Early osteogenic differential protein profile detected by proteomic analysis in human periodontal ligament cells

Abstract

Our results suggest that the proteins identified in this study may be associated with the unique function of periodontal ligament cells in maintaining periodontal tissue homeostasis, thus providing a comprehensive reference for understanding and investigating in greater detail the molecular mechanisms of periodontal ligament cells involved in periodontal regeneration.

Comparison of Human Dental Follicle Cells and Human Periodontal Ligament Cells for Dentin Tissue Regeneration

“…TBCA is a tubulin folding cofactor, which is essential for cell cytoskeleton function [38]. The up-regulated TBCA in PDLCs may be associated with the unique function of PDLCs in the maintenance of the periodontium as well [39].…”

Proteomic Characterization of Mesenchymal Stem Cell-Like Populations Derived from Various Tissue Types

“…During early cementoblastic/osteogenic differentiation of periodontal ligament stem cells, a total of 29 differentially expressed proteins were identifi ed by DIGE (Wu et al 2009 ) . Reduced expression of cytoskeletal proteins vimentin, caldesmon and tropomyosin is thought to relate to cytoskeletal rearrangements during differentiation processes while the regulation of binding partners caldesmon and tropomyosin also plays a role in cytokenesis as discussed earlier in the chapter.…”

Stem Cell Regulatory Gene Expression in Human Adult Dental Pulp and Periodontal Ligament Cells Undergoing Odontogenic/Osteogenic Differentiation

“…In our previous studies (3,5,6), we have shown that DPCs and PDLCs are similar with respect to morphology and expression of cell surface markers (Stro-1, CD146, CD29-Fluorescein isothiocyanate (FITC), CD34-FITC, CD44-FITC, and CD106-FITC) and that both possess odontogenic/osteogenic differentiation potential. Recently, we investigated the differential protein expression profile of human DPCs and PDLCs undergoing odontogenic/osteogenic differentiation by using Two dimensional difference gel electrophoresis and mass spectroscopy based proteomic approaches (5,6).…”

“…Recently, we investigated the differential protein expression profile of human DPCs and PDLCs undergoing odontogenic/osteogenic differentiation by using Two dimensional difference gel electrophoresis and mass spectroscopy based proteomic approaches (5,6). In odontoblast-like differentiation of DPCs, proteins involved in cell cycle regulation, protein synthesis and degradation, Ca 2+ homeostasis, signal transduction, translation, and cellular energy regulation have been shown to be differentially expressed (5).…”

“…In odontoblast-like differentiation of DPCs, proteins involved in cell cycle regulation, protein synthesis and degradation, Ca 2+ homeostasis, signal transduction, translation, and cellular energy regulation have been shown to be differentially expressed (5). In the osteogenic differentiation of PDLCs, nuclear proteins, cell membrane-bound molecules, cytoskeleton proteins, and cytoskeleton-associated proteins have been identified to be the main regulatory proteins involved in the early process of PDLCs differentiation (6). Differences in the protein expression profile between DPCs and PDLCs seen during the process of mineralization is closely related to the functional role of these dental derived cells in maintaining tissue homeostasis.…”