Early phenotypical diagnoses in Trembler-J mice model

Rosso, Gonzalo; Cal, Karina; Canclini, Lucía; Damián, Juan Pablo; Ruiz, Paul; Rodríguez, Héctor; Sotelo, Julio; Vazquez, Cristina; Kun, Alejandra

doi:10.1016/j.jneumeth.2010.04.010

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…The main changes affecting the SNP, begins to be evident from post-natal day 20 (P20), showing a characteristic body tremor and the impossibility of abduction and extension of the hind legs (from P11), as we recently described (Rosso et al, 2010). Typically the mutation is confirmed by individual genotyping of mice (mainly by PCR-RFLP; Notterpek et al, 1997;Fortun et al, 2005;Khajavi et al, 2007, Rosso et al, 2010.…”

Section: Schwann Cells' Ribosomes Are Involved In Axonal Sprouting Ofmentioning

confidence: 90%

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The Schwann Cell-Axon Link in Normal Condition or Neuro-Degenerative Diseases: An Immunocytochemical Approach

Kun¹,

Rosso²,

Canclini³

et al. 2012

Applications of Immunocytochemistry

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Section: Schwann Cells' Ribosomes Are Involved In Axonal Sprouting Ofmentioning

confidence: 90%

“…These mice carries a spontaneous mutation in Peripheral Myelin Protein-22 (PMP-22). All mice are recorded, numbered and genotyped following the method previously described (Rosso et al, 2010). Harems are formed after determining the stage of the female estral cycle.…”

Section: Animal Care and Maintenancementioning

confidence: 99%

The Schwann Cell-Axon Link in Normal Condition or Neuro-Degenerative Diseases: An Immunocytochemical Approach

Kun¹,

Rosso²,

Canclini³

et al. 2012

Applications of Immunocytochemistry

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“…All mice are recorded, numbered and genotyped following the method previously described [Rosso et al, 2010]. Our Trembler-J (B6.…”

Section: Animal Care and Maintenancementioning

confidence: 99%

“…These mice carry a spontaneous mutation in Peripheral Myelin Protein 22 (PMP-22). All mice are recorded, numbered and genotyped following the method previously described [Rosso et al, 2010]. All animals live under controlled temperature and 12-h light/dark cycle.…”

Section: Animal Care and Maintenancementioning

confidence: 99%

F‐actin distribution at nodes of Ranvier and Schmidt‐Lanterman incisures in mammalian sciatic nerves

et al. 2012

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Very little is known about the function of the F-actin cytoskeleton in the regeneration and pathology of peripheral nerve fibers. The actin cytoskeleton has been associated with maintenance of tissue structure, transmission of traction and contraction forces, and an involvement in cell motility. Therefore, the state of the actin cytoskeleton strongly influences the mechanical properties of cells and intracellular transport therein. In this work, we analyze the distribution of F-actin at Schmidt-Lanterman Incisures (SLI) and nodes of Ranvier (NR) domains in normal, regenerating and pathologic Trembler J (TrJ/+) sciatic nerve fibers, of rats and mice. F-actin was quantified and it was found increased in TrJ/+, both in SLI and NR. However, SLI and NR of regenerating rat sciatic nerve did not show significant differences in F-actin, as compared with normal nerves. Cytochalasin-D and Latrunculin-A were used to disrupt the F-actin network in normal and regenerating rat sciatic nerve fibers. Both drugs disrupt F-actin, but in different ways. Cytochalasin-D did not disrupt Schwann cell (SC) F-actin at the NR. Latrunculin-A did not disrupt F-actin at the boundary region between SC and axon at the NR domain. We surmise that the rearrangement of F-actin in neurological disorders, as presented here, is an important feature of TrJ/+ pathology as a Charcot-Marie-Tooth (CMT) model.

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“…Se empleó una barra de madera de 2.5 cm de diámetro a 70 cm del piso. Los animales se colocaron sobre la barra durante 1 minuto y fueron evaluados por dos personas entrenadas, con la siguiente escala (adaptado de Rosso et al, 2010); (1) movilidad normal y buen agarre; (2) reducida movilidad y buen agarre; (3) reducida movilidad y agarre medio; (4) ninguna movilidad y el agarre débil; (5) alteración de pie, sin agarre. Las sesiones se realizaron en la tarde, en una habitación iluminada con luz artificial blanca y aislada acústicamente.…”

Section: Prueba De La Barraunclassified

La memoria espacial, y los niveles de BDNF en el hipocampo, disminuyen en ratas adolescentes deprimidas farmacológicamente con reserpina

Ruiz

Calliari

Genovese

et al. 2021

PSICO

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Los cuadros depresivos en humanos cursan con significativas alteraciones en la memoria espacial. Este trabajo estudió esta función en un modelo animal de depresión. Ratas Wistar (n=28), machos y hembras, fueron administradas con reserpina (0.0 o 1.0 mg/kg/cada 2 días por cuatro días). Luego del tratamiento se evaluó la función motora a través de la prueba de la barra y la respuesta de ansiedad a través de un laberinto elevado en cruz. Posteriormente, los animales fueron entrenados durante una semana (en sesiones diarias de 5 minutos) en un laberinto de múltiples T que indaga la memoria espacial. Luego se repitió la evaluación en el laberinto elevado en cruz y los animales fueron sacrificados para disecar el hipocampo y medir los niveles de BDNF a través de western blot. En la prueba de la barra los animales tratados con reserpina tuvieron una respuesta motora con igual agarre, pero menor movilidad, que los controles. En ambas tomas del laberinto elevado en cruz los animales tratados con reserpina pasaron significativamente menos tiempo en los brazos abiertos y exhibieron una latencia más elevada para entrar por primera vez en los brazos cerrados. Los animales tratados con reserpina tardaron significativamente más tiempo en resolver el laberinto de múltiples T que los controles, y cometieron más errores, efecto que estuvo asociado a niveles menores de BDNF en el hipocampo. Estos resultados sugieren que la reserpina indujo una depresión experimental, sin compromiso motor, que estuvo asociada a déficits en la adqui-sición de información espacial.

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Early phenotypical diagnoses in Trembler-J mice model

Cited by 11 publications

References 30 publications

The Schwann Cell-Axon Link in Normal Condition or Neuro-Degenerative Diseases: An Immunocytochemical Approach

The Schwann Cell-Axon Link in Normal Condition or Neuro-Degenerative Diseases: An Immunocytochemical Approach

F‐actin distribution at nodes of Ranvier and Schmidt‐Lanterman incisures in mammalian sciatic nerves

La memoria espacial, y los niveles de BDNF en el hipocampo, disminuyen en ratas adolescentes deprimidas farmacológicamente con reserpina

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