Early pathogenesis of Autographa californica
            multiple nucleopolyhedrovirus and Helicoverpa zea
            single nucleopolyhedrovirus in Heliothis virescens: a comparison of the ‘M’ and ‘S’ strategies for establishing fatal infection

Washburn, Jan O.; Trudeau, D.; Wong, Jeffrey A.; Volkman, Loy E.

doi:10.1099/vir.0.18701-0

Cited by 54 publications

(38 citation statements)

References 29 publications

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“…Midgut cell sloughing may have been an important hostmediated selection pressure influencing NPV evolution, with virion production in the midgut epithelium being selected against due to its devastating effects on gregarious host populations. The success of lepidopteran baculoviruses might be due to their selection for ability to invade internal tissues so that a larger inoculum of virus could be produced (21,22,67).…”

Section: ϫ45mentioning

confidence: 99%

Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Lauzon

Lucarotti

Krell

et al. 2004

J Virol

132

124

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All fully sequenced baculovirus genomes, with the exception of the dipteran Culex nigripalpus nucleopolyhedrovirus (CuniNPV), have previously been from Lepidoptera. This study reports the sequencing and characterization of a hymenopteran baculovirus, Neodiprion lecontei nucleopolyhedrovirus (NeleNPV), from the redheaded pine sawfly. NeleNPV has the smallest genome so far published (81,755 bp) and has a GC content of only 33.3%. It contains 89 potential open reading frames, 43 with baculovirus homologues, 6 identified by conserved domains, and 1 with homology to a densovirus structural protein. Average amino acid identity of homologues ranged from 19.7% with CuniNPV to 24.9% with Spodoptera exigua nucleopolyhedrovirus. The conserved set of baculovirus genes has dropped to 29, since NeleNPV lacks an F protein homologue (ac23/ ld130). NeleNPV contains 12 conserved lepidopteran baculovirus genes, including that for DNA binding protein, late expression factor 11 (lef-11), polyhedrin, occlusion derived virus envelope protein-18 (odv-e18), p40, and p45, but lacks 21 others, including lef-3, me53, immediate early gene-1, lef-6, pp31, odv-e66, few polyhedra 25k, odv-e25, protein kinase-1, fibroblast growth factor, and ubiquitin. The lack of identified baculovirus homologues may be due to difficulties in identification, differences in host-virus interactions, or other genes performing similar functions. Gene parity plots showed limited colinearity of NeleNPV with other baculoviruses, and phylogenetic analysis indicates that NeleNPV may have existed before the lepidopteran nucleopolyhedrovirus and granulovirus divergence. The creation of two new Baculoviridae genera to fit hymenopteran and dipteran baculoviruses may be necessary.

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Section: ϫ45mentioning

confidence: 99%

Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Lauzon

Lucarotti

Krell

et al. 2004

J Virol

132

124

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“…Alphabaculoviruses have been divided into single nucleopolyhedrovirus (SNPV) and multiple nucleopolyhedrovirus (MNPV) occlusions. The M or S refers to whether the ODVs contain multiply or singly enveloped nucleocapsids, respectively (33). The MNPVs have been isolated only from Lepidoptera (23).…”

mentioning

confidence: 99%

“…It has been proposed that the M phenotype has an advantage over the S phenotype. Although the establishment of primary infections may be the same, the presence of multiple virions in MNPVs accelerated the onset of systemic infections (32,33).…”

mentioning

confidence: 99%

Autographa californica Multiple Nucleopolyhedrovirus Ac92 (ORF92, P33) Is Required for Budded Virus Production and Multiply Enveloped Occlusion-Derived Virus Formation

Passarelli

2010

J Virol

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The Autographa californica multiple nucleopolyhedrovirus orf92 (p33), ac92, is one of 31 genes carried in all sequenced baculovirus genomes, thus suggesting an essential function. Ac92 has homology to the family of flavin adenine dinucleotide-linked sulfhydryl oxidases and is related to the ERV/ALR family of sulfhydryl oxidases. The role of ac92 during virus replication is unknown. Ac92 was associated with the envelope of both budded and occlusion-derived virus (ODV). To investigate the role of Ac92 during virus replication, an ac92-knockout bacmid was generated through homologous recombination in Escherichia coli. Titration and plaque assays showed no virus spread in ac92-knockout bacmid DNA-transfected insect cells. Deletion of ac92 did not affect viral DNA replication. However, ac92-knockout bacmid DNAtransfected cells lacked multiply enveloped occlusion-derived nucleocapsids; instead, singly enveloped nucleocapsids were detected. To gain insight into the requirement for sulfhydryl oxidation during virus replication, a virus was constructed in which the Ac92 C 155 XXC 158 amino acids, important for sulfhydryl oxidase activity, were mutated to A 155 XXA 158 . The mutant virus exhibited a phenotype similar to that of the knockout virus, suggesting that the C-X-X-C motif was essential for sulfhydryl oxidase activity and responsible for the altered ODV phenotype.Baculoviruses infect insects and are characterized by a circular double-stranded DNA genome ranging from 80 to 180 kbp (10), which is packaged within a rod-shaped capsid and surrounded by an envelope. Two types of virions are produced during the baculovirus replication cycle: the budded virus (BV) and the occlusion-derived virus (ODV). Although the nucleocapsids are similar in the two, the envelopes differ in composition, reflecting their different functions during infection (4). The virions have distinct tissue tropism: ODVs infect midgut epithelial cells up to 10,000-fold more efficiently than do BVs, whereas BVs infect cultured cells 1,000-fold more efficiently than do ODVs (24).Alphabaculovirus and Betabaculovirus are two genera of Baculoviridae, based primarily on occlusion body morphology and phylogeny (11). Alphabaculoviruses have been divided into single nucleopolyhedrovirus (SNPV) and multiple nucleopolyhedrovirus (MNPV) occlusions. The M or S refers to whether the ODVs contain multiply or singly enveloped nucleocapsids, respectively (33). The MNPVs have been isolated only from Lepidoptera (23). It has been proposed that the M phenotype has an advantage over the S phenotype. Although the establishment of primary infections may be the same, the presence of multiple virions in MNPVs accelerated the onset of systemic infections (32, 33).The Autographa californica MNPV (AcMNPV) open reading frame 92 (orf92) (ac92, p33) is one of 31 core genes present in all sequenced baculovirus genomes (24). Previous studies showed that Ac92 and its homologs, Culex nigripalpus NPV orf14 and Helicoverpa armigera SNPV orf80, are associated with the ODV (3, 8, 18). Pre...

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“…Berdasarkan jumlah nukleokapsid, NPV dibedakan menjadi dua kelompok, yaitu single nukleokapsid (SNPV) dan multi nucleokapsid (MNPV) (Washburn et al, 2003;Inceoglu et al, 2006). Pada kelompok SNPV tiap envelop berisi satu nuckleokapsid, sedangkan pada MNPV berisi 2 -200 nukleokapsid (Kalmakoff dan Ward, 2003).…”

Section: Karakter Morfologi Npvunclassified

PROSPEK PENGEMBANGAN BIOINSEKTISIDA NUCLEOPOLYHEDROVIRUS (NPV) UNTUK PENGENDALIAN / Prospect of Development of Nucleopolyhedrovirus (NPV) Bioinsecticide Against

Samsudin¹

2017

PSP

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ABSTRAKPenggunaan insektisida kimia untuk mengendalikan hama tanaman perkebunan terbukti kurang efektif, tidak praktis, mahal, dan mencemari lingkungan. Virus patogen serangga (VPS) terutama nucleopolyhedrovirus (NPV) memiliki potensi untuk dikembangkan menjadi bioinsektisida yang efektif, efesien dan ramah lingkungan. NPV memiliki kemampuan bertahan di alam karena partikel virusnya terbungkus oleh polihedra dan mampu menyebar secara alami melalui proses penularan vertikal dan horisontal. Larva yang mati terinfeksi NPV sering ditemukan menggantung dengan kedua tungkai semu bagian abdomen menempel pada daun atau ranting tanaman. Penelitian untuk meningkatkan virulensi, spektrum inang dan persistensinya telah dilakukan untuk mengatasi beberapa kelemahan NPV apabila dikembangkan menjadi bioinsektisida. Beberapa NPV yang menginfeksi hama tanaman perkebunan dan berpotensi untuk dikembangkan di Indonesia, antara lain: Spodoptera litura NPV (SlNPV), S. exigua NPV (SeNPV), Helicoverpa armigera NPV (HaNPV), Sethotosea asigna NPV (SaNPV), Hyposidra talaca NPV (HtNPV) dan Maenas maculifascia NPV (MmNPV). Berdasarkan beberapa keunggulan NPV dibandingkan dengan insektisida kimia, maka pengembangan bioinsektisida NPV untuk pengendalian hama tanaman perkebunan di Indonesia memiliki prospek yang sangat baik.

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Early pathogenesis of Autographa californica multiple nucleopolyhedrovirus and Helicoverpa zea single nucleopolyhedrovirus in Heliothis virescens: a comparison of the ‘M’ and ‘S’ strategies for establishing fatal infection

Cited by 54 publications

References 29 publications

Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Autographa californica Multiple Nucleopolyhedrovirus Ac92 (ORF92, P33) Is Required for Budded Virus Production and Multiply Enveloped Occlusion-Derived Virus Formation

PROSPEK PENGEMBANGAN BIOINSEKTISIDA NUCLEOPOLYHEDROVIRUS (NPV) UNTUK PENGENDALIAN / Prospect of Development of Nucleopolyhedrovirus (NPV) Bioinsecticide Against

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