Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis

Li, Hongchang; Fan, Xin-Juan; Chen, Yafeng; Tu, Jia-min; Pan, Li-yun; Chen, Teng; Yin, Peihao; Peng, Wen; Feng, Dan

doi:10.1016/j.pan.2015.12.177

Cited by 20 publications

(9 citation statements)

References 34 publications

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“…However, there was no significant difference between the HMA mice fed with the goat, mare, and human milk on the level of serum sIgA. It is also known that serum DAO, d -lactic acid, and endotoxin levels indicate early intestinal damages . Our results demonstrated that cow and goat milk significantly altered the intestinal mucosal barrier; however, serum d -lactic acid amounts in mice fed with the mare milk were not significantly different from the mice fed with human milk.…”

Section: Discussioncontrasting

confidence: 55%

Cow, Goat, and Mare Milk Diets Differentially Modulated the Immune System and Gut Microbiota of Mice Colonized by Healthy Infant Feces

Xie²,

Chen

et al. 2020

J. Agric. Food Chem.

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Studies on the possible alternative supplements to breastmilk are gaining research interests. Although milk from cow, goat, and mare is nutritious, its effects on the relationship between the immune system, metabolites, and gut microbiota remain unclear. This study aimed to comprehensively evaluate the effects of cow, goat, and mare milk on the immune system, metabolites, and gut microbiota of mice colonized by healthy infant feces using human milk as a standard. We examined the serum biochemistry parameters, immunity indicators, T cells, gut microbiota abundance, and metabolites. Results showed that the impact of human milk on alanine transaminase, glutamic oxaloacetic transaminase, total protein, globulin, and glucose values was different from the cow, goat, and mare milk types. The effects of mare milk on the percentage of CD4 + T, Th1, Th2, Th17, and Treg cells, and the levels of IL-2, IL-4, sIgA, and D-lactic acid in the serum of the human microbiota-associated mice were comparable to those of human milk. Also, bacterial 16S rRNA gene sequence analysis revealed that human milk enriched the relative abundance of Akkermansia and Bacteroides, cow milk increased the relative abundance of Lactobacillus, goat milk increased the relative abundance of Escherichia-Shigella, and mare milk improved the relative abundance of Klebsiella. Besides, mare milk was similar to human milk in the concentration of the metabolites we analyzed. Our findings suggest that mare milk can positively modulate the gut microbiota and immunity status of infants and thus could be a possible replacement for human milk.

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Section: Discussioncontrasting

confidence: 55%

Cow, Goat, and Mare Milk Diets Differentially Modulated the Immune System and Gut Microbiota of Mice Colonized by Healthy Infant Feces

Xie²,

Chen

et al. 2020

J. Agric. Food Chem.

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“…The normal intestinal mucosal barrier maintains a dynamic balance and effectively prevents intestinal bacteria and ET translocation ( Garside et al, 2004 ). The impairment of intestinal immunity and barrier function may lead to the increase of the permeability of the intestinal mucosa barrier to affect intestinal function for effectively preventing bacteria and their products such as ET into the lymphatic vessels of intestines and blood flow, further promoting the release of inflammatory mediators to cause systemic inflammation ( Li et al, 2016 ; Vindigni et al, 2016 ). In the present studies, we showed that STC induced SAP produced severely pathological injury in the intestinal mucosas and tissues in the rats ( Figure 1A ).…”

Section: Discussionmentioning

confidence: 99%

Free Total Rhubarb Anthraquinones Protect Intestinal Injury via Regulation of the Intestinal Immune Response in a Rat Model of Severe Acute Pancreatitis

et al. 2018

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Intestinal mucosal immune barrier dysfunction plays a key role in the pathogenesis of severe acute pancreatitis (SAP). Rhubarb is a commonly used traditional Chinese medicine as a laxative in China. It markedly protects pancreatic acinar cells from trypsin-induced injury in rats. Free total rhubarb anthraquinones (FTRAs) isolated and extracted from rhubarb display the beneficial effects of antibacteria, anti-inflammation, antivirus, and anticancer. The principal aim of the present study was to investigate the effects of FTRAs on the protection of intestinal injury and modification of the intestinal barrier function through regulation of intestinal immune function in rats with SAP. We established a rat model of SAP by injecting 3.5% sodium taurocholate (STC, 350 mg/kg) into the biliopancreatic duct via retrograde injection and treated the rats with FTRAs (36 or 72 mg/kg) or normal saline (control) immediately and 12 h after STC injection. Then, we evaluated the protective effect of FTRAs on intestinal injury by pathological analysis and determined the levels of endotoxin (ET), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), nitric oxide (NO), myeloperoxidase (MPO), capillary permeability, nucleotide-binding oligomerization domain-like receptors 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD domain (ASC), casepase-1, secretary immunoglobulin A (SIgA), regulatory T cells (Tregs), and the ratio of Th1/Th2 in the blood and/or small intestinal tissues or mesenteric lymph node (MLN) cells. Moreover, the chemical profile of FTRAs was analyzed by HPLC-UV chromatogram. The results showed that FTRAs significantly protected intestinal damage and decreased the levels of ET, IL-1β, TNF-α, and NO in the blood and TNF-α, IL-1β, and protein extravasation in the intestinal tissues in SAP rats. Furthermore, FTRAs significantly decreased the expressions of NLRP3, ASC, and caspase-1, the number of Tregs and the ratio of Th1/Th2, while significantly increased the expression of SIgA in the intestinal tissues and/or MLN cells in SAP rats. Our results indicate that FTRAs could protect intestinal injury and improve intestinal mucosal barrier function through regulating immune function of SAP rats. Therefore, FTRAs may have the potential to be developed as the novel agent for the treatment of SAP clinically.

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“…[ 1 ] The high mortality is due to the serious complications including systemic inflammatory response syndrome (SIRS) and multiple organ failure (MOF). [ 2 ] Animal and human studies [ 3 , 4 ] have demonstrated that the damage of intestinal barrier function accelerates the development of local and systemic infectious complications. In the early stage of SAP, the intestinal permeability has been significantly increased, which results in the translocation of inflammatory mediators and toxic products.…”

Section: Introductionmentioning

confidence: 99%

Enteral nutrition provided within 48 hours after admission in severe acute pancreatitis

Song

Zhong

et al. 2018

Medicine

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Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis

Cited by 20 publications

References 34 publications

Cow, Goat, and Mare Milk Diets Differentially Modulated the Immune System and Gut Microbiota of Mice Colonized by Healthy Infant Feces

Cow, Goat, and Mare Milk Diets Differentially Modulated the Immune System and Gut Microbiota of Mice Colonized by Healthy Infant Feces

Free Total Rhubarb Anthraquinones Protect Intestinal Injury via Regulation of the Intestinal Immune Response in a Rat Model of Severe Acute Pancreatitis

Enteral nutrition provided within 48 hours after admission in severe acute pancreatitis

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