Early postnatal stress affects 5-HT1A receptor function in the medial prefrontal cortex in adult rats

Nishimura

2015

Eur J of Neuroscience

Early-life stress increases the prevalence of psychiatric diseases associated with emotional dysregulation. Emotional regulation requires the inhibitory influence of the medial prefrontal cortex (mPFC) on amygdalar activity, and dysfunction of this system is believed to induce anxiety. Because mPFC and amygdala have dense reciprocal connections and projections between them continue to develop until adolescence, early-life stress may impair the function of this circuit and cause emotional dysregulation. We examined the effects of stress during circuit development on anxiety-like behaviors, neural activities in the mPFC and amygdala, and impulse transmission in the mPFC-amygdala circuit in adult rats. Early-life stress, unpredictable stress twice a day for 12 days following early weaning, increased anxiety-like behaviors in the open-field and elevated plus-maze tests. In the open-field test, stress altered Fos expression in the mPFC and amygdala. Compared to non-stressed rats, which were exposed to neither unpredictable stress nor early weaning, stressed rats exhibited decreased Fos expression in the right superficial layers of the infralimbic cortex and increased Fos expression in the right basolateral amygdala and both sides of the central amygdala. Electrophysiological analysis revealed that excitatory latencies of mPFC neurons to amygdalar stimulation in stressed rats were significantly longer than control rats in the right, but not left, hemisphere. Stress had no effect on excitatory latencies of amygdalar neurons to mPFC stimulation in the mPFC-amygdala circuits in the both hemisphere. These data suggest that early-life stress impairs the mPFC-amygdala circuit development, resulting in imbalanced mPFC and amygdala activities and anxiety-like behaviors.

Section: Effects Of Early-life Stress On Adult Anxiety Behaviorsmentioning

confidence: 99%

Early‐life stress induces anxiety‐like behaviors and activity imbalances in the medial prefrontal cortex and amygdala in adult rats

Nishimura

2015

Eur J of Neuroscience

“…We have also recently reported that pups exposed to footshock (FS) stress from postnatal days 21 to 25 (3wFS) have an altered anxiety-related behavior as adult rats (Konno et al 2007;Matsumoto et al 2008;Koseki et al 2009;Matsuzaki et al 2009). In these studies, the 3wFS increased the ratio of spent time in open arms in an elevated plus-maze test and reduced the number of 5-HT-like immunoreactive cells in the median raphe nucleus (MRN) compared with the non-FS control group.…”

Section: Introductionmentioning

confidence: 95%

Juvenile stress attenuates the dorsal hippocampal postsynaptic 5-HT1A receptor function in adult rats

et al. 2010

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Neurourology and Urodynamics

“…Citalopram hydrobromide, Selective Serotonin Reuptake Inhibitors (SSRI), and (± )‐8‐hydroxy‐2‐dipropylaminotetralin (8‐OH‐DPAT), a 5‐HT1A agonist, were dissolved in ACSF, and directly perfused into the PFC through the dialysis probe. The doses of drugs were chosen based on preliminary results and previous reports …”

Section: Methodsmentioning

confidence: 99%

“…Following the administration of citalopram, 8‐OH‐DPAT (300 µM) was administered into the PFC via reverse microdialysis for 180 min. Previous studies revealed that 8‐OH‐DPAT decreased 5‐HT levels in the PFC following the administration of citalopram . Physiological saline was infused into the bladder continuously from 0 min to 360 min.…”

Section: Methodsmentioning

confidence: 99%

The role of serotonergic mechanism in the rat prefrontal cortex for controlling the micturition reflex: An in vivo microdialysis study

Chiba

Mitsui

Kitta

et al. 2015

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