Early plant growth and biochemical responses induced by Azospirillum brasilense Sp245 lipopolysaccharides in wheat (Triticum aestivum L.) seedlings are attenuated by procyanidin B2

Vallejo-Ochoa, Juan; López-Marmolejo, Mariel; Hernández-Esquivel, Alma Alejandra; Méndez-Gómez, Manuel; Suárez-Soria, Laura Nicolasa; Castro-Mercado, Elda; García-Pineda, Ernesto

doi:10.1007/s00709-017-1180-2

Cited by 13 publications

(3 citation statements)

References 62 publications

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“…The plant-growth-promotion effect of LPS has been reported so far on wheat (Triticum aestivum) treated with Azospirillum brasilense Sp245 LPS, but its effective concentration was 100 mg mL −1 [24]. LPS from PNSB in the present study was effective at 100 pg mL −1 , indicating that the LPS of PNSB had extremely high plant-growth-promoting activity.…”

Section: Discussioncontrasting

confidence: 52%

Plant-Growth-Promoting Effect by Cell Components of Purple Non-Sulfur Photosynthetic Bacteria

et al. 2022

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Rhodobacter sphaeroides, a purple non-sulfur photosynthetic bacterium (PNSB), was disrupted by sonication and fractionated by centrifugation into the supernatant and pellet. The effects of the supernatant and pellet on plant growth were examined using Brassica rapa var. perviridis (komatsuna) in the pot experiments. Both fractions showed growth-promoting effects: the supernatant at high concentrations (1 × 107 to 4 × 107 cfu-equivalent mL−1) and the pellet at a low concentration of 2 × 103 cfu-equivalent mL−1). We expected lipopolysaccharide (LPS) to be the active principle of the pellet fraction and examined the effects of LPS on the growth of B. rapa var. perviridis. The growth of the plants was significantly enhanced by the foliar feeding of R. sphaeroides LPS at concentrations ranging from 10 to 100 pg mL−1. The present study is the first report indicating that LPS acts as one of the active principles of the plant-growth-promoting effect of PNSB.

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Section: Discussioncontrasting

confidence: 52%

Plant-Growth-Promoting Effect by Cell Components of Purple Non-Sulfur Photosynthetic Bacteria

et al. 2022

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“…Chávez-Herrera et al [ 28 ] examined the effects of LPS from Azospirillum brasilense Sp245 on the leaf and root development of Triticum aestivum by pot experiment, and found that both leaf and root development were promoted by LPS. The same research group reported the ROS (H 2 O 2 ) generation and plant growth promotion (leaf and root) by LPS from A. brasilense Sp245 in T. aestivum [ 85 ]. They also treated the LPS-elicited plants with catalase, which catalyzes the decomposition of hydrogen peroxide to water and oxygen, and found a decrease in H 2 O 2 content in the roots and also a decrease in the growth of seedlings.…”

Section: Discussionmentioning

confidence: 99%

Effects of LPS from Rhodobacter sphaeroides, a Purple Non-Sulfur Bacterium (PNSB), on the Gene Expression of Rice Root

et al. 2023

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The effects of lipopolysaccharide (LPS) from Rhodobacter sphaeroides, a purple non-sulfur bacterium (PNSB), on the gene expression of the root of rice (Oryza sativa) were investigated by next generation sequencing (NGS) RNA-seq analysis. The rice seeds were germinated on agar plates containing 10 pg/mL of LPS from Rhodobacter sphaeroides NBRC 12203 (type culture). Three days after germination, RNA samples were extracted from the roots and analyzed by RNA-seq. The effects of dead (killed) PNSB cells of R. sphaeroides NBRC 12203T at the concentration of 101 cfu/mL (ca. 50 pg cell dry weight/mL) were also examined. Clean reads of NGS were mapped to rice genome (number of transcript ID: 44785), and differentially expressed genes were analyzed by DEGs. As a result of DEG analysis, 300 and 128 genes, and 86 and 8 genes were significantly up- and down-regulated by LPS and dead cells of PNSB, respectively. The plot of logFC (fold change) values of the up-regulated genes of LPS and PNSB dead cells showed a significant positive relationship (r2 = 0.6333, p < 0.0001), indicating that most of the effects of dead cell were attributed to those of LPS. Many genes related to tolerance against biotic (fungal and bacterial pathogens) and abiotic (cold, drought, and high salinity) stresses were up-regulated, and the most strikingly up-regulated genes were those involved in the jasmonate signaling pathway, and the genes of chalcone synthase isozymes, indicating that PNSB induced defense response against biotic and abiotic stresses via the jasmonate signaling pathway, despite the non-pathogenicity of PNSB.

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“…A. brasilense LPS are highly active in interactions with plant roots (Fedonenko et al 2001;Matora et al 1995) and in induction of plant responses (Evseeva et al 2011). Furthermore, A. brasilense Sp245 LPS can promote wheat plant growth in vitro (Vallejo-Ochoa et al 2018) and under greenhouse conditions (Chavez-Herrera et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of Azospirillum brasilense Sp245 and Pseudomonas aeruginosa PAO1 Lipopolysaccharides on Wheat Seedling Growth and Peroxidase Activity

Hernández-Esquivel

Castro-Mercado

García-Pineda

2020

J Plant Growth Regul

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The effects of lipopolysaccharides (LPS) from Azospirillum brasilense Sp245, a plant growth-promoting rhizobacteria, and Pseudomonas aeruginosa PAO1, a pathogenic bacterium, on plant growth and peroxidase (POD) activity were assessed on wheat seedlings. A. brasilense LPS (100 µg/mL) increased total length, and total fresh weight in wheat seedlings 4 days after treatment. P. aeruginosa LPS did not show effect on plant growth. A. brasilense LPS increased root hairs length similar to whole cells, while P. aeruginosa LPS increased root hairs density and slightly root hairs length. Both LPS increased POD activity and hydrogen peroxide (H 2 O 2 ) content in root; however, the LPS from the pathogenic bacterium generated higher increments. The peroxidase inhibitor salicylhydroxamic acid (SHAM) inhibited plant growth, which was not recovered by the addition of LPS neither A. brasilense nor P. aeruginosa. POD activity stimulated by LPS was calcium-dependent as confirmed by the addition of the calcium channel blocker LaCl 3 . The results suggest that plant cells sense differentially LPS from beneficial or pathogenic bacteria and that calcium is needed to respond to the presence of both LPS.

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Early plant growth and biochemical responses induced by Azospirillum brasilense Sp245 lipopolysaccharides in wheat (Triticum aestivum L.) seedlings are attenuated by procyanidin B2

Cited by 13 publications

References 62 publications

Plant-Growth-Promoting Effect by Cell Components of Purple Non-Sulfur Photosynthetic Bacteria

Plant-Growth-Promoting Effect by Cell Components of Purple Non-Sulfur Photosynthetic Bacteria

Effects of LPS from Rhodobacter sphaeroides, a Purple Non-Sulfur Bacterium (PNSB), on the Gene Expression of Rice Root

Comparative Effects of Azospirillum brasilense Sp245 and Pseudomonas aeruginosa PAO1 Lipopolysaccharides on Wheat Seedling Growth and Peroxidase Activity

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