–28 days post infection. Comparison based on the Kappa coefficient showed strong agreement (90–94%) between cELISA and VNT.ConclusionThe cELISA results are comparable to the VNT for antibody detection making it a simple and reliable test to detect antibodies against FMDV/A.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0650-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis

Yang

Bittner

et al. 2016

Virol J

Self Cite

View full text Add to dashboard Cite

show abstract

“…The only other study of experimental inoculation of a similar virus in sheep reported a less virulent phenotype following both coronary band inoculation and contact exposure (Horsington et al, 2015).…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, there were differences in the challenge doses received by the inoculated sheep, and the precise origin and passage history of the virus stocks used were different. Notably, the virus stock used for inoculation by Horsington et al (2015) had been through four passages in tissue culture, whereas the virus used in this current study was derived directly from vesicular epithelium of an infected cow.…”

Section: Discussionmentioning

confidence: 99%

“…Experimental studies characterizing clinical disease, infection dynamics, and pathogenesis of relevant (emerging) FMDV strains in sheep are necessary to facilitate appropriate FMD response measures including evaluation of vaccines for potential emergency use. A recent report demonstrated moderate virulence in sheep of a recently emerged FMDV serotype O from the 2010 outbreak in Republic of Korea (Horsington et al, 2015).…”

mentioning

confidence: 97%

“…Previous studies have confirmed that sheep are susceptible to FMDV infection via nasal instillation (Hughes et al, 2002a;McVicar and Sutmoller, 1971) and exposure to naturally generated aerosols (Aggarwal et al, 2002;Gibson and Donaldson, 1986;Gibson et al, 1984), in addition to coronary band injection and contact exposure Burrows, 1968;Horsington et al, 2015). However, the use of different challenge viruses at varying doses generally precludes detailed comparisons of experimental systems based on these works.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Clinical and virological dynamics of a serotype O 2010 South East Asia lineage foot-and-mouth disease virus in sheep using natural and simulated natural inoculation and exposure systems

Stenfeldt

Pacheco

Singanallur

et al. 2015

Veterinary Microbiology

Self Cite

View full text Add to dashboard Cite

Within-host infection dynamics of a recent field isolate of foot-and-mouth disease virus (FMDV), serotype O, topotype South East Asia, lineage Myamar'98 were evaluated in sheep using four different systems for virus exposure. Two novel, simulated natural, inoculation systems consisting of intra-nasopharyngeal (INP) deposition and aerosol inoculation were evaluated in comparison with two conventional systems: coronary band inoculation and direct contact exposure. All four exposure systems were efficient in generating consistently severe, generalized FMD with synchronous clinical characteristics within exposure groups, indicating that this Myanmar98 strain is highly virulent in sheep. Clinical and virological dynamics were similarly rapid following INP- and coronary band inoculation, with both systems leading to significantly earlier detection of virus shedding when compared to aerosol inoculation and contact exposure. The data presented herein support application of the two optimized simulated natural inoculation systems as valid alternatives to conventionally used exposure systems for studies of FMDV pathogenesis and vaccinology in sheep. Furthermore, the data suggest that targeted exposure of the ovine pharynx is highly efficient for generating consistent FMDV infection, which supports critical involvement of this anatomic region as a site of primary virus replication in sheep.

show abstract

Efficacy of a high potency O1 Manisa monovalent vaccine against heterologous challenge with foot-and-mouth disease virus of O/SEA/Mya-98 lineage in sheep

et al. 2017

View full text Add to dashboard Cite

Potency tests for commercial oil-adjuvanted foot-and-mouth disease (FMD) vaccines are usually carried out in cattle, using a full dose (2 ml) of vaccine and homologous virus challenge. However, in sheep the recommended vaccine dose is half of the cattle dose (1 ml) and most vaccines have not been potency tested for this species, especially with heterologous viruses. To determine the efficacy of a high potency (>6PD) FMD virus (FMDV) O1Manisa vaccine in sheep, we carried out a study using a heterologous FMDV (FMDV O/SKR/2010 - Mya-98 strain) challenge. Groups of seven animals each were vaccinated with 2×, 1×, 1/2× or 1/4× dose (2 ml, 1 ml, 0.5 ml or 0.25 ml respectively) and challenged at 7 days post vaccination (dpv). Only 3 of the 7 sheep in the group vaccinated with 2 ml were protected. With 2 additional groups, receiving double or single doses and challenged at 14 dpv, 4 of 7 sheep were protected in each group. None of the sheep had measurable neutralising antibodies against the vaccine or challenge virus at 7 dpv. However, all vaccinated animals challenged at 14 dpv had a homologous neutralising response against FMDV O1 Manisa on the day of challenge and all but one animal also had a heterologous response to FMDV O/SKR/2010. Infectious FMDV and viral RNA could be found in nasal swabs between 1 and 6 days post challenge (dpc) in most vaccinated sheep, but those vaccinated with higher doses or challenged at 14 dpv showed significant decreases in the level of FMDV detection. Intermittent virus shedding was noticed between 1 and 35 dpc in all vaccinated groups, but persistent infection could be demonstrated only in 4 sheep (20%). This study showed that at the recommended dose, a high potency (>6 PD) FMDV O1Manisa vaccine does not protect sheep against a heterologous challenge at 7 dpv. However, partial protection was observed when a double dose was used at 7 dpv or when double or single dose vaccinated sheep were challenged at 14 dpv.

show abstract

Early protection in sheep against intratypic heterologous challenge with serotype O foot-and-mouth disease virus using high-potency, emergency vaccine

Cited by 24 publications

References 34 publications

Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis

Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis

Clinical and virological dynamics of a serotype O 2010 South East Asia lineage foot-and-mouth disease virus in sheep using natural and simulated natural inoculation and exposure systems

Efficacy of a high potency O1 Manisa monovalent vaccine against heterologous challenge with foot-and-mouth disease virus of O/SEA/Mya-98 lineage in sheep

Contact Info

Product

Resources

About