Early Postnatal Food Intake Alters Myofiber Maturation in Pig Skeletal Muscle

Lefaucheur, Louis; Ecolan, Patrick; Barzic, Yves-Marie; Marion, Julia; Dividich, Jean Le

doi:10.1093/jn/133.1.140

Cited by 50 publications

(46 citation statements)

References 48 publications

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“…In-Gel immunodetection (data not shown) with the MF-20 antibody that recognizes all MHC isoforms confirmed that the bands observed by silver staining after gel electrophoresis were all isoforms of MHC. Consistent with the findings of Lefaucheur et al (2001), the isoform that displayed the greatest electrophoretic mobility was identified as MHCIb. Using immunohistochemistry, positive reactivity was also observed using BAD-5 (specific staining for MHCIb) for both adult triceps brachii tissue and fetal day 90 semitendinosus tissue, and NOQ7.5.4D also showed specific staining for MHCIb in day 90 semitendinosus tissue ( Fig.…”

Section: Discussionsupporting

confidence: 80%

“…8). As reported by Lefaucheur et al (2001) using newborn piglet tissue, none of the primary fibres reacted with the antibody F88.12F8 (specific to MHCIa) in day 90 semitendinosus tissue, confirming that MHCIa is not present in the semitendinosus muscle until after birth in the pig.…”

Section: Discussionsupporting

confidence: 80%

“…In contrast to Lefaucheur et al (2001) who reported that all three adult MHCII isoforms (i.e. IIa, IIx and IIb) co-migrated using standard SDS-PAGE techniques, three fast MHC isoforms were observed in adult tissue in the present study and a total of four bands in these adult tissue samples is in agreement with the immunohistochemical evidence for the Ib, IIa, IIb and IIx isoforms.…”

Section: Discussionsupporting

confidence: 79%

“…Positive bands were not observed using the developmental antibodies NCL-d, NCL-n and BF-45 due to the apparent lack of reactivity of these antibodies with porcine muscle. However, the two upper bands observed in the day 90 samples were tentatively identified as fetal (band b) and embryonic (band a), based on the gel patterns established by Lefaucheur et al (2001). As expected, these fetal and embryonic bands were not observed in adult tissue.…”

Section: In-gel Immunodetectionsupporting

confidence: 55%

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Number of conceptuses in utero affects porcine fetal muscle development

Town¹,

Putman²,

Turchinsky³

et al. 2004

Reproduction

123

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Unmodified, third parity, control sows (CTR; n 5 30) or sows subjected to unilateral oviduct ligation before breeding (LIG; n 5 30), were slaughtered at either day 30 or day 90 of gestation and used to determine the effects of numbers of conceptuses in utero on prenatal, and particularly muscle fibre, development. Ovulation rate, number of conceptuses in utero, placental and fetal size, and (day 90 sows) fetal organ and semitendinosus muscle development were recorded. Tubal ligation reduced (P < 0.05) the number of viable embryos at day 30 and fetuses at day 90. Placental weight at day 30 and day 90, and fetal weight at day 90, were lower (P < 0.05) in CTR sows. All body organs except the brain were lighter, and the brain:liver weight ratio was higher in CTR fetuses (P < 0.05), indicative of brain sparing and intrauterine growth restriction in fetuses from CTR sows. Muscle weight, muscle cross-sectional area and the total number of secondary fibres were also lower (P < 0.05) in CTR fetuses. The number of primary fibres, the secondary:primary muscle fibre ratio, and the distribution of myosin heavy chainIb, -IIa, fetal and embryonic isoforms did not differ between groups. Thus, even the relatively modest uterine crowding occurring naturally in CTR sows negatively affected placental and fetal development and the number of secondary muscle fibres. Consequences of more extreme crowding in utero on fetal and postnatal development, resulting from changing patterns of early embryonic survival, merit further investigation.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 79%

Section: In-gel Immunodetectionsupporting

confidence: 55%

See 2 more Smart Citations

Number of conceptuses in utero affects porcine fetal muscle development

Town¹,

Putman²,

Turchinsky³

et al. 2004

Reproduction

123

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show abstract

“…In pigs, undernutrition (feed intake restriction) increases the proportion of type I fibres and decreases the proportion of type II fibres in the longissimus muscle (Lefaucheur et al, 2003). Further, sows finished out-of-doors had more type I and IIA fibres and smaller fibres than indoor-fattened pigs (Abreu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

Men

Deng

et al. 2013

Animal

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The objective of this study is to investigate the age-related changes of and the effects of dietary conjugated linoleic acid (CLA) on muscle-fibre types in commercial pigs. We divided 25 crossbred male pigs into five age groups (7, 30, 60, 100 and 180 days) and 30 finishing pigs into two dietary groups (one fed a CLA-enriched diet and the other fed a control diet for 30 days). We analysed the composition (%) of myosin heavy-chain (MyHC) mRNA according to the absolute copies of each MyHC (I, IIa, IIb and IIx) mRNA, and the activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in the longissimus muscle. From days 7 to 180, the MyHC I mRNA abundance and SDH and MDH activities presented a decreasing trend, the MyHC IIb mRNA abundance presented a steady trend and the MyHC IIa and IIx mRNA abundances presented an increasing trend. On day 30, MyHC I and IIb mRNA abundances were at their lowest (P , 0.05), and the MyHC IIa and IIx mRNA abundances were at their highest (P , 0.05). In the CLA group, the MyHC I mRNA abundance and the activities of SDH and MDH were improved in the longissimus muscle, whereas pressure loss, drip loss and average back fat depth significantly decreased (P , 0.01) and shear force significantly increased (P , 0.01). Loin eye area, feed conversion rate and meat colour showed some tendency to be improved. These results indicated that more oxidative fibres might convert to glycolytic fibres with increasing age or weight, and that the early developmental stage might be a key stage for this conversion. During the finishing stage, the proportion of oxidative fibres might be increased by dietary CLA supplementation, which may contribute to the water-holding capacity of meat. The results would provide an important basis for the application of muscle-fibre types in the improvement of pork quality.

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Alterations in the gastrocnemius muscle of undernourished suckling rats

Silvado

Werneck

2006

Muscle and Nerve

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Protein-energy undernutrition (PEU) during both the gestational and suckling periods causes a reduction in muscle fiber cross-section and a delay in fiber type differentiation. Such changes are not found when PEU occurs after the suckling period. To elucidate the consequences of PEU on muscle during the suckling period, we undernourished (UND) newborn rats by reducing their food intake, including a 6-hour fast every day, and doubling the number of pups suckled by the nursing rat. At day 24 of life, the gastrocnemius muscle showed a discrete reduction in lipids in the fibers of 5 control rats, and in 15 UND rats (P < 0.001). The type 2a and 2b fibers of the UND rats showed a smaller cross-sectional area than control rats (P < 0.05). The distribution frequency histogram for the muscle fibers was skewed to the left in the undernourished rats, and this was significant for type 2a and 2b fibers. PEU during the suckling period may thus induce a delay in muscle fiber maturation but does not produce significant structural alterations.

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Early Postnatal Food Intake Alters Myofiber Maturation in Pig Skeletal Muscle

Cited by 50 publications

References 48 publications

Number of conceptuses in utero affects porcine fetal muscle development

Number of conceptuses in utero affects porcine fetal muscle development

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

Alterations in the gastrocnemius muscle of undernourished suckling rats

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