Early Ovule Development Following Self- and Cross-pollinations in Eucalyptus globulus Labill. ssp. globulus

Pound, Leanne M.; Wallwork, M. A. B.; Potts, Brad M.; Sedgley, Margaret

doi:10.1093/aob/mcf089

Cited by 31 publications

(30 citation statements)

References 18 publications

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“…One locule from all flowers harvested at 2, 4 and 6 weeks after pollination was fixed in 3% glutaraldehyde as described previously (Pound et al 2002b). Following fixation, some locules were processed further, including one locule from three self-and crosspollinated flowers from three trees harvested 2 weeks after pollination, and one locule from a single self-and cross-pollinated flower from the same trees harvested 4 weeks after pollination.…”

Section: Field Harvests and Microscopy Workmentioning

confidence: 99%

“…Following fixation, some locules were processed further, including one locule from three self-and crosspollinated flowers from three trees harvested 2 weeks after pollination, and one locule from a single self-and cross-pollinated flower from the same trees harvested 4 weeks after pollination. The ovules from these samples were dehydrated, embedded in glycol methacrylate, serially longitudinally sectioned and stained as described in Pound et al (2002b). The remaining intact fixed locules were dehydrated through methoxy-ethanol to ethanol and stored for observation.…”

Section: Field Harvests and Microscopy Workmentioning

confidence: 99%

“…E. spathulata and E. platypus have both pre-and post-zygotic barriers to selfpollinated seed production as pollen tube penetration of ovules and ovule fertilisation was reduced following selfcompared with cross-pollination, and few self-fertilised ovules developed past zygote division (Sedgley and Granger 1996). E. globulus has also shown both preand post-zygotic barriers to self-pollinated seed production, with slightly fewer ovules penetrated by pollen tubes following self-compared with cross-pollination, and the majority of ovule abortion occurring after fertilisation (Pound et al 2002a(Pound et al , 2002b. This evidence supports the notion that late pre-and post-zygotic sterility systems are not as rare as once thought (Seavey and Bawa 1986).…”

Section: Introductionmentioning

confidence: 99%

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Pollen tube growth and early ovule development following self- and cross-pollination in Eucalyptus nitens

Pound

Wallwork

Potts

et al. 2003

Sexual Plant Reproduction

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Controlled self-and cross-pollinations were conducted on flowers of five mature Eucalyptus nitens trees. Levels of self-sterility of the trees ranged from 25.8 to 93.6%. Pollen tube numbers in styles and ovule penetration by pollen tubes was investigated 2 weeks after pollination by fluorescence microscopy. There were no significant differences between treatments in the number of pollen tubes present in styles or in the percentage of ovules penetrated by pollen tubes. Embryology of material harvested 2 and 4 weeks after pollination was investigated by bright-field microscopy. Fertilisation had taken place by 2 weeks after pollination with nearly every ovule showing evidence of fertilisation. Cross-pollination resulted in a greater proportion of healthy, developing ovules, at both 2 and 4 weeks after pollination, compared with self-pollination. The proportion of degenerating ovules increased from 2 to 4 weeks after pollination. The reduced ability of E. nitens to set self-pollinated seed compared with cross-pollinated seed appears to be controlled by a post-zygotic mechanism. Differences in ovule size may potentially assist in the identification of trees incapable of setting self-pollinated seed.

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Section: Field Harvests and Microscopy Workmentioning

confidence: 99%

Section: Field Harvests and Microscopy Workmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pollen tube growth and early ovule development following self- and cross-pollination in Eucalyptus nitens

Pound

Wallwork

Potts

et al. 2003

Sexual Plant Reproduction

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“…There was no difference between self-and cross-pollination in pollen tube growth and fertilized ovules for Eucalyptus regnans . For E. globulus, there were more healthy, fertilized ovules from cross-compared to self-pollination, and the mechanism of self-incompatibility appeared to have both late pre-and postzygotic components (Pound et al 2002a). Another study to investigate the site of action of incompatibility found no difference in number of pollen tubes in the style (prezygotic) but, in contrast, almost complete lack of self-seed production (post-zygotic) compared with cross-pollinated seed (Pound et al 2002b).…”

Section: Introductionmentioning

confidence: 98%

Comparison of three pollination methods for Eucalyptus argophloia, a small-flowered eucalypt

Randall¹,

Walton²,

Lee³

et al. 2014

Annals of Forest Science

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Abstract& Context Most studies assess pollination success at capsule maturity, and studies of pre-zygotic processes are often lacking. & Aims This study investigates the suitability of controlled pollination for a potential forestry plantation species, Eucalyptus argophloia, by examining pre-and post-zygotic pollination success. & Methods Pollen tube development, capsule set and seed set are compared following three-stop pollination, artificially induced protogyny (AIP), AIP unpollinated and open pollination. The fecundity of stored pollen was compared with that of fresh pollen. & Results Three-stop pollination, AIP and open pollination had similar numbers of pollen tubes, but AIP unpollinated had none. Open pollination produced significantly more capsules and total number of seeds than the other treatments. There were significantly more seeds per retained capsule for the open pollination and three-stop pollination treatments than for the AIP and AIP unpollinated pollination treatments. There were no significant differences relative to the age of pollen. & Conclusions Pre-zygotic success in terms of pollen tubes was similar for open-pollinated, three stop and AIP, but was not reflected in post-zygotic success when the open pollination and three-stop method produced significantly more seeds per retained capsule than the AIP treatments and open pollination yielded more seeds. Capsule set and total seed set for open pollination, and fewer capsules in controlled pollinations, may reflect physical damage to buds because of the small E. argophloia flowers. Suitable alternative breeding strategies other than controlled pollinations are discussed for this species.

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“…The degree of self-incompatibility is illustrated by the index of self-incompatibility 24 . Normally, in self-incompatible and selfcompatible plants, the index of self-incompatibility is greater than 80% and less than 50%, respectively 37 . In self-incompatible plants, self-pollination lowered the seed-setting rate 16 whereas cross-pollinated plants gave a higher fruit setting rate 35,36,38,39 .…”

Section: Self-incompatibility Testmentioning

confidence: 99%

Pollen viability, germination, and seed setting of Nelumbo nucifera

Khatfan¹,

Zuo²,

Chen³

et al. 2014

ScienceAsia

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In tropical countries, low seed setting is a major problem when cultivating lotus (Nelumbo nucifera Gaertn.). This study determined seed setting in six wild lotus cultivars: Sai Rung (SR), Sam Roi Yot (SY), Klong Yong, Puttha Monthon (PM), Rachine (RC), and Nakhon Sawan (NW). In nature, the seed-setting rates range from 52-78%. From a stigma removal study, only a 53% seed-setting rate by self-pollination was recorded in the SR cultivar. This result was similar to the seed setting of self-pollinated PM, although high viability of PM pollen from day 2 full bloom flowers was observed. Self-incompatibility was studied in RC and NW cultivars. The indices of self-incompatibility of RC and NW were both 96% indicating that self-incompatibly affects low seed setting in these two cultivars. The results showed that the low seed-setting rate also came from seed abortion. A high value of self-incompatibility was observed in RC and NW cultivars, but not in the other cultivars studied.

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Early Ovule Development Following Self- and Cross-pollinations in Eucalyptus globulus Labill. ssp. globulus

Cited by 31 publications

References 18 publications

Pollen tube growth and early ovule development following self- and cross-pollination in Eucalyptus nitens

Pollen tube growth and early ovule development following self- and cross-pollination in Eucalyptus nitens

Comparison of three pollination methods for Eucalyptus argophloia, a small-flowered eucalypt

Pollen viability, germination, and seed setting of Nelumbo nucifera

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