Early lipid changes in acute kidney injury using SWATH lipidomics coupled with MALDI tissue imaging

Rao, Sangeetha; Walters, Kelly B.; Wilson, Landon; Chen, Bin; Bolisetty, Subhashini; Graves, David E.; Barnes, Stephen; Agarwal, Anupam; Kabarowski, Janusz H.

doi:10.1152/ajprenal.00100.2016

Cited by 52 publications

(57 citation statements)

References 46 publications

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“…been restored [28]. Other results stated that MSCs administration to rats with acute kidney injury significantly restored renal functional parameters and hastened functional recovery [27,29,30].…”

Section: Comparison Among Different Groupsmentioning

confidence: 97%

Bone Marrow Derived Mesenchymal Stem Cell Therapy in Induced Acute Renal Injury in Adult Male Albino Rats

El-Wahab¹,

A²,

A³

et al. 2017

J Cytol Histol

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Background and objectives: Acute renal injury (ARI) is a serious clinical problem without established regimen for treatment. Mesenchymal stem cells (MSCs) are undifferentiated cells that can differentiate and give rise to other cell types. The aim of the present work is to study the possible therapeutic role of MSCs in the treatment of the deleterious changes occurred in renal cortex and medulla of adult male albino rat model of ischemia/reperfusion (I/R) ARI.Material and methods: Forty adult male albino rats were used in this study. Animals were divided into four groups, 10 animals for each group. Group I is control sham-operated group. Three experimental groups were all subjected to I/R injury by clamping both renal pedicles for 40 min. These animals were further divided into three subgroups. Group II is non-MSC treated group. Group III (MSC treated group (local injection)): I/R animals received bromodeoxyuridine labeled BM-MSCs locally in renal cortex immediately after removal of the clamps and confirmation of reflow. Group IV (MSC treated group (systemic injection)): I/R animals that received single intravenous injection of bromdeoxyouridine labeled BM-MSCs in tail vein immediately after removal of the clamps and confirmation of reflow. Animals were sacrificed after 3 days of intervention. Serological measurements included serum urea and creatinine. Kidney specimens were processed for H&E, PCNA and Caspase-3. Real time RT-PCR study was done for TNF α and IL-6 gene expression.Results: Cortical and medullary renal tissue exhibited marked improvement histologically and serologically after MSCs treatment. The improvement was more obvious in case of systemic treatment. Conclusion:The present study shows the ability of MSCs to repair the structural and functional renal damage after I/R, Systemic route is more beneficial than local route. This may pave the future for therapeutic use of MSCs in the treatment of acute renal injury.

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Section: Comparison Among Different Groupsmentioning

confidence: 97%

Bone Marrow Derived Mesenchymal Stem Cell Therapy in Induced Acute Renal Injury in Adult Male Albino Rats

El-Wahab¹,

A²,

A³

et al. 2017

J Cytol Histol

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“…In addition, SWATH‐MS has also been successfully applied to lipidomics, for both lipid characterization and quantification , and similarly to the field of proteomics, comprehensive lipid libraries are being generated that can be applied for further studies . More recently, SWATH acquisition was combined with an autosampler‐in‐needle‐derivatization with p ‐toluenesulfonylhydrazine (TSH) to identify and quantify aldehydes and ketones, which could be also an interesting strategy for the study of protein carbonylation .…”

Section: Swath‐ms: a Translational Toolmentioning

confidence: 99%

SWATH‐MS as a tool for biomarker discovery: From basic research to clinical applications

2017

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In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in particular associated with the use of data-independent acquisition, it is time to perform an overview of this growing field with special focus on one of the most promising approaches: SWATH-MS, and to present future perspectives for its application as a translational tool. Therefore, a summary of this technique is presented focusing on two key relevant concepts associated with its application in biomarker discovery: the protein library and the individual digital maps concepts. It is also the purpose of this review to document the likely impact of SWATH-MS in both fundamental and translational research including biomarker identification and creation of diagnostic tools. To that end, the two concepts referred above were integrated with ongoing technical developments. Finally, some of the current restrictions for the implementation of SWATH-MS on a large scale are identified, and potential solutions presented, namely protocol standardization combined with the use of the proper standards.

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“…This information helps to establish mechanism and suggests possible physiological consequences of those lipid changes. An example from our group 60 is shown in Figure 12. Several ether-linked phospholipids were found to be increased early after ischemia-reperfusion kidney injury in mice using MS/ MS ALL lipidomics.…”

Section: Imaging-msmentioning

confidence: 99%

Training in metabolomics research. II. Processing and statistical analysis of metabolomics data, metabolite identification, pathway analysis, applications of metabolomics and its future

Barnes¹,

Benton²,

Casazza³

et al. 2016

J. Mass Spectrom.

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Journal of MASS SPECTROMETRYMetabolomics is perhaps the most challenging of the -omics fields, given the complexity of an organism's metabolome and the rapid rate at which it changes. When one sets out to study metabolism there are numerous dynamic variables that can influence metabolism that must be considered. Recognizing the experimental challenges confronting researchers who undertake metabolism studies, workshops like the one at University of Alabama at Birmingham have been established to offer instructional guidance. A summary of the UAB course training materials is being published as a two-part Special Feature Tutorial. In this month's Part I the authors discuss details of good experimental design and sample collection and handling. In an upcoming Part II, the authors discuss in detail the various aspects of data analysis. in 2006, where her research focused on dietary and physical activity education interventions in adolescents. As a dietitian, a researcher and scientist her research team has conducted various clinical studies in the pediatric population investigating the contribution of dietinduced metabolic alterations on the interface between bone, muscle and fat. Employing strength training intervention during the critical developmental stage surrounding puberty to optimize the protective effect of the musculoskeletal system on feed forward effects across systems shifts the focus from the dogmatic concept of adiposity reduction. Most recently, she has begun to investigate the integral interactions encompassing the metabolome. She organized the young investigators' section of the workshop. Sara J. Cooper, Ph.D. Dr. Cooper is a Faculty Investigator at the HudsonAlpha Institute for Biotechnology. Her area of expertise lies at the intersection of metabolomics and genomics -her graduate work was focused on genomics, genetics, gene regulation, and development of high-throughput assays and data analysis pipelines for the large datasets. As a postdoctoral fellow with Stan Fields, she applied this experience to the new and growing field of metabolomics. Her current research projects include metabolomics-based methods for biomarker identification in pancreatic cancer, characterization of heritability of metabolite levels in yeast and human populations, and the use of yeast and human cell culture models to understand metabolic changes in human disease. She provided training in the use of GC-MS and 2D-GC in the workshop. identifying novel lipid-based mechanisms of immune regulation in autoimmunity and chronic inflammatory disease. He has established a method for lipid imaging in cryo-sections by MALDI-Imaging Mass Spectrometry using vacuum sublimation for matrix application. This technology is being applied to a number of areas, including identifying lipids modulating inflammatory processes in response to acute kidney injury, autoimmunity and kidney disease in Lupus, as well as those involved in the deterioration of ocular function with aging.Shuzhao Li, Ph.D. Dr. Li is an assistant professor at Emory University ...

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Early lipid changes in acute kidney injury using SWATH lipidomics coupled with MALDI tissue imaging

Cited by 52 publications

References 46 publications

Bone Marrow Derived Mesenchymal Stem Cell Therapy in Induced Acute Renal Injury in Adult Male Albino Rats

Bone Marrow Derived Mesenchymal Stem Cell Therapy in Induced Acute Renal Injury in Adult Male Albino Rats

SWATH‐MS as a tool for biomarker discovery: From basic research to clinical applications

Training in metabolomics research. II. Processing and statistical analysis of metabolomics data, metabolite identification, pathway analysis, applications of metabolomics and its future

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