Early monitoring of human renal transplantations by isoenzyme activities in urines

Bourbouze, Richard; Gluckman, Jean-Claude; Frantz, P; Paraire, Marie; Baumann, Fritz; Luciani, J; Percheron, F; Legrain, M

doi:10.1016/0009-8981(85)90332-8

Cited by 17 publications

(9 citation statements)

References 20 publications

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“…The difference between AGR and ATN group was not statistically significant. These results are also supported by earlier reports, which suggest that excretion of NAG and its isoenzyme fails to identify a specific cause of increased enzymuria in patients after renal transplantation [13][14][15]. Independently of the elevated NAG excretion, increased excretion of brush border enzymes-AAP and GGT were observed in AGR and ATN groups in the early post-transplant period.…”

Section: Discussionsupporting

confidence: 92%

“…NAG-B isoenzyme has been used in determining the source of increased enzymuria. Contrary to NAG, released also from degranulated neutrophils [1,2], NAG-B specifically originates from the kidney [13]. In our study the NAG and NAG-B excretion was significantly higher in AGR and ATN groups than in SGF patients; the highest NAG and NAG-B excretion was observed in the AGR group.…”

Section: Discussionmentioning

confidence: 49%

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Enzymuria and low molecular weight protein excretion as the differentiating marker of complications in the early post kidney transplantation period

et al. 2006

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Renal function in the early post-transplantation period depends largely on factors affecting the kidney prior to implantation. Function of the graft may be also disturbed by the most common complications of the early post-operative period such as acute graft rejection (AGR), acute tubular necrosis (ATN) and may be modified by nephrotoxic action of cyclosporine A (CsA). Evaluation of excretion of enzymes and low molecular weight proteins (LMWP) may help in the differentiation of these complications. Aim Comparison of the urinary excretion of markers of tubular injury in patients with AGR, ATN, or patients with stable graft function (SGF) was made and differences between groups and correlations between markers and cold ischemia time (CIT), warm ischemia time (WIT) and blood trough level of cyclosporine A (CsA0) were determined. Material and methods In 60 cadaveric renal allograft recipients in the early post-transplantation period urinary excretion of N-acetyl-beta-D-glucosaminidase (NAG) and B isoenzyme (NAG-B), alanylaminopeptidase (AAP), gamma-glutamyltransferase (GGT), alpha and pi isoenzymes of glutathione S-transferase (alpha-GST, pi-GST), retinol binding protein (RBP) and beta2- microglobulin (beta2M), were analyzed. Results NAG and NAB-B activities were higher in ATN (P<0.05, P<0.01) and in AGR (P<0.005, P<0.02) than in SGF. Excretion of pi-GST was higher in AGR than in SGF (P<0.0002) or ATN (P<0.007). CIT and WIT in patients with ATN were higher (P<0.05) than in SGF group. In ATN patients, correlations of CIT with RBP (P<0.05) and pi-GST (P<0.05), and WIT with RBP (P<0.05), and pi-GST (P<0.001) were found. Conclusions High urinary NAG and NAG B excretion characterizes ATN and AGR patients. Evaluating urinary excretion of pi-GST may be helpful in differentiating AGR from ATN. However, taking into account ischemia time is necessary in interpreting the pi-GST value in early post transplant period.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 49%

Enzymuria and low molecular weight protein excretion as the differentiating marker of complications in the early post kidney transplantation period

et al. 2006

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“…of cells x 153/125 x 104) to give the total cells/ml. 4 Cell samples were cytocentrifuged at 900 rpm for 2 minutes, fixed, stained with Dif-Quick Stain (Baxter, McGraw Park, IL), coverslipped using permount, and examined microscopically under oil at 1000x magnification. Differential cell counts were obtained by counting 100 cells and marking the cells as macrophages, neutrophils, lymphocytes, eosinophils, or other cells [15].…”

Section: Methodsmentioning

confidence: 99%

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

Mack

Griffith

Riling

et al. 1995

Lung

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N-acetyl-beta(beta)-D-glucosaminidase is a lysosomal enzyme secreted by alveolar macrophages in response to phagocytosis of particulate material. Alveolar macrophages participate in the degradation and fibrosis of pulmonary tissue that results in pneumoconiosis. Known quantities of four characterized respirable dusts were bronchoscopically placed into the right caudal lung lobe of macaque monkeys. Bronchoalveolar lavage (BAL) samples were collected from dust-exposed right lung and unexposed left lung of the same individuals at 2-week intervals for 12 weeks after dust instillation. The samples were tested for N-acetyl-beta-D-glucosaminidase activity to determine if the enzyme levels could serve as an indicator of pulmonary injury induced by generic coal dusts when compared to known fibrogenic and nuisance dusts. Installation of generic quartz, anthracite, or TiO2 dusts produced significant elevations of enzyme activity and increased numbers of macrophages in the dust-exposed lobes. Elevations in enzymatic activity and macrophage numbers were greatest in response to generic quartz dust. These results suggest that quantitative levels of N-acetyl-beta-D-glucosaminidase activity may be a useful indicator of acute and chronic lung injury following exposure to fibrogenic and nonfibrogenic dusts.

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“…Bourbouze et al [ 13] stud ied 41 episodes of acute rejection after renal transplantation and confirmed that urinary NAG is a useful indicator of rejection but since there was a positive correlation be tween total NAG and NAG B the measure ments of isoenzymes did not seem to provide additional information in the early monitor ing of renal transplantation patients. Yuen et al [14] found in their study on 20 renal transplant patients an increased urinary NAG activity in stable renal transplant pa tients with a normal isoenzyme profile.…”

Section: Introductionmentioning

confidence: 99%

“…During the last 5 years there have been a few reports [12][13][14][15][16] describing possible clini cal utility of NAG isoenzyme analysis. The separation of NAG A and NAG B has been performed by ion-exchange chromatography or isoelectric focusing, which both are time consuming and not easily adapted to use in clinical routine.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Immunoassay of ß-Hexosaminidase Isoenzymes in Human Urine and Renal Cortex with Monoclonal Antibodies

Hultberg

Isaksson²

1989

Enzyme

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Enzyme immunoassay (EIA) methods with monoclonal antibodies specific for N-acetyl-ß-D-glucosaminidase (NAG) isoenzymes A and B in human urine are presented. The proportion of NAG B obtained with the EIA methods was similar to that found with ion-exchange chromatography. In fresh human control urines, NAG B was found to be approximately 20% of the total NAG activity. A significant correlation was obtained between total NAG activity in human urine assayed with a conventional enzyme substrate method and the total NAG activity obtained as the sum of NAG A and NAG B analyzed with the EIA methods. Total NAG activity with the latter (EIA) methods showed about 30% higher values than found by the enzyme substrate method, which probably was due to inhibitors of NAG activity present in urine did not interfere with the EIA methods. The content of NAG A and NAG B in renal cortex was determined with the EIA methods. NAG B accounted for about 20% of the total NAG activity, which was similar to that found in fresh human urines.

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Early monitoring of human renal transplantations by isoenzyme activities in urines

Cited by 17 publications

References 20 publications

Enzymuria and low molecular weight protein excretion as the differentiating marker of complications in the early post kidney transplantation period

Enzymuria and low molecular weight protein excretion as the differentiating marker of complications in the early post kidney transplantation period

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

Enzyme Immunoassay of ß-Hexosaminidase Isoenzymes in Human Urine and Renal Cortex with Monoclonal Antibodies

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