Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples

González, F.; Sulon, Joseph; Garbayo, J. M.; Batista, M.; Cabrera, Fernando; Calero, P.; Gracia, A.; Beckers, J.F.

doi:10.1016/s0093-691x(99)00165-x

Cited by 54 publications

(28 citation statements)

References 24 publications

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“…Interestingly, a rapid increase in PAG concentration during pregnancy is characteristic of ovine [43] and caprine species [44]. However, it differs largely from those described in bovine species, in which PAG concentrations increase slowly during the first trimester of gestation [39,45].…”

Section: Discussionmentioning

confidence: 99%

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

et al. 2013

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BackgroundPregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species.ResultsAmino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females.ConclusionsOur results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.

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Section: Discussionmentioning

confidence: 99%

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

et al. 2013

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“…Although bPAG and cPAG have been successfully used for detecting pregnancy in cattle (Zoli et al 1992;Szenci et al 1998) and goats, respectively, (Folch et al 1993, Gonzalez et al 1999, there is no data evaluating the accuracy of oPAG assays for diagnosing pregnancy in sheep.…”

Section: Ovine Pregnancy-associated Glycoproteins (Opags)mentioning

confidence: 99%

Pregnancy Dagnosis in Sheep: Review of the Most Practical Methods

Karen¹,

Kovács²,

Beckers³

et al. 2001

Acta Vet. Brno

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Various practical methods have been used for pregnancy diagnosis in sheep. Both pregnancy and fetal numbers are accurately diagnosed by using radiography after day 70 of gestation. Rectal abdominal technique detects pregnancy with an accuracy of 66 to 100% from d 49 to 109 of gestation, however, it has a low (17 to 57%) accuracy for determining multiple fetuses. Progesterone assays have a high sensitivity (88% to 100%) and a low specificity (60% to 72%) at d 16 to 18. Estrone sulphate assay accurately detects pregnant ewe at d 30 to 35. Ovine pregnancy specific protein B (PSPB) assay accurately (100%) detects pregnancy from d 26 after breeding onwards. The accuracy of progesterone, estrone sulphate and oPSPB assays for determining fetal numbers is relatively low. A-mode and Doppler ultrasonic techniques accurately detect pregnancy during the second half of gestation. Fetal numbers cannot be determined by A-mode ultrasound, while the Doppler technique needs experience to achieve high accuracy. Transrectal B-mode, real time ultrasonography identifies the embryonic vesicles as early as d 12.8 after mating, but the sensitivity of the technique for pregnancy is very low (12%) earlier than 25 d after mating. Transabdominal B-mode ultrasonography achieved high accuracy for pregnancy diagnosis (94% to 100%) and the determination of fetal numbers (92% to 99%) on d 29 to 106 of gestation. Realtime, B-mode ultrasonography appears to be the most practical and accurate method for diagnosing pregnancy and determining fetal numbers in sheep.

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“…During pregnancy, the plasma ovPAG concentrations vary according to the breed of the ewe, the stage of pregnancy, and the number and genotype of the fetuses [13,14]. Moreover, the antiserum used in the radioimmunoassay of PAG is a source of variation for PAG concentrations in the same animal [15,16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of false transrectal ultrasonographic pregnancy diagnoses in sheep by measuring the plasma level of pregnancy-associated glycoproteins

Karen

Beckers²,

Sulon³

et al. 2003

Reprod. Nutr. Dev.

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-The present study was undertaken to investigate to what extent pregnancy diagnoses made by transrectal ultrasonography could be confirmed by measurements of plasma concentration of ovine pregnancy-associated glycoproteins (ovPAG). A total of 424 Awassi × Merino ewes were synchronized for estrus and examined by transrectal ultrasonography. In Experiment 1, the ewes (n = 156) were repeatedly scanned in a standing position on d 29, 36 and 50 of gestation. Similarly, the ewes (n = 268) in Experiment 2 were scanned on d 24, 29 and 34 of gestation, but these ewes were fasted for 12 h prior to the examination and the abdominal wall of each animal was lifted up by the hands of the assistant during the scanning. Blood samples were withdrawn after each transrectal ultrasonographic examination in both experiments. Ovine PAG concentrations were measured in plasma by a heterologous radioimmunoassay and the cut-off value for pregnancy was ≥ 1 ng·mL -1 . Based on the lambing performance, in Experiment 1, altogether 47 false negative and 38 false positive diagnoses were made by transrectal ultrasonography in 24 and 33 ewes, respectively between d 29 and 50 of gestation. In Experiment 2, altogether 8 false negative and 13 false positive diagnoses both were made in 7 ewes between d 24 and 34 of gestation. In both experiments, all ewes with false negative diagnoses had ovPAG concentrations higher than the threshold level for pregnancy diagnosis and all ewes with false positive diagnoses had ovPAG concentrations lower than the threshold of pregnancy. Furthermore, by the PAG-RIA test all lambed or aborted ewes (n = 63) were correctly diagnosed as pregnant and with three exceptions, all non-lambed ewes (n = 361) were correctly diagnosed as non-pregnant during the examined periods of both experiments.transrectal ultrasonography / ovPAG / pregnancy diagnosis / sheep Reprod. Nutr. Dev. 43 (2003) 577-586 577

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Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples

Cited by 54 publications

References 24 publications

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Pregnancy Dagnosis in Sheep: Review of the Most Practical Methods

Evaluation of false transrectal ultrasonographic pregnancy diagnoses in sheep by measuring the plasma level of pregnancy-associated glycoproteins

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